Density Regulated Protein (DENR) (NM_003677) Human Tagged ORF Clone

CAT#: RC203227

DENR (Myc-DDK-tagged)-Human density-regulated protein (DENR)

Product Images

Circular map for RC203227

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  Western blot validation of overexpression lysate (Cat# LY418507) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203227 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DENR protein (Cat# TP303227). The protein was produced from HEK293T cells transfected with DENR cDNA clone (Cat# RC203227) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Density Regulated Protein
• Synonyms: DRP; DRP1; SMAP-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203227 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGACATTTCTGAATCCAGCGGGGCTGACTGCAAAGGAGACCCAAGGAACAGTGCCAAGTTAG
  ATGCCGATTACCCACTTCGAGTCCTTTATTGTGGAGTCTGTTCATTACCAACAGAGTACTGTGAATATAT
  GCCTGATGTTGCTAAATGTAGACAATGGTTAGAGAAGAATTTTCCAAATGAATTTGCAAAACTTACTGTA
  GAAAATTCACCCAAACAAGAAGCTGGAATTAGTGAGGGTCAAGGAACAGCAGGGGAAGAAGAGGAGAAGA
  AAAAACAGAAGAGAGGTGGAAGGGGTCAAATAAAACAAAAAAAGAAGACCGTACCACAAAAGGTTACTAT
  AGCCAAAATTCCCAGAGCAAAGAAGAAATATGTGACAAGAGTATGTGGCCTTGCAACTTTTGAAATTGAT
  CTTAAAGAAGCACAAAGATTTTTTGCTCAAAAATTCTCCTGTGGTGCCTCAGTAACAGGGGAGGATGAAA
  TTATCATTCAGGGAGATTTTACAGATGACATAATTGATGTCATTCAGGAAAAATGGCCAGAGGTAGATGA
  TGACAGCATCGAAGATCTTGGAGAAGTAAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203227 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAADISESSGADCKGDPRNSAKLDADYPLRVLYCGVCSLPTEYCEYMPDVAKCRQWLEKNFPNEFAKLTV
  ENSPKQEAGISEGQGTAGEEEEKKKQKRGGRGQIKQKKKTVPQKVTIAKIPRAKKKYVTRVCGLATFEID
  LKEAQRFFAQKFSCGASVTGEDEIIIQGDFTDDIIDVIQEKWPEVDDDSIEDLGEVKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003677
• ORF Size: 594 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003677.5
• RefSeq Size: 3061 bp
• RefSeq ORF: 597 bp
• Locus ID: 8562
• UniProt ID: O43583
• Cytogenetics: 12q24.31
• MW: 22.1 kDa
• Gene Summary: This gene encodes a protein whose expression was found to increase in cultured cells at high density but not during growth arrest. This gene was also shown to have increased expression in cells overexpressing HER-2/neu proto-oncogene. The protein contains an SUI1 domain. In budding yeast, SUI1 is a translation initiation factor that along with eIF-2 and the initiator tRNA-Met, directs the ribosome to the proper translation start site. Proteins similar to SUI have been found in mammals, insects, and plants. [provided by RefSeq, Jul 2008]