RAB14 (NM_016322) Human Tagged ORF Clone

CAT#: RC202832

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RAB14 (Myc-DDK-tagged)-Human RAB14, member RAS oncogene family (RAB14)



  "NM_016322" in other vectors (4)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 429.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol RAB14
Synonyms FBP; RAB-14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202832 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAACTGCACCATACAACTACTCTTACATCTTTAAATATATTATTATTGGGGACATGGGAGTAGGAA
AATCTTGCTTGCTTCATCAATTTACAGAAAAAAAATTTATGGCTGATTGTCCTCACACAATTGGTGTTGA
ATTTGGTACAAGAATAATCGAAGTTAGTGGCCAAAAAATAAAACTGCAGATTTGGGATACGGCAGGACAG
GAGCGATTTAGGGCTGTTACACGGAGCTACTACAGAGGAGCTGCGGGAGCTCTTATGGTCTATGATATCA
CTAGAAGAAGTACATATAACCACTTAAGCAGCTGGTTGACAGATGCAAGGAATCTCACCAATCCAAATAC
TGTAATAATTCTCATAGGAAATAAAGCAGATTTGGAGGCACAGAGAGATGTTACATATGAAGAAGCCAAA
CAGTTTGCTGAAGAAAATGGCTTATTGTTCCTCGAAGCGAGTGCAAAAACGGGAGAGAATGTAGAAGATG
CCTTCCTTGAGGCTGCCAAGAAAATCTATCAGAACATTCAGGATGGAAGCTTGGATCTGAATGCTGCTGA
GTCTGGTGTACAACACAAACCTTCAGCCCCGCAGGGAGGCCGGCTAACCAGTGAACCCCAACCCCAGAGA
GAAGGCTGTGGCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202832 protein sequence
Red=Cloning site Green=Tags(s)

MATAPYNYSYIFKYIIIGDMGVGKSCLLHQFTEKKFMADCPHTIGVEFGTRIIEVSGQKIKLQIWDTAGQ
ERFRAVTRSYYRGAAGALMVYDITRRSTYNHLSSWLTDARNLTNPNTVIILIGNKADLEAQRDVTYEEAK
QFAEENGLLFLEASAKTGENVEDAFLEAAKKIYQNIQDGSLDLNAAESGVQHKPSAPQGGRLTSEPQPQR
EGCGC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016322
ORF Size 645 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016322.4
RefSeq Size 4379 bp
RefSeq ORF 648 bp
Locus ID 51552
UniProt ID P61106
Cytogenetics 9q33.2
Domains ras, RAN, RAS, RHO, RAB
Protein Families Druggable Genome
MW 23.9 kDa
Gene Summary RAB14 belongs to the large RAB family of low molecular mass GTPases that are involved in intracellular membrane trafficking. These proteins act as molecular switches that flip between an inactive GDP-bound state and an active GTP-bound state in which they recruit downstream effector proteins onto membranes (Junutula et al., 2004 [PubMed 15004230]).[supplied by OMIM, Mar 2009]

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