PNO1 (NM_020143) Human Tagged ORF Clone

CAT#: RC203434

PNO1 (Myc-DDK-tagged)-Human partner of NOB1 homolog (S. cerevisiae) (PNO1)

Product Images

Western blot validation of overexpression lysate (Cat# LY412632) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203434 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PNO1 protein (Cat# TP303434). The protein was produced from HEK293T cells transfected with PNO1 cDNA clone (Cat# RC203434) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PNO1
• Synonyms: KHRBP1; RRP20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203434 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCCGAAATGGAAACGCAGAGCGCCAGGGCAGAGGAGGGCTTTACCCAGGTCACCCGCAAGGGTG
  GCCGACGGGCGAAGAAACGACAGGCTGAACAGCTGTCCGCAGCAGGAGAGGGCGGGGATGCGGGCCGCAT
  GGACACAGAGGAGGCCAGGCCGGCGAAGAGGCCCGTCTTCCCACCCCTCTGTGGGGACGGGCTCCTGAGT
  GGGAAAGAAGAAACAAGGAAAATTCCAGTCCCAGCTAACAGATACACACCATTGAAAGAAAACTGGATGA
  AGATATTTACTCCTATTGTGGAACATTTGGGACTTCAGATACGCTTTAACTTGAAATCAAGGAATGTAGA
  AATCAGGACTTGTAAAGAAACCAAGGATGTTAGTGCTCTGACAAAAGCAGCTGATTTTGTGAAAGCTTTT
  ATTCTCGGCTTTCAGGTGGAGGATGCACTTGCCCTCATCAGGTTGGATGACCTCTTCCTAGAGTCTTTTG
  AAATTACAGATGTTAAACCCCTAAAGGGAGACCATCTATCCAGGGCAATAGGAAGAATCGCTGGCAAAGG
  AGGAAAAACCAAATTCACCATAGAGAATGTGACACGGACAAGGATAGTTTTGGCTGATGTGAAAGTTCAC
  ATCCTTGGCTCCTTCCAAAATATCAAGATGGCAAGAACTGCCATTTGCAACCTAATCTTGGGAAATCCTC
  CTTCCAAGGTTTATGGCAATATTCGAGCTGTGGCTAGCAGATCAGCAGATCGATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203434 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESEMETQSARAEEGFTQVTRKGGRRAKKRQAEQLSAAGEGGDAGRMDTEEARPAKRPVFPPLCGDGLLS
  GKEETRKIPVPANRYTPLKENWMKIFTPIVEHLGLQIRFNLKSRNVEIRTCKETKDVSALTKAADFVKAF
  ILGFQVEDALALIRLDDLFLESFEITDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTRTRIVLADVKVH
  ILGSFQNIKMARTAICNLILGNPPSKVYGNIRAVASRSADRF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020143
• ORF Size: 756 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020143.4
• RefSeq Size: 1994 bp
• RefSeq ORF: 759 bp
• Locus ID: 56902
• UniProt ID: Q9NRX1
• Cytogenetics: 2p14
• Domains: KH
• MW: 27.9 kDa
• Gene Summary: Positively regulates dimethylation of two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA (PubMed:25851604).[UniProtKB/Swiss-Prot Function]