Coilin (COIL) (NM_004645) Human Tagged ORF Clone

CAT#: RC203119

COIL (Myc-DDK-tagged)-Human coilin (COIL)

Product Images

Circular map for RC203119

• 

  Western blot validation of overexpression lysate (Cat# LY417847) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203119 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Coilin
• Synonyms: CLN80; p80-coilin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203119 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTTCCGAGACGGTTAGGCTACGGCTTCAATTTGATTACCCGCCGCCAGCTACCCCGCACTGTA
  CGGCCTTCTGGCTTCTGGTCGACTTGAACAGATGCCGAGTCGTCACAGATCTCATTAGTCTCATCCGCCA
  GCGCTTCGGCTTCAGTTCTGGGGCCTTCCTAGGCCTCTACCTGGAGGGGGGGCTCTTGCCCCCCGCCGAG
  AGCGCGCGCCTTGTGAGAGACAACGACTGCCTCAGAGTTAAATTAGAAGAGAGAGGAGTTGCTGAGAATT
  CTGTAGTCATCAGTAATGGTGACATTAATTTATCTCTTAGAAAAGCAAAGAAGCGGGCATTTCAGTTAGA
  GGAGGGTGAAGAAACTGAACCAGATTGCAAATATTCAAAGAAGCATTGGAAGAGTCGAGAGAACAATAAC
  AATAATGAGAAGGTCTTGGATCTGGAACCAAAAGCTGTCACAGATCAGACTGTCAGCAAAAAAAACAAGA
  GAAAAAATAAAGCAACCTGTGGCACAGTGGGTGATGATAACGAAGAGGCCAAAAGAAAATCACCAAAGAA
  AAAGGAGAAATGTGAATATAAAAAAAAGGCTAAGAATCCCAAGTCTCCGAAAGTACAGGCAGTGAAAGAC
  TGGGCCAATCAGAGATGTAGTTCTCCAAAAGGTTCTGCTAGAAACAGCCTTGTTAAAGCCAAAAGGAAAG
  GTAGTGTAAGCGTTTGCTCAAAAGAGAGTCCCAGTTCCTCCTCGGAGTCTGAATCTTGTGATGAATCTAT
  CAGTGATGGTCCCAGCAAAGTCACTTTGGAGGCCAGAAATTCCTCAGAGAAATTACCAACTGAGTTATCA
  AAGGAAGAACCCTCTACCAAAAATACAACTGCAGACAAACTGGCTATAAAACTTGGCTTTAGCCTTACCC
  CCAGCAAGGGCAAGACCTCTGGAACAACATCTTCCAGTTCAGACTCTAGTGCAGAGTCAGACGACCAATG
  CTTGATGTCATCGAGCACCCCGGAGTGTGCTGCGGGTTTCTTAAAGACAGTAGGCCTTTTTGCAGGAAGA
  GGTCGTCCAGGCCCAGGGCTGTCATCACAGACTGCAGGTGCTGCTGGATGGAGGCGTTCTGGCTCAAATG
  GTGGTGGACAGGCTCCTGGTGCTTCTCCCAGTGTGTCTCTCCCTGCTAGTTTAGGAAGAGGATGGGGTAG
  AGAAGAGAACCTTTTTTCTTGGAAGGGAGCTAAGGGACGGGGCATGCGGGGGAGAGGTCGAGGACGAGGG
  CATCCTGTTTCCTGTGTTGTAAATAGAAGCACTGACAACCAGAGGCAACAGCAATTAAATGACGTGGTAA
  AAAATTCATCTACTATTATCCAGAATCCAGTAGAGACACCCAAGAAGGACTATAGTCTGTTACCACTGTT
  AGCAGCTGCCCCTCAAGTTGGAGAAAAGATTGCATTTAAGCTTTTGGAGCTAACATCCAGTTACTCTCCT
  GATGTCTCTGACTACAAGGAAGGAAGAATATTAAGCCACAATCCAGAGACCCAGCAAGTAGATATAGAAA
  TTCTTTCATCCTTACCTGCCTTGAGAGAACCTGGGAAATTTGATTTAGTTTATCACAATGAAAATGGAGC
  CGAGGTAGTGGAGTACGCTGTGACACAGGAGAGCAAGATCACTGTATTTTGGAAAGAGTTGATTGACCCA
  AGACTGATTATTGAATCTCCAAGTAACACATCAAGTACAGAACCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203119 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAASETVRLRLQFDYPPPATPHCTAFWLLVDLNRCRVVTDLISLIRQRFGFSSGAFLGLYLEGGLLPPAE
  SARLVRDNDCLRVKLEERGVAENSVVISNGDINLSLRKAKKRAFQLEEGEETEPDCKYSKKHWKSRENNN
  NNEKVLDLEPKAVTDQTVSKKNKRKNKATCGTVGDDNEEAKRKSPKKKEKCEYKKKAKNPKSPKVQAVKD
  WANQRCSSPKGSARNSLVKAKRKGSVSVCSKESPSSSSESESCDESISDGPSKVTLEARNSSEKLPTELS
  KEEPSTKNTTADKLAIKLGFSLTPSKGKTSGTTSSSSDSSAESDDQCLMSSSTPECAAGFLKTVGLFAGR
  GRPGPGLSSQTAGAAGWRRSGSNGGGQAPGASPSVSLPASLGRGWGREENLFSWKGAKGRGMRGRGRGRG
  HPVSCVVNRSTDNQRQQQLNDVVKNSSTIIQNPVETPKKDYSLLPLLAAAPQVGEKIAFKLLELTSSYSP
  DVSDYKEGRILSHNPETQQVDIEILSSLPALREPGKFDLVYHNENGAEVVEYAVTQESKITVFWKELIDP
  RLIIESPSNTSSTEPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004645
• ORF Size: 1728 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004645.3
• RefSeq Size: 2649 bp
• RefSeq ORF: 1731 bp
• Locus ID: 8161
• UniProt ID: P38432
• Cytogenetics: 17q22
• Protein Families: Stem cell - Pluripotency
• MW: 62.6 kDa
• Gene Summary: The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. [provided by RefSeq, Jul 2008]