DYRK2 (NM_006482) Human Tagged ORF Clone

CAT#: RC202864

DYRK2 (Myc-DDK-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript variant 2

Product Images

Circular map for RC202864

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DYRK2 (Cat# RC202864, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DYRK2 (Cat# TA810286)(1:2000).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DYRK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202864 representing NM_006482
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAACCAGGAAACCTTCGGCCGCCGCTCCCGCCGCCTACCCGACCGGCCGAGGTGGGGACAGCGCCG
  TTCGTCAGCTTCAGGCTTCCCCGGGGCTCGGTGCAGGGGCCACCCGGAGCGGAGTGGGGACTGGCCCGCC
  CTCCCCCATCGCCCTGCCGCCTCTCCGGGCCAGCAACGCTGCCGCCGCAGCCCACACGATTGGCGGCAGT
  AAGCACACAATGAATGATCACCTGCATGTCGGCAGCCACGCTCACGGACAGATCCAGGTTCAACAGTTGT
  TTGAGGATAACAGTAACAAGCGGACAGTGCTCACGACACAACCAAATGGGCTTACAACAGTGGGCAAAAC
  GGGCTTGCCAGTGGTGCCAGAGCGGCAGCTGGACAGCATTCATAGACGGCAGGGGAGCTCCACCTCTCTA
  AAGTCCATGGAAGGCATGGGGAAGGTGAAAGCCACCCCCATGACACCTGAACAAGCAATGAAGCAATACA
  TGCAAAAACTCACAGCCTTCGAACACCATGAGATTTTCAGCTACCCTGAAATATATTTCTTGGGTCTAAA
  TGCTAAGAAGCGCCAGGGCATGACAGGTGGGCCCAACAATGGTGGCTATGATGATGACCAGGGATCATAT
  GTGCAGGTGCCCCACGATCACGTGGCTTACAGGTATGAGGTCCTCAAGGTCATTGGGAAGGGGAGCTTTG
  GGCAGGTGGTCAAGGCCTACGATCACAAAGTCCACCAGCACGTGGCCCTAAAGATGGTGCGGAATGAGAA
  GCGCTTCCACCGGCAAGCAGCGGAGGAGATCCGAATCCTGGAACACCTGCGGAAGCAGGACAAGGATAAC
  ACAATGAATGTCATCCATATGCTGGAGAATTTCACCTTCCGCAACCACATCTGCATGACGTTTGAGCTGC
  TGAGCATGAACCTCTATGAGCTCATCAAGAAGAATAAATTCCAGGGCTTCAGTCTGCCTTTGGTTCGCAA
  GTTTGCCCACTCGATTCTGCAGTGCTTGGATGCTTTGCACAAAAACAGAATAATTCACTGTGACCTTAAG
  CCCGAGAACATTTTGTTAAAGCAGCAGGGTAGAAGCGGTATTAAAGTAATTGATTTTGGCTCCAGTTGTT
  ACGAGCATCAGCGTGTCTACACGTACATCCAGTCGCGTTTTTACCGGGCTCCAGAAGTGATCCTTGGGGC
  CAGGTATGGCATGCCCATTGATATGTGGAGCCTGGGCTGCATTTTAGCAGAGCTCCTGACGGGTTACCCC
  CTCTTGCCTGGGGAAGATGAAGGGGACCAGCTGGCCTGTATGATTGAACTGTTGGGCATGCCCTCACAGA
  AACTGCTGGATGCATCCAAACGAGCCAAAAATTTTGTGAGCTCCAAGGGTTATCCCCGTTACTGCACTGT
  CACGACTCTCTCAGATGGCTCTGTGGTCCTAAACGGAGGCCGTTCCCGGAGGGGGAAACTGAGGGGCCCA
  CCGGAGAGCAGAGAGTGGGGGAACGCGCTGAAGGGGTGTGATGATCCCCTTTTCCTTGACTTCTTAAAAC
  AGTGTTTAGAGTGGGATCCTGCAGTGCGCATGACCCCAGGCCAGGCTTTGCGGCACCCCTGGCTGAGGAG
  GCGGTTGCCAAAGCCTCCCACCGGGGAGAAAACGTCAGTGAAAAGGATAACTGAGAGCACCGGTGCTATC
  ACATCTATATCCAAGTTACCTCCACCTTCTAGCTCAGCTTCCAAACTGAGGACTAATTTGGCGCAGATGA
  CAGATGCCAATGGGAATATTCAGCAGAGGACAGTGTTGCCAAAACTTGTTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202864 representing NM_006482
  Red=Cloning site Green=Tags(s)
  
  MLTRKPSAAAPAAYPTGRGGDSAVRQLQASPGLGAGATRSGVGTGPPSPIALPPLRASNAAAAAHTIGGS
  KHTMNDHLHVGSHAHGQIQVQQLFEDNSNKRTVLTTQPNGLTTVGKTGLPVVPERQLDSIHRRQGSSTSL
  KSMEGMGKVKATPMTPEQAMKQYMQKLTAFEHHEIFSYPEIYFLGLNAKKRQGMTGGPNNGGYDDDQGSY
  VQVPHDHVAYRYEVLKVIGKGSFGQVVKAYDHKVHQHVALKMVRNEKRFHRQAAEEIRILEHLRKQDKDN
  TMNVIHMLENFTFRNHICMTFELLSMNLYELIKKNKFQGFSLPLVRKFAHSILQCLDALHKNRIIHCDLK
  PENILLKQQGRSGIKVIDFGSSCYEHQRVYTYIQSRFYRAPEVILGARYGMPIDMWSLGCILAELLTGYP
  LLPGEDEGDQLACMIELLGMPSQKLLDASKRAKNFVSSKGYPRYCTVTTLSDGSVVLNGGRSRRGKLRGP
  PESREWGNALKGCDDPLFLDFLKQCLEWDPAVRMTPGQALRHPWLRRRLPKPPTGEKTSVKRITESTGAI
  TSISKLPPPSSSASKLRTNLAQMTDANGNIQQRTVLPKLVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006482
• ORF Size: 1803 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006482.3
• RefSeq Size: 6159 bp
• RefSeq ORF: 1806 bp
• Locus ID: 8445
• UniProt ID: Q92630
• Cytogenetics: 12q15
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 67.1 kDa
• Gene Summary: DYRK2 belongs to a family of protein kinases whose members are presumed to be involved in cellular growth and/or development. The family is defined by structural similarity of their kinase domains and their capability to autophosphorylate on tyrosine residues. DYRK2 has demonstrated tyrosine autophosphorylation and catalyzed phosphorylation of histones H3 and H2B in vitro. Two isoforms of DYRK2 have been isolated. The predominant isoform, isoform 1, lacks a 5' terminal insert. [provided by RefSeq, Jul 2008]