CIAO2A (NM_032231) Human Tagged ORF Clone

CAT#: RC203357

FAM96A (Myc-DDK-tagged)-Human family with sequence similarity 96, member A (FAM96A), transcript variant 1

Product Images

Circular map for RC203357

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  Western blot validation of overexpression lysate (Cat# LY410258) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203357 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CIAO2A protein (Cat# TP303357). The protein was produced from HEK293T cells transfected with CIAO2A cDNA clone (Cat# RC203357) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CIAO2A
• Synonyms: CIA2A; FAM96A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203357 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGGGTGTCCGGGCTGCTCTCCTGGACGCTGAGCAGAGTCCTGTGGCTCTCCGGCCTCTCTGAGC
  CGGGAGCTGCCCGGCAGCCCCGGATCATGGAAGAGAAAGCGCTAGAGGTTTATGATTTGATTAGAACTAT
  CCGGGACCCAGAAAAGCCCAATACTTTAGAAGAACTGGAAGTGGTCTCGGAAAGTTGTGTGGAAGTTCAG
  GAGATAAATGAAGAAGAATATCTGGTTATTATCAGGTTCACGCCAACAGTACCTCATTGCTCTTTGGCGA
  CTCTTATTGGGCTGTGCTTAAGAGTAAAACTTCAGCGATGTTTACCATTTAAACATAAGTTGGAAATCTA
  CATTTCTGAAGGAACCCACTCAACAGAAGAAGACATCAATAAGCAGATAAATGACAAAGAGCGAGTGGCA
  GCTGCAATGGAAAACCCCAACTTACGGGAAATTGTGGAACAGTGTGTCCTTGAACCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203357 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQRVSGLLSWTLSRVLWLSGLSEPGAARQPRIMEEKALEVYDLIRTIRDPEKPNTLEELEVVSESCVEVQ
  EINEEEYLVIIRFTPTVPHCSLATLIGLCLRVKLQRCLPFKHKLEIYISEGTHSTEEDINKQINDKERVA
  AAMENPNLREIVEQCVLEPD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032231
• ORF Size: 480 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032231.3
• RefSeq Size: 1114 bp
• RefSeq ORF: 483 bp
• Locus ID: 84191
• UniProt ID: Q9H5X1
• Cytogenetics: 15q22.31
• Domains: DUF59
• MW: 18.4 kDa
• Gene Summary: Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed:23891004). As a CIA complex component and in collaboration with CIAO1 specifically matures ACO1 and stabilizes IREB2, connecting cytosolic iron-sulfur protein maturation with cellular iron regulation (PubMed:23891004). May play a role in chromosome segregation through establishment of sister chromatid cohesion. May induce apoptosis in collaboration with APAF1 (PubMed:25716227).[UniProtKB/Swiss-Prot Function]