PSMG2 (NM_020232) Human Tagged ORF Clone

CAT#: RC203422

PSMG2 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) assembly chaperone 2 (PSMG2), transcript variant 1

Product Images

Circular map for RC203422

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PSMG2 (Cat# RC203422, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMG2 (Cat# TA808168)(1:2000). Positive lysates LY412568 (100ug) and LC412568 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY412568) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203422 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PSMG2
• Synonyms: CLAST3; HCCA3; HsT1707; MDS003; PAC2; TNFSF5IP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203422 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGTTCCCTGCGGGGAGTCGGCCCCCGACCTTGCCGGCTTCACCCTCCTAATGCCAGCAGTATCTG
  TTGGAAATGTTGGCCAGCTTGCAATGGATCTGATTATTTCTACACTGAATATGTCTAAGATTGGTTACTT
  CTATACCGATTGTCTTGTGCCAATGGTTGGAAACAATCCATATGCGACCACAGAAGGAAATTCAACAGAA
  CTTAGCATAAATGCTGAAGTGTATTCATTGCCTTCAAGAAAGCTGGTGGCTCTACAGTTAAGATCCATTT
  TTATTAAGTATAAATCAAAGCCGTTCTGTGAAAAACTGCTTTCCTGGGTGAAAAGCAGTGGCTGTGCCAG
  AGTCATTGTTCTTTCAAGCAGTCATTCATATCAGCGTAATGATCTGCAGCTTCGTAGTACTCCCTTCCGG
  TACCTACTTACACCTTCCATGCAAAAAAGTGTTCAAAATAAAATAAAGAGCCTTAACTGGGAAGAAATGG
  AAAAAAGCCGGTGCATTCCTGAAATAGATGATTCCGAGTTTTGTATCCGCATTCCGGGAGGAGGTATCAC
  AAAAACACTCTATGATGAAAGCTGTTCTAAAGAAATCCAAATGGCAGTTCTGCTGAAATTTGTTTCAGAA
  GGGGACAACATCCCAGATGCATTAGGTCTTGTTGAGTATCTTAATGAGTGGCTTCAGATACTCAAACCAC
  TTAGCGATGACCCCACAGTATCTGCCTCACGGTGGAAAATACCAAGTTCTTGGAGATTACTCTTTGGCAG
  TGGTCTTCCCCCTGCACTTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203422 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFVPCGESAPDLAGFTLLMPAVSVGNVGQLAMDLIISTLNMSKIGYFYTDCLVPMVGNNPYATTEGNSTE
  LSINAEVYSLPSRKLVALQLRSIFIKYKSKPFCEKLLSWVKSSGCARVIVLSSSHSYQRNDLQLRSTPFR
  YLLTPSMQKSVQNKIKSLNWEEMEKSRCIPEIDDSEFCIRIPGGGITKTLYDESCSKEIQMAVLLKFVSE
  GDNIPDALGLVEYLNEWLQILKPLSDDPTVSASRWKIPSSWRLLFGSGLPPALF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020232
• ORF Size: 792 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020232.3, NP_064617.2
• RefSeq Size: 1141 bp
• RefSeq ORF: 795 bp
• Locus ID: 56984
• UniProt ID: Q969U7
• Cytogenetics: 18p11.21
• MW: 29.4 kDa
• Gene Summary: Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.[UniProtKB/Swiss-Prot Function]