RUSF1 (NM_022744) Human Tagged ORF Clone

CAT#: RC203381

C16orf58 (Myc-DDK-tagged)-Human chromosome 16 open reading frame 58 (C16orf58)

Product Images

Circular map for RC203381

• 

  Western blot validation of overexpression lysate (Cat# LY411583) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203381 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RUSF1
• Synonyms: C16orf58; RUS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203381 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACGACGCGGGTTTGGAGACCCCGCTGTGTTCCGAGCAGTTCGGCTCCGGGGAGGCACGGGGCT
  GCCGCGCCGCCGCGGACGGGAGCCTGCAGTGGGAGGTCGGGGGCTGGCGCTGGTGGGGGCTCTCCAGGGC
  CTTCACGGTCAAACCTGAAGGACGAGATGCGGGCGAAGTGGGGGCTTCCGGGGCCCCCTCACCGCCCCTC
  TCCGGGCTCCAGGCCGTGTTCCTGCCTCAGGGCTTCCCTGATAGCGTCAGCCCGGACTACTTGCCCTACC
  AGCTGTGGGATTCCGTGCAGGCGTTTGCTTCCAGCCTCTCCGGCTCCCTAGCCACCCAGGCAGTCTTGCT
  GGGCATAGGGGTGGGGAACGCAAAAGCCACTGTTTCAGCTGCCACGGCCACCTGGCTCGTGAAAGATTCA
  ACTGGCATGCTGGGCCGCATCGTCTTTGCCTGGTGGAAAGGGAGCAAACTGGACTGCAATGCCAAGCAGT
  GGAGGCTTTTTGCGGACATCCTCAATGACGTAGCCATGTTCCTTGAGATTATGGCTCCTGTATGCCCAAT
  CTGTTTCACCATGACCGTCTCCACCAGCAACCTAGCCAAGTGCATCGTGAGTGTTGCTGGTGGGGCCACT
  CGGGCTGCCCTGACCGTGCACCAGGCTCGGAGGAACAACATGGCTGACGTGTCAGCCAAGGACAGCAGCC
  AGGAGACGCTGGTGAACCTGGCGGGGCTCTTGGTCAGCCTCCTGATGCTCCCTCTGGTGTCAGGTTGCCC
  TGGCTTCAGCCTTGGATGTTTCTTCTTCCTCACTGCCCTCCACATCTACGCCAACTACCGCGCGGTCCGA
  GCCCTGGTCATGGAGACCTTGAACGAAGGCCGGCTCCGGCTGGTCCTGAAGCACTACCTTCAGAGGGGAG
  AGGTACTCGACCCAACTGCAGCCAATCGCATGGAGCCGCTGTGGACAGGTTTCTGGCCAGCTCCGTCTCT
  ATCCCTGGGGGTCCCCTTACACCGCTTGGTCTCCAGTGTCTTTGAGCTGCAGCAGCTGGTTGAGGGGCAC
  CAAGAATCCTACCTCCTCTGCTGGGACCAGTCACAAAACCAGGTACAGGTAGTTCTGAACCAGAAGGCAG
  GCCCCAAGACCATCCTAAGGGCCGCCACACATGGGCTGATGCTTGGGGCCCTGCAGGGAGATGGACCCCT
  TCCAGCAGAGCTGGAGGAGCTGAGGAACCGGGTGCGGGCAGGTCCTAAGAAAGAGAGCTGGGTCGTCGTC
  AAGGAGACACACGAAGTGTTGGACATGCTGTTCCCAAAGTTCTTGAAAGGACTGCAGGATGCCGGCTGGA
  AGACCGAGAAGCACCAGCTAGAGGTGGATGAGTGGAGGGCCACATGGCTTCTGTCTCCCGAAAAGAAGGT
  CTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203381 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADDAGLETPLCSEQFGSGEARGCRAAADGSLQWEVGGWRWWGLSRAFTVKPEGRDAGEVGASGAPSPPL
  SGLQAVFLPQGFPDSVSPDYLPYQLWDSVQAFASSLSGSLATQAVLLGIGVGNAKATVSAATATWLVKDS
  TGMLGRIVFAWWKGSKLDCNAKQWRLFADILNDVAMFLEIMAPVCPICFTMTVSTSNLAKCIVSVAGGAT
  RAALTVHQARRNNMADVSAKDSSQETLVNLAGLLVSLLMLPLVSGCPGFSLGCFFFLTALHIYANYRAVR
  ALVMETLNEGRLRLVLKHYLQRGEVLDPTAANRMEPLWTGFWPAPSLSLGVPLHRLVSSVFELQQLVEGH
  QESYLLCWDQSQNQVQVVLNQKAGPKTILRAATHGLMLGALQGDGPLPAELEELRNRVRAGPKKESWVVV
  KETHEVLDMLFPKFLKGLQDAGWKTEKHQLEVDEWRATWLLSPEKKVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022744
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022744.4
• RefSeq Size: 2829 bp
• RefSeq ORF: 1407 bp
• Locus ID: 64755
• UniProt ID: Q96GQ5
• Cytogenetics: 16p11.2
• Domains: DUF647
• Protein Families: Transmembrane
• MW: 51 kDa
• Gene Summary: This gene encodes a putative transmembrane protein containing a conserved DUF647 domain that may be involved in protein-protein interaction. The encoded protein is related to a plant protein that participates in ultraviolet B light-sensing during root morphogenesis. [provided by RefSeq, Feb 2013]