DYRK2 (NM_006482) Human Untagged Clone

CAT#: SC323580

DYRK2 (untagged)-Kinase deficient mutant (K251M) of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DYRK2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323580 sequence for NM_006482 edited (data generated by NextGen Sequencing)
  ATGTTAACCAGGAAACCTTCGGCCGCCGCTCCCGCCGCCTACCCGACCGGCCGAGGTGGG
  GACAGCGCCGTTCGTCAGCTTCAGGCTTCCCCGGGGCTCGGTGCAGGGGCCACCCGGAGC
  GGAGTGGGGACTGGCCCGCCCTCCCCCATCGCCCTGCCGCCTCTCCGGGCCAGCAACGCT
  GCCGCCGCAGCCCACACGATTGGCGGCAGTAAGCACACAATGAATGATCACCTGCATGTC
  GGCAGCCACGCTCACGGACAGATCCAGGTTCAACAGTTGTTTGAGGATAACAGTAACAAG
  CGGACAGTGCTCACGACACAACCAAATGGGCTTACAACAGTGGGCAAAACGGGCTTGCCA
  GTGGTGCCAGAGCGGCAGCTGGACAGCATTCATAGACGGCAGGGGAGCTCCACCTCTCTA
  AAGTCCATGGAAGGCATGGGGAAGGTGAAAGCCACCCCCATGACACCTGAACAAGCAATG
  AAGCAATACATGCAAAAACTCACAGCCTTCGAACACCATGAGATTTTCAGCTACCCTGAA
  ATATATTTCTTGGGTCTAAATGCTAAGAAGCGCCAGGGCATGACAGGTGGGCCCAACAAT
  GGTGGCTATGATGATGACCAGGGATCATATGTGCAGGTGCCCCACGATCACGTGGCTTAC
  AGGTATGAGGTCCTCAAGGTCATTGGGAAGGGGAGCTTTGGGCAGGTGGTCAAGGCCTAC
  GATCACAAAGTCCACCAGCACGTGGCCCTAATGATGGTGCGGAATGAGAAGCGCTTCCAC
  CGGCAAGCAGCGGAGGAGATCCGAATCCTGGAACACCTGCGGAAGCAGGACAAGGATAAC
  ACAATGAATGTCATCCATATGCTGGAGAATTTCACCTTCCGCAACCACATCTGCATGACG
  TTTGAGCTGCTGAGCATGAACCTCTATGAGCTCATCAAGAAGAATAAATTCCAGGGCTTC
  AGTCTGCCTTTGGTTCGCAAGTTTGCCCACTCGATTCTGCAGTGCTTGGATGCTTTGCAC
  AAAAACAGAATAATTCACTGTGACCTTAAGCCCGAGAACATTTTGTTAAAGCAGCAGGGT
  AGAAGCGGTATTAAAGTAATTGATTTTGGCTCCAGTTGTTACGAGCATCAGCGTGTCTAC
  ACGTACATCCAGTCGCGTTTTTACCGGGCTCCAGAAGTGATCCTTGGGGCCAGGTATGGC
  ATGCCCATTGATATGTGGAGCCTGGGCTGCATTTTAGCAGAGCTCCTGACGGGTTACCCC
  CTCTTGCCTGGGGAAGATGAAGGGGACCAGCTGGCCTGTATGATTGAACTGTTGGGCATG
  CCCTCACAGAAACTGCTGGATGCATCCAAACGAGCCAAAAATTTTGTGAGCTCCAAGGGT
  TATCCCCGTTACTGCACTGTCACGACTCTCTCAGATGGCTCTGTGGTCCTAAACGGAGGC
  CGTTCCCGGAGGGGGAAACTGAGGGGCCCACCGGAGAGCAGAGAGTGGGGGAACGCGCTG
  AAGGGGTGTGATGATCCCCTTTTCCTTGACTTCTTAAAACAGTGTTTAGAGTGGGATCCT
  GCAGTGCGCATGACCCCAGGCCAGGCTTTGCGGCACCCCTGGCTGAGGAGGCGGTTGCCA
  AAGCCTCCCACCGGGGAGAAAACGTCAGTGAAAAGGATAACTGAGAGCACCGGTGCTATC
  ACATCTATATCCAAGTTACCTCCACCTTCTAGCTCAGCTTCCAAACTGAGGACTAATTTG
  GCGCAGATGACAGATGCCAATGGGAATATTCAGCAGAGGACAGTGTTGCCAAAACTTGTT
  AGCTGA
  
  Clone variation with respect to NM_006482.2
  752 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_006482 unedited
  CCGCCCGTTACAGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTTCTATATAAGCAGAGCTCGTTTAGTG
  AACCGTCAGAATATTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGAGCGGGGG
  GCTCGCGGCGGCGGGCCCCGGCCGAGGGGATGCAGTGGACTGTGTGTGTCTGGCTGTAGCAGACGCGAGG
  CGGCGACGAGGCGCCGGGGACCCGCGCGAGGGGCGGCCGGGAGGCGGCGGCGGCGGCCGCCAGAGTAGCA
  GCAGACCGGCGGCGGCGACGGCAGCCCTGAATGCATTTTCCTCTCCACGCCCCGTGGGGGGGGGGGGCGG
  AGGCCGCGCTACGCCGCTAACCGACCGCCGGGTGTGACACCGCGTTCGGGGGGTGCGGTTCCCGGGACTC
  GTGATGAACCACAAAAAATAAATG
  
  Kinase domain sequence

  >SC323580 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TAGTATMMGTGGCTTAAGGTATGAGGTCCTCAGGTCATTGGGAAGGGGAGCTTTGGGCAGGTGGTCAAGG
  CCTACGATCACAAAGTCCACCAGCACGTGGCCCTAATGATGGTGCGGAATGAGAAGCGCTTCCACCGGCA
  AGCAGCGGAGGAGATCCGAATCCTGGAACACCTGCGGAAGCAGGACAAGGATAACACAATGAATGTCATC
  CATATGCTGGAGAATTTCACCTTCCGCAACCACATCTGCATGACG
  
• Restriction Sites: Please inquire
• ACCN: NM_006482
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006482.1, NP_006473.1
• RefSeq Size: 3615 bp
• RefSeq ORF: 1806 bp
• Locus ID: 8445
• UniProt ID: Q92630
• Cytogenetics: 12q15
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: DYRK2 belongs to a family of protein kinases whose members are presumed to be involved in cellular growth and/or development. The family is defined by structural similarity of their kinase domains and their capability to autophosphorylate on tyrosine residues. DYRK2 has demonstrated tyrosine autophosphorylation and catalyzed phosphorylation of histones H3 and H2B in vitro. Two isoforms of DYRK2 have been isolated. The predominant isoform, isoform 1, lacks a 5' terminal insert. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) includes the 149 bp insert in the 5' end of the transcript and thus results in a longer amino-terminal protein.