ZNF 556 (ZNF556) (NM_024967) Human Tagged ORF Clone

CAT#: RC203404

ZNF556 (Myc-DDK-tagged)-Human zinc finger protein 556 (ZNF556)

Product Images

Circular map for RC203404

• 

  Western blot validation of overexpression lysate (Cat# LY410961) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203404 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF 556
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203404 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACAGTGGTCTTTGAAGACGTGGTTGTGGATTTCACGCTGGAGGAGTGGGCCTTGCTGAATCCTG
  CTCAGAGAAAACTCTACAGAGATGTCATGCTGGAGACCTTCAAGCACCTGGCCTCAGTAGATAATGAGGC
  TCAGCTTAAAGCCAGTGGGTCTATTTCTCAGCAGGATACTTCTGGAGAAAAATTATCCCTCAAACAGAAA
  ATAGAAAAGTTCACAAGAAAGAATATATGGGCCTCCCTTTTAGGAAAAAATTGGGAAGAACATAGCGTTA
  AAGACAAGCACAACACCAAGGAGAGACATTTGAGAAATCCAAGGGTGGAGAGACCATGTAAAAGCAGTAA
  AGGTAATAAACGTGGAAGAACCTTCAGAAAGACTCGAAATTGTAATCGTCATCTGCGCAAGAATTGTTGT
  ACTAGTGTAAGACGGTACGAATGCAGTCAGTGTGGAAAACTCTTCACCCATTCCTCATCCCTGATAAGGC
  ACAAAAGAGCTCACTCTGGACAAAAATTATATAAATGTAAGGAATGTGGGAAAGCCTTCAGTCGCCCTTC
  CTACCTACAGACGCATGAGAAAACTCACAGTGGAGAGAAACCCTATGCCTGTCAATCTTGCGGGAAGACA
  TTTCTTCGTTCCCACTCTCTCACTGAACATGTAAGGACTCACACTGGAGAGAAACCCTACGAATGTGGGC
  AGTGTGGGAAAGGCTTCAGTTGTCCCAAATCCTTTCGCGCACATGTGATGATGCACGCCGGAGGGAGACC
  GTATGAGTGCAAGCACTGTGGGAAAGCCTTCAGGTGTCAGAAATCCTTTCGAGTCCATATGATCATGCAC
  GCCGGAGGGAGACCGTATGAGTGCAAGCAGTGTGGGAAAGCCTACTGCTGGGCAACATCCTTTCAACGAC
  ACGTGAGAATTCACAACGGGGAGAAACCCTATAAGTGTGGAAAATGCGGGAAAGCATTCGGTTGGCCCTC
  ATCCTTACACAAACACGCGAGAACGCATGCTAAAAAGAAACCTGTGAGTGGGGGCAGCGTGGGAAAGTCT
  TCCGCGAGGCCTCGCCCCTCCACAGATGTCAAATCACAAACTAGAGAGAAAGTCTATAAATGTGAAACGT
  GTGGGAAAACGTATGGTTGGTCCTCATCTTTACACAAACATGAGAGAAAGCACACTGGGGAGAAACCTGT
  AAATGCAGCCAGTGTGGGAAAACCTTCAGGCGGGCTTTGCTCTTCCAAAAATGTAAGAACGCAGATTGGA
  CAGAAGCCCAGTAAATGCGAAAAATGTGGGAAAGCTTTCAGTTGTCCCAAAGCCTTTCAAGGTCATGTGA
  GAAGTCACACAGGAAAGAAATCCTGTACATCTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203404 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDTVVFEDVVVDFTLEEWALLNPAQRKLYRDVMLETFKHLASVDNEAQLKASGSISQQDTSGEKLSLKQK
  IEKFTRKNIWASLLGKNWEEHSVKDKHNTKERHLRNPRVERPCKSSKGNKRGRTFRKTRNCNRHLRKNCC
  TSVRRYECSQCGKLFTHSSSLIRHKRAHSGQKLYKCKECGKAFSRPSYLQTHEKTHSGEKPYACQSCGKT
  FLRSHSLTEHVRTHTGEKPYECGQCGKGFSCPKSFRAHVMMHAGGRPYECKHCGKAFRCQKSFRVHMIMH
  AGGRPYECKQCGKAYCWATSFQRHVRIHNGEKPYKCGKCGKAFGWPSSLHKHARTHAKKKPVSGGSVGKS
  SARPRPSTDVKSQTREKVYKCETCGKTYGWSSSLHKHERKHTGEKPVNAASVGKPSGGLCSSKNVRTQIG
  QKPSKCEKCGKAFSCPKAFQGHVRSHTGKKSCTSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024967
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024967.3
• RefSeq Size: 1650 bp
• RefSeq ORF: 1371 bp
• Locus ID: 80032
• UniProt ID: Q9HAH1
• Cytogenetics: 19p13.3
• Protein Families: Transcription Factors
• MW: 51.5 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]