ASCC2 (NM_032204) Human Tagged ORF Clone

CAT#: RC203391

ASCC2 (Myc-DDK-tagged)-Human activating signal cointegrator 1 complex subunit 2 (ASCC2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ASCC2 (Cat# RC203391, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASCC2(Cat# TA502806). Positive lysates LY403150 (100ug) and LC403150 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY403150) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203391 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ASCC2 protein (Cat# TP303391). The protein was produced from HEK293T cells transfected with ASCC2 cDNA clone (Cat# RC203391) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASCC2
• Synonyms: ASC1p100; p100
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203391 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCTCTGCCCCTGGACCAACTCCAGATCACCCACAAGGACCCGAAGACAGGAAAGCTGAGGACTT
  CACCAGCGCTGCACCCCGAGCAGAAGGCAGACCGGTATTTTGTGTTATACAAACCGCCCCCTAAAGACAA
  CATTCCCGCCCTAGTGGAGGAGTACCTGGAACGCGCCACCTTCGTAGCCAATGACCTCGACTGGCTCCTG
  GCCTTGCCTCACGATAAATTCTGGTGCCAGGTGATCTTTGACGAGACTCTACAGAAGTGCCTGGACTCCT
  ACCTGCGCTATGTCCCCCGCAAATTCGACGAGGGGGTGGCCTCAGCCCCTGAGGTTGTTGACATGCAGAA
  GCGCCTCCATCGAAGTGTTTTTCTCACCTTCCTCCGCATGTCCACTCACAAGGAATCCAAAGATCACTTC
  ATTTCCCCTTCTGCGTTTGGAGAAATCCTCTACAATAACTTCCTCTTTGACATTCCAAAGATCCTGGACC
  TCTGCGTGCTCTTTGGAAAAGGCAACTCACCACTGCTCCAGAAGATGATAGGAAACATCTTTACACAGCA
  GCCAAGTTACTACAGTGACCTGGATGAAACCCTGCCTACCATCCTTCAGGTCTTCAGCAATATCCTCCAG
  CACTGTGGTTTGCAAGGGGACGGGGCCAATACCACACCCCAGAAGCTTGAGGAGAGGGGCCGATTGACCC
  CCAGTGACATGCCTCTCCTGGAATTAAAGGACATTGTTCTCTACCTTTGTGATACCTGCACCACACTTTG
  GGCCTTTCTGGATATCTTCCCTTTGGCTTGCCAGACCTTCCAGAAGCACGACTTTTGTTACAGACTAGCT
  TCCTTCTACGAAGCAGCAATTCCCGAAATGGAGTCTGCAATTAAGAAGAGGAGGCTTGAAGATAGCAAGC
  TTCTTGGTGACCTGTGGCAGAGGCTCTCCCATTCCAGGAAGAAGCTAATGGAGATTTTCCACATCATCCT
  GAACCAGATCTGCCTCCTTCCCATCCTAGAAAGCAGCTGTGACAACATTCAGGGCTTCATCGAAGAGTTC
  CTTCAGATCTTCAGCTCCTTGCTGCAGGAGAAGAGGTTCCTCCGGGACTATGATGCACTCTTCCCCGTGG
  CCGAAGACATCAGCTTGCTGCAGCAGGCCTCATCAGTCTTGGACGAGACGCGGACTGCCTACATCCTCCA
  GGCAGTCGAGAGTGCATGGGAAGGGGTGGACAGACGGAAAGCCACAGATGCTAAAGACCCATCGGTGATT
  GAGGAGCCTAATGGGGAGCCTAACGGGGTCACGGTGACAGCAGAGGCAGTCAGTCAAGCATCATCACATC
  CGGAGAACTCGGAGGAAGAGGAGTGCATGGGAGCAGCCGCGGCTGTGGGCCCTGCCATGTGTGGGGTGGA
  ACTGGACTCTCTCATCTCCCAAGTGAAGGACCTGCTGCCAGACCTTGGTGAGGGCTTCATCCTGGCCTGC
  CTGGAGTACTACCACTACGACCCAGAGCAGGTGATCAACAATATCCTGGAGGAGCGGCTGGCCCCCACCC
  TCAGCCAGCTGGACCGCAACCTAGACAGAGAAATGAAACCAGACCCTACACCCCTGCTGACGTCTCGCCA
  CAACGTCTTCCAGAATGACGAGTTTGATGTGTTCAGCAGGGACTCAGTAGACCTGAGCCGGGTGCACAAG
  GGCAAGAGCACCAGGAAGGAGGAAAACACGCGGAGTTTGCTGAACGACAAGCGTGCAGTGGCGGCACAGC
  GGCAGCGCTACGAGCAGTACAGCGTGGTGGTGGAGGAGGTGCCACTGCAGCCAGGCGAGAGCCTGCCCTA
  CCACAGTGTCTACTACGAGGATGAGTACGATGACACATACGATGGCAACCAGGTGGGCGCCAATGATGCA
  GACTCTGATGACGAGCTCATCAGCCGCAGGCCATTCACCATCCCTCAGGTGCTGAGAACCAAAGTGCCTA
  GAGAAGGGCAGGAGGAGGATGACGACGATGAGGAAGACGATGCTGACGAGGAGGCTCCCAAGCCCGACCA
  TTTTGTTCAGGACCCTGCAGTGCTGAGAGAGAAGGCAGAAGCCAGGCGCATGGCCTTTCTCGCCAAGAAA
  GGGTACCGGCATGACAGCTCAACAGCAGTGGCCGGCAGCCCCCGAGGCCATGGGCAGAGCCGCGAGACAA
  CCCAGGAACGCAGGAAGAAGGAAGCCAACAAGGCGACAAGAGCCAACCACAACCGGAGAACCATGGCCGA
  CCGCAAGAGGAGCAAAGGCATGATCCCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203391 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPALPLDQLQITHKDPKTGKLRTSPALHPEQKADRYFVLYKPPPKDNIPALVEEYLERATFVANDLDWLL
  ALPHDKFWCQVIFDETLQKCLDSYLRYVPRKFDEGVASAPEVVDMQKRLHRSVFLTFLRMSTHKESKDHF
  ISPSAFGEILYNNFLFDIPKILDLCVLFGKGNSPLLQKMIGNIFTQQPSYYSDLDETLPTILQVFSNILQ
  HCGLQGDGANTTPQKLEERGRLTPSDMPLLELKDIVLYLCDTCTTLWAFLDIFPLACQTFQKHDFCYRLA
  SFYEAAIPEMESAIKKRRLEDSKLLGDLWQRLSHSRKKLMEIFHIILNQICLLPILESSCDNIQGFIEEF
  LQIFSSLLQEKRFLRDYDALFPVAEDISLLQQASSVLDETRTAYILQAVESAWEGVDRRKATDAKDPSVI
  EEPNGEPNGVTVTAEAVSQASSHPENSEEEECMGAAAAVGPAMCGVELDSLISQVKDLLPDLGEGFILAC
  LEYYHYDPEQVINNILEERLAPTLSQLDRNLDREMKPDPTPLLTSRHNVFQNDEFDVFSRDSVDLSRVHK
  GKSTRKEENTRSLLNDKRAVAAQRQRYEQYSVVVEEVPLQPGESLPYHSVYYEDEYDDTYDGNQVGANDA
  DSDDELISRRPFTIPQVLRTKVPREGQEEDDDDEEDDADEEAPKPDHFVQDPAVLREKAEARRMAFLAKK
  GYRHDSSTAVAGSPRGHGQSRETTQERRKKEANKATRANHNRRTMADRKRSKGMIPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032204
• ORF Size: 2271 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032204.5
• RefSeq Size: 2840 bp
• RefSeq ORF: 2274 bp
• Locus ID: 84164
• UniProt ID: Q9H1I8
• Cytogenetics: 22q12.2
• Domains: CUE
• Protein Families: Druggable Genome, Transcription Factors
• MW: 86.4 kDa
• Gene Summary: Plays a role in DNA damage repair as component of the ASCC complex. Recruits ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains (PubMed:29144457). Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation (PubMed:12077347).[UniProtKB/Swiss-Prot Function]