SCLY (NM_016510) Human Tagged ORF Clone

CAT#: RC203164

SCLY (Myc-DDK-tagged)-Human selenocysteine lyase (SCLY)

Product Images

Circular map for RC203164

• 

  Western blot validation of overexpression lysate (Cat# LY413947) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203164 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCLY
• Synonyms: hSCL; SCL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203164 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGGCCGTGGCGCCGGGGAGGGATGCGCCGGCACCCGCGGCGAGTCAGCCCAGCGGCTGCGGGA
  AACACAACTCGCCGGAGAGGAAAGTTTATATGGACTATAATGCAACGACTCCCCTGGAGCCAGAAGTTAT
  CCAGGCCATGACCAAGGCCATGTGGGAAGCCTGGGGAAATCCCAGCAGCCCGTATTCAGCAGGAAGAAAG
  GCCAAGGATATTATAAATGCAGCTCGGGAAAGCCTCGCGAAGATGATAGGGGGGAAACCTCAAGATATAA
  TCTTCACTTCCGGGGGCACTGAGTCAAATAATTTAGTAATCCATTCTGTGGTGAAACATTTCCACGCAAA
  CCAGACCTCAAAGGGACACACAGGTGGGCACCACAGCCCAGTGAAGGGGGCCAAGCCCCATTTCATTACT
  TCCTCGGTGGAACACGACTCCATCCGGCTGCCCCTGGAGCACCTGGTGGAAGAACAAGTGGCAGCGGTCA
  CCTTTGTCCCGGTGTCCAAGGTGAGCGGGCAGACAGAGGTGGACGACATCCTCGCGGCAGTCCGCCCGAC
  CACACGCCTCGTGACCATCATGCTGGCCAACAATGAGACTGGCATTGTCATGCCTGTCCCTGAAATCAGT
  CAGCGCATTAAAGCCCTGAACCAGGAACGGGTGGCAGCTGGGCTACCTCCCATCCTCGTGCACACGGATG
  CTGCACAGGCCTTGGGGAAGCAGCGCGTGGATGTGGAGGACCTGGGCGTGGACTTCCTTACAATCGTGGG
  GCACAAGTTTTATGGTCCCAGGATTGGCGCACTTTATATACGAGGACTTGGTGAATTTACCCCTCTCTAC
  CCTATGCTATTTGGAGGTGGACAAGAACGGAATTTCAGGCCAGGGACAGAGAACACCCCAATGATTGCTG
  GCCTTGGGAAGGCCGCGGAGCTGGTGACCCAGAACTGCGAGGCTTATGAGGCCCACATGAGGGACGTCCG
  CGACTACCTGGAAGAGAGGCTGGAAGCTGAATTCGGTCAGAAGAGAATCCATCTGAATAGCCAGTTTCCA
  GGCACCCAGCGGCTTCCCAATACCTGTAACTTTTCCATCCGGGGACCCCGGCTTCAAGGCCACGTGGTGC
  TTGCGCAGTGCCGAGTGCTGATGGCCAGTGTGGGGGCCGCGTGCCACTCGGACCACGGGGACCAGCCGTC
  CCCAGTGCTGCTGAGCTACGGTGTCCCCTTCGACGTGGCCAGGAACGCGCTCCGGCTCAGCGTGGGCCGC
  AGCACCACCAGGGCCGAGGTGGACCTCGTCGTGCAGGACCTGAAGCAGGCCGTGGCGCAGCTGGAGGACC
  AGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203164 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEAAVAPGRDAPAPAASQPSGCGKHNSPERKVYMDYNATTPLEPEVIQAMTKAMWEAWGNPSSPYSAGRK
  AKDIINAARESLAKMIGGKPQDIIFTSGGTESNNLVIHSVVKHFHANQTSKGHTGGHHSPVKGAKPHFIT
  SSVEHDSIRLPLEHLVEEQVAAVTFVPVSKVSGQTEVDDILAAVRPTTRLVTIMLANNETGIVMPVPEIS
  QRIKALNQERVAAGLPPILVHTDAAQALGKQRVDVEDLGVDFLTIVGHKFYGPRIGALYIRGLGEFTPLY
  PMLFGGGQERNFRPGTENTPMIAGLGKAAELVTQNCEAYEAHMRDVRDYLEERLEAEFGQKRIHLNSQFP
  GTQRLPNTCNFSIRGPRLQGHVVLAQCRVLMASVGAACHSDHGDQPSPVLLSYGVPFDVARNALRLSVGR
  STTRAEVDLVVQDLKQAVAQLEDQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016510
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016510.3, NP_057594.2
• RefSeq Size: 2544 bp
• RefSeq ORF: 1338 bp
• Locus ID: 51540
• UniProt ID: Q96I15
• Cytogenetics: 2q37.3
• Domains: aminotran_5
• Protein Pathways: Metabolic pathways, Selenoamino acid metabolism
• MW: 48.2 kDa
• Gene Summary: Selenocysteine lyase (SCLY; EC 4.4.1.16) catalyzes the pyridoxal 5-prime phosphate-dependent conversion of L-selenocysteine to L-alanine and elemental selenium (Mihara et al., 2000 [PubMed 10692412]).[supplied by OMIM, Mar 2008]