PSMD7 (NM_002811) Human Tagged ORF Clone

CAT#: RC203133

PSMD7 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (PSMD7)

Product Images

Western blot validation of overexpression lysate (Cat# LY419091) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203133 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PSMD7 protein (Cat# TP303133). The protein was produced from HEK293T cells transfected with PSMD7 cDNA clone (Cat# RC203133) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PSMD7
• Synonyms: MOV34; P40; Rpn8; S12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203133 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGAGCTGGCAGTGCAGAAGGTGGTGGTCCACCCCCTGGTGCTGCTCAGTGTGGTGGATCATTTCA
  ACCGAATCGGCAAGGTTGGAAACCAGAAGCGTGTTGTTGGTGTGCTTTTGGGGTCATGGCAAAAGAAAGT
  ACTTGATGTATCGAACAGTTTTGCAGTTCCTTTTGATGAAGATGACAAAGACGATTCTGTATGGTTTTTA
  GACCATGATTATTTGGAAAACATGTATGGAATGTTTAAGAAAGTCAATGCCAGGGAAAGAATAGTTGGCT
  GGTACCACACAGGCCCTAAACTACACAAGAATGACATTGCCATCAACGAACTCATGAAAAGATACTGTCC
  TAATTCCGTATTGGTCATCATTGATGTGAAGCCGAAGGACCTAGGGCTGCCTACAGAAGCGTACATTTCA
  GTGGAAGAAGTCCATGATGATGGAACTCCAACCTCGAAAACATTTGAACACGTGACCAGTGAAATTGGAG
  CAGAGGAAGCTGAGGAAGTTGGAGTTGAACACTTGTTACGAGATATCAAAGACACGACGGTGGGCACTCT
  GTCCCAGCGGATCACAAACCAGGTCCATGGTTTGAAGGGACTGAACTCCAAGCTTCTGGATATCAGGAGC
  TACCTGGAAAAAGTCGCCACAGGCAAGCTGCCCATCAACCACCAGATCATCTACCAGCTGCAGGACGTCT
  TCAACCTGCTGCCAGATGTCAGCCTGCAGGAGTTCGTCAAGGCCTTTTACCTGAAGACCAATGACCAGAT
  GGTGGTAGTGTACTTGGCCTCGCTGATCCGTTCCGTGGTCGCCCTGCACAACCTCATCAACAACAAGATT
  GCCAACCGGGATGCAGAGAAGAAAGAAGGGCAGGAGAAAGAAGAGAGCAAAAAGGATAGGAAAGAGGACA
  AGGAGAAAGATAAAGATAAGGAAAAGAGTGATGTAAAGAAAGAGGAGAAAAAGGAGAAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203133 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPELAVQKVVVHPLVLLSVVDHFNRIGKVGNQKRVVGVLLGSWQKKVLDVSNSFAVPFDEDDKDDSVWFL
  DHDYLENMYGMFKKVNARERIVGWYHTGPKLHKNDIAINELMKRYCPNSVLVIIDVKPKDLGLPTEAYIS
  VEEVHDDGTPTSKTFEHVTSEIGAEEAEEVGVEHLLRDIKDTTVGTLSQRITNQVHGLKGLNSKLLDIRS
  YLEKVATGKLPINHQIIYQLQDVFNLLPDVSLQEFVKAFYLKTNDQMVVVYLASLIRSVVALHNLINNKI
  ANRDAEKKEGQEKEESKKDRKEDKEKDKDKEKSDVKKEEKKEKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002811
• ORF Size: 972 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002811.5
• RefSeq Size: 1686 bp
• RefSeq ORF: 975 bp
• Locus ID: 5713
• UniProt ID: P51665
• Cytogenetics: 16q23.1
• Domains: JAB_MPN
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• MW: 37 kDa
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17. [provided by RefSeq, Jul 2008]