ELK3 (NM_005230) Human Tagged ORF Clone

CAT#: RC203114

ELK3 (Myc-DDK-tagged)-Human ELK3, ETS-domain protein (SRF accessory protein 2) (ELK3)

Product Images

Circular map for RC203114

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ELK3 (Cat# RC203114, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELK3(Cat# TA503603). Positive lysates LY401602 (100ug) and LC401602 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401602) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203114 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ELK3 protein (Cat# TP303114). The protein was produced from HEK293T cells transfected with ELK3 cDNA clone (Cat# RC203114) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ELK3
• Synonyms: ERP; NET; SAP-2; SAP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203114 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGTGCAATCACGCTGTGGCAGTTCCTGTTGCAGTTGCTGCTGGATCAGAAACATGAGCATTTGA
  TCTGCTGGACCTCGAACGATGGTGAATTCAAGCTCCTCAAAGCAGAAGAAGTGGCCAAGCTGTGGGGACT
  CCGAAAAAACAAAACAAATATGAACTATGATAAGCTGAGCAGAGCCCTGCGATACTATTATGACAAGAAC
  ATCATCAAGAAGGTGATCGGGCAGAAGTTTGTGTACAAGTTTGTCTCTTTCCCGGAGATCCTGAAGATGG
  ATCCTCACGCGGTGGAGATCAGCCGGGAGAGCCTTCTGCTGCAGGACAGCGACTGCAAGGCGTCTCCGGA
  GGGCCGCGAGGCCCACAAACACGGCCTGGCCGCCCTCAGAAGCACGAGCCGCAACGAATACATCCACTCA
  GGCCTGTACTCGTCCTTCACCATTAATTCCCTGCAGAACCCACCAGACGCCTTCAAGGCCATCAAGACGG
  AGAAGCTGGAGGAGCCGCCCGAAGACAGCCCCCCCGTGGAAGAAGTCAGGACTGTGATCAGGTTTGTGAC
  CAATAAAACCGACAAGCACGTCACCAGGCCGGTGGTGTCCCTGCCTTCCACGTCAGAGGCTGCGGCGGCG
  TCCGCCTTCCTGGCCTCGTCCGTCTCGGCCAAGATCTCCTCTTTAATGTTGCCAAACGCTGCCAGTATTT
  CATCCGCCTCACCCTTCTCATCTCGGTCCCCGTCCCTGTCCCCCAACTCACCCCTCCCTTCTGAACACAG
  AAGCCTCTTCCTGGAGGCCGCCTGCCATGACTCCGATTCCCTGGAGCCCTTGAACCTGTCATCGGGCTCC
  AAGACCAAGTCTCCATCTCTTCCCCCAAAGGCCAAAAAACCCAAAGGCTTGGAAATCTCAGCGCCCCCGC
  TGGTGCTCTCCGGCACCGACATCGGCTCCATCGCCCTCAACAGCCCAGCCCTCCCCTCGGGATCCCTCAC
  CCCAGCCTTCTTCACCGCACAGACACCAAATGGATTGCTTCTGACTCCGAGTCCACTGCTCTCCAGCATA
  CATTTCTGGAGCAGCCTTAGTCCAGTTGCTCCGCTGAGTCCTGCCAGGCTGCAAGGGCCAAGCACGCTGT
  TCCAGTTCCCCACACTGCTTAATGGCCACATGCCAGTGCCAATCCCCAGTCTGGACAGAGCTGCTTCTCC
  AGTACTGCTTTCTTCAAACTCTCAGAAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203114 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESAITLWQFLLQLLLDQKHEHLICWTSNDGEFKLLKAEEVAKLWGLRKNKTNMNYDKLSRALRYYYDKN
  IIKKVIGQKFVYKFVSFPEILKMDPHAVEISRESLLLQDSDCKASPEGREAHKHGLAALRSTSRNEYIHS
  GLYSSFTINSLQNPPDAFKAIKTEKLEEPPEDSPPVEEVRTVIRFVTNKTDKHVTRPVVSLPSTSEAAAA
  SAFLASSVSAKISSLMLPNAASISSASPFSSRSPSLSPNSPLPSEHRSLFLEAACHDSDSLEPLNLSSGS
  KTKSPSLPPKAKKPKGLEISAPPLVLSGTDIGSIALNSPALPSGSLTPAFFTAQTPNGLLLTPSPLLSSI
  HFWSSLSPVAPLSPARLQGPSTLFQFPTLLNGHMPVPIPSLDRAASPVLLSSNSQKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005230
• ORF Size: 1221 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005230.4
• RefSeq Size: 2180 bp
• RefSeq ORF: 1224 bp
• Locus ID: 2004
• UniProt ID: P41970
• Cytogenetics: 12q23.1
• Domains: ETS
• Protein Families: Transcription Factors
• MW: 44.2 kDa
• Gene Summary: This gene encodes a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]