PGAP4 (NM_032342) Human Tagged ORF Clone

CAT#: RC202969

TMEM246 (Myc-DDK-tagged)-Human chromosome 9 open reading frame 125 (C9orf125)

Product Images

Circular map for RC202969

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  Western blot validation of overexpression lysate (Cat# LY410179) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202969 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TMEM246 protein (Cat# TP302969). The protein was produced from HEK293T cells transfected with TMEM246 cDNA clone (Cat# RC202969) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PGAP4
• Synonyms: C9orf125; TMEM246
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202969 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACTTCAACCTCTCCAGCTGCCATGCTCCTCCGGAGGCTGCGGCGACTGTCCTGGGGCAGCACTG
  CTGTCCAGCTCTTCATCCTAACAGTGGTGACGTTTGGCCTGCTGGCCCCCCTGGCCTGTCACCGACTTCT
  ACACTCTTACTTCTATCTGCGCCATTGGCATCTGAACCAAATGAGCCAAGAGTTCCTGCAGCAAAGCTTG
  AAAGAGGGTGAGGCTGCCCTCCACTATTTTGAGGAGCTTCCCTCTGCCAATGGCTCAGTGCCCATTGTCT
  GGCAGGCCACCCCCCGGCCCTGGCTGGTGATCACCATCATCACTGTGGACAGGCAGCCTGGCTTCCACTA
  CGTCCTGCAGGTTGTGTCCCAGTTCCACCGGCTTCTTCAGCAATGTGGCCCCCAGTGCGAGGGGCACCAA
  CTCTTCCTGTGCAACGTGGAGCGTAGTGTGAGCCATTTTGATGCCAAGTTGCTCTCCAAGTATGTCCCTG
  TGGCCAATCGCTATGAGGGCACTGAGGATGATTATGGTGATGACCCTTCGACCAACTCGTTTGAGAAAGA
  GAAGCAGGACTATGTCTATTGCCTGGAGTCATCCCTGCAGACCTACAACCCAGACTACGTCCTGATGGTA
  GAAGACGATGCTGTACCAGAAGAGCAGATCTTCCCAGTCTTGGAGCACCTTCTGCGGGCTCGCTTCTCTG
  AGCCACATCTCAGAGATGCCCTTTATCTCAAGCTGTATCACCCCGAGAGGCTCCAGCACTACATCAATCC
  AGAGCCCATGCGGATCCTGGAATGGGTTGGTGTAGGCATGTTGCTGGGGCCCTTACTAACCTGGATATAC
  ATGAGGTTTGCCAGCCGCCCAGGGTTTAGCTGGCCTGTAATGCTCTTCTTCTCCCTGTATAGCATGGGTC
  TGGTGGAGCTGGTGGGTCGGCACTATTTCCTGGAACTGCGGCGGCTGAGTCCTTCCCTGTACAGTGTGGT
  TCCTGCCTCTCAGTGTTGCACCCCAGCCATGCTCTTCCCGGCACCTGCGGCCCGCCGGACCCTCACCTAC
  CTGTCCCAAGTGTACTGCCACAAGGGCTTTGGCAAGGACATGGCACTGTACTCGCTGTTGAGGGCCAAGG
  GAGAGAGGGCCTATGTAGTGGAGCCGAACCTCGTGAAACACATCGGGCTCTTCTCCAGTCTCCGGTACAA
  CTTTCATCCCAGTCTCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202969 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTSTSPAAMLLRRLRRLSWGSTAVQLFILTVVTFGLLAPLACHRLLHSYFYLRHWHLNQMSQEFLQQSL
  KEGEAALHYFEELPSANGSVPIVWQATPRPWLVITIITVDRQPGFHYVLQVVSQFHRLLQQCGPQCEGHQ
  LFLCNVERSVSHFDAKLLSKYVPVANRYEGTEDDYGDDPSTNSFEKEKQDYVYCLESSLQTYNPDYVLMV
  EDDAVPEEQIFPVLEHLLRARFSEPHLRDALYLKLYHPERLQHYINPEPMRILEWVGVGMLLGPLLTWIY
  MRFASRPGFSWPVMLFFSLYSMGLVELVGRHYFLELRRLSPSLYSVVPASQCCTPAMLFPAPAARRTLTY
  LSQVYCHKGFGKDMALYSLLRAKGERAYVVEPNLVKHIGLFSSLRYNFHPSLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032342
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032342.1, NP_115718.1
• RefSeq Size: 2110 bp
• RefSeq ORF: 1212 bp
• Locus ID: 84302
• UniProt ID: Q9BRR3
• Cytogenetics: 9q31.1
• Protein Families: Transmembrane
• MW: 46.6 kDa
• Gene Summary: Golgi-resident glycosylphosphatidylinositol (GPI)-N-acetylgalactosamine transferase involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins (PubMed:29374258). Required for the initial step of GPI-GalNAc biosynthesis, transfers GalNAc to GPI in the Golgi after fatty acid remodeling by PGAP2 (PubMed:29374258).[UniProtKB/Swiss-Prot Function]