TM2D3 (NM_025141) Human Tagged ORF Clone

CAT#: RC203360

TM2D3 (Myc-DDK-tagged)-Human TM2 domain containing 3 (TM2D3), transcript variant 2

Product Images

Circular map for RC203360

• 

  Western blot validation of overexpression lysate (Cat# LY410881) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203360 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TM2D3
• Synonyms: BLP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203360 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGAGGGGTGCGCCCGCTGCGGGGCCTCCGCGCCTTGTGTCGCGTGCTGCTCTTCCTCTCGCAGT
  TCTGCATTCTGTCGGGCGGTGAAAGTACTGAAATCCCACCTTATGTGATGAAGTGTCCGAGCAATGGTTT
  GTGTAGCAGGCTTCCTGCAGACTGTATAGACTGCACAACAAATTTCTCCTGTACCTATGGGAAGCCTGTC
  ACTTTTGACTGTGCAGTGAAACCATCTGTTACCTGTGTTGATCAAGACTTCAAATCCCAAAAGAACTTCA
  TCATTAACATGACTTGCAGATTTTGCTGGCAGCTTCCTGAAACAGATTACGAGTGTACCAACTCCACCAG
  CTGCATGACGGTGTCCTGTCCTCGGCAGCGCTACCCTGCCAACTGCACGGTGCGGGACCACGTCCACTGC
  TTGGGTAACCGTACTTTTCCCAAAATGCTATATTGCAATTGGACTGGAGGCTATAAGTGGTCTACGGCTC
  TGGCTCTAAGCATCACCCTCGGTGGGTTTGGAGCAGACCGTTTCTACCTGGGCCAGTGGCGGGAAGGCCT
  CGGCAAGCTCTTCAGCTTCGGTGGCCTGGGAATATGGACGCTGATAGACGTCCTGCTCATTGGAGTTGGC
  TATGTTGGACCAGCAGATGGCTCTTTGTACATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203360 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGGVRPLRGLRALCRVLLFLSQFCILSGGESTEIPPYVMKCPSNGLCSRLPADCIDCTTNFSCTYGKPV
  TFDCAVKPSVTCVDQDFKSQKNFIINMTCRFCWQLPETDYECTNSTSCMTVSCPRQRYPANCTVRDHVHC
  LGNRTFPKMLYCNWTGGYKWSTALALSITLGGFGADRFYLGQWREGLGKLFSFGGLGIWTLIDVLLIGVG
  YVGPADGSLYI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025141
• ORF Size: 663 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025141.3
• RefSeq Size: 1343 bp
• RefSeq ORF: 666 bp
• Locus ID: 80213
• UniProt ID: Q9BRN9
• Cytogenetics: 15q26.3
• Domains: TM2
• Protein Families: Druggable Genome, Transmembrane
• MW: 24.4 kDa
• Gene Summary: The protein encoded by this gene contains a structural module related to that of the seven transmembrane domain G protein-coupled receptor superfamily. This protein has sequence and structural similarities to the beta-amyloid binding protein (BBP), but, unlike BBP, it does not regulate a response to beta-amyloid peptide. This protein may have regulatory roles in cell death or proliferation signal cascades. Several alternatively spliced transcript variants of this gene are described but the full length nature of some variants has not been determined. Multiple polyadenylation sites have been found in this gene. [provided by RefSeq, Jul 2008]