PYCR3 (NM_023078) Human Tagged ORF Clone

CAT#: RC203382

PYCRL (Myc-DDK-tagged)-Human pyrroline-5-carboxylate reductase-like (PYCRL)

Product Images

Circular map for RC203382

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PYCRL (Cat# RC203382, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCRL(Cat# TA502033). Positive lysates LY411510 (100ug) and LC411510 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY411510) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203382 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PYCR3 protein (Cat# TP303382). The protein was produced from HEK293T cells transfected with PYCR3 cDNA clone (Cat# RC203382) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PYCR3
• Synonyms: PYCRL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203382 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTGCGGAGCCGTCTCCGCGGCGCGTGGGCTTCGTGGGCGCGGGCCGCATGGCGGGGGCCATCG
  CGCAGGGCCTCATCAGAGCAGGAAAAGTGGAAGCTCAGCACATACTGGCCAGTGCACCAACAGACAGGAA
  CCTATGTCACTTTCAAGCTCTGGGTTGCCGGACCACGCACTCCAACCAGGAGGTGCTGCAGAGCTGCCTG
  CTCGTCATCTTTGCCACCAAGCCTCATGTGCTGCCAGCTGTCCTGGCAGAGGTGGCTCCTGTGGTCACCA
  CTGAACACATCTTGGTGTCCGTGGCTGCTGGGGTGTCTCTGAGCACCCTGGAGGAGCTGCTGCCCCCAAA
  CACACGGGTGCTGCGGGTCTTGCCCAACCTGCCCTGTGTGGTCCAGGAAGGGGCCATAGTGATGGCGCGG
  GGCCGCCACGTGGGGAGCAGCGAGACCAACCTCCTGCAGCATCTGCTGGAGGCCTGTGGGCGGTGTGAGG
  AGGTGCCTGAAGCCTACGTCGACATCCACACTGGCCTCAGTGGCAGTGGCGTGGCCTTCGTGTGTGCATT
  CTCCGAGGCCCTGGCTGAAGGAGCCGTCAAGATGGGCATGCCCAGCAGCCTGGCCCACCGCATCGCTGCC
  CAGACCCTGCTGGGGACGGCCAAGATGCTGCTGCACGAGGGCCAACACCCAGCCCAGCTGCGCTCAGACG
  TGTGCACCCCGGGTGGCACCACCATCTATGGACTCCACGCCCTGGAGCAGGGCGGGCTGCGAGCAGCCAC
  CATGAGCGCCGTGGAGGCTGCCACCTGCCGGGCCAAGGAGCTCAGCAGAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203382 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAEPSPRRVGFVGAGRMAGAIAQGLIRAGKVEAQHILASAPTDRNLCHFQALGCRTTHSNQEVLQSCL
  LVIFATKPHVLPAVLAEVAPVVTTEHILVSVAAGVSLSTLEELLPPNTRVLRVLPNLPCVVQEGAIVMAR
  GRHVGSSETNLLQHLLEACGRCEEVPEAYVDIHTGLSGSGVAFVCAFSEALAEGAVKMGMPSSLAHRIAA
  QTLLGTAKMLLHEGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAATCRAKELSRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023078
• ORF Size: 822 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023078.1, NP_075566.1
• RefSeq Size: 2678 bp
• RefSeq ORF: 825 bp
• Locus ID: 65263
• UniProt ID: Q53H96
• Cytogenetics: 8q24.3
• Domains: P5CR
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• MW: 28.6 kDa
• Gene Summary: This gene encodes a protein that belongs to the pyrroline-5-carboxylate reductase family of enzymes. Members of this family catalyze the final step in proline biosynthesis, converting pyrroline-5-carboxylate to proline. Glutamate and ornithine are precursors in the synthesis of proline. The protein encoded by this gene is a cytoplasmic enzyme involved in the biosynthesis of proline from ornithine. [provided by RefSeq, Aug 2016]