RAB14 (NM_016322) Human Tagged ORF Clone
RAB14 (Myc-DDK-tagged)-Human RAB14, member RAS oncogene family (RAB14)
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC202832 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC202832 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||645 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||4379 bp|
|RefSeq ORF||648 bp|
|Domains||ras, RAN, RAS, RHO, RAB|
|Protein Families||Druggable Genome|
|Gene Summary||RAB14 belongs to the large RAB family of low molecular mass GTPases that are involved in intracellular membrane trafficking. These proteins act as molecular switches that flip between an inactive GDP-bound state and an active GTP-bound state in which they recruit downstream effector proteins onto membranes (Junutula et al., 2004 [PubMed 15004230]).[supplied by OMIM, Mar 2009]|
|cDNA Clone Resources|
|RC202832L3||Lenti ORF clone of Human RAB14, member RAS oncogene family (RAB14), Myc-DDK-tagged||
|RC202832L4||Lenti ORF clone of Human RAB14, member RAS oncogene family (RAB14), mGFP tagged||
|RG202832||RAB14 (GFP-tagged) - Human RAB14, member RAS oncogene family (RAB14)||
|SC321525||RAB14 (untagged)-Human RAB14, member RAS oncogene family (RAB14)||