PRIM1 (NM_000946) Human Tagged ORF Clone

CAT#: RC202710

PRIM1 (Myc-DDK-tagged)-Human primase, DNA, polypeptide 1 (49kDa) (PRIM1)

Product Images

Circular map for RC202710

• 

  Western blot validation of overexpression lysate (Cat# LY424441) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202710 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRIM1
• Synonyms: p49
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202710 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACGTTTGACCCCACCGAGCTGCCCGAGCTGCTTAAACTTTATTACCGGAGGCTCTTTCCCTACT
  CTCAGTACTATCGCTGGCTCAACTACGGTGGAGTGATAAAGAATTACTTTCAACACCGTGAATTTTCATT
  CACATTGAAAGATGATATTTACATTCGCTACCAATCCTTCAACAACCAGAGTGATCTGGAAAAGGAGATG
  CAGAAAATGAATCCATACAAGATTGATATAGGCGCAGTATATTCTCACAGACCCAATCAACACAATACAG
  TGAAGCTGGGAGCTTTCCAGGCTCAGGAAAAAGAACTGGTATTTGACATTGACATGACAGACTATGACGA
  TGTGAGGAGATGTTGTAGTTCTGCAGACATATGTCCTAAGTGCTGGACCCTCATGACAATGGCCATACGC
  ATCATTGACAGAGCATTGAAGGAGGACTTTGGATTTAAGCATCGTCTCTGGGTATATTCTGGAAGGAGAG
  GTGTTCATTGTTGGGTCTGTGATGAATCAGTTAGAAAACTGTCTTCTGCAGTACGTTCTGGGATAGTTGA
  GTATTTGAGCCTTGTAAAGGGTGGTCAAGACGTTAAAAAGAAAGTTCACCTAAGTGAAAAAATTCACCCT
  TTTATCAGAAAATCTATAAACATAATAAAAAAATACTTTGAAGAATATGCCTTGGTTAATCAAGATATTC
  TCGAAAATAAAGAAAGCTGGGATAAGATTTTAGCCCTTGTTCCTGAAACAATTCATGATGAACTTCAACA
  AAGCTTCCAAAAGTCTCACAATTCACTTCAGCGTTGGGAGCACTTGAAGAAAGTAGCCAGCAGATATCAG
  AATAACATCAAAAATGACAAATATGGACCCTGGCTGGAGTGGGAGATTATGCTCCAGTACTGTTTTCCAC
  GGCTGGATATCAATGTCAGCAAAGGAATCAATCATCTACTGAAGAGCCCTTTTAGTGTTCATCCTAAAAC
  AGGTCGCATATCTGTGCCTATTGATTTGCAGAAAGTGGACCAGTTTGATCCATTTACTGTTCCGACCATA
  AGCTTCATCTGCCGTGAATTGGATGCCATTTCCACTAATGAAGAGGAAAAAGAGGAGAATGAAGCTGAAT
  CTGATGTCAAACATAGAACCAGAGATTATAAGAAGACCAGTCTAGCACCTTATGTGAAAGTTTTTGAACA
  TTTTCTTGAAAATCTGGATAAATCCCGAAAAGGAGAACTTCTTAAGAAGAGTGATTTACAAAAAGATTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202710 protein sequence
  Red=Cloning site Green=Tags(s)
  
  METFDPTELPELLKLYYRRLFPYSQYYRWLNYGGVIKNYFQHREFSFTLKDDIYIRYQSFNNQSDLEKEM
  QKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTMAIR
  IIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLSEKIHP
  FIRKSINIIKKYFEEYALVNQDILENKESWDKILALVPETIHDELQQSFQKSHNSLQRWEHLKKVASRYQ
  NNIKNDKYGPWLEWEIMLQYCFPRLDINVSKGINHLLKSPFSVHPKTGRISVPIDLQKVDQFDPFTVPTI
  SFICRELDAISTNEEEKEENEAESDVKHRTRDYKKTSLAPYVKVFEHFLENLDKSRKGELLKKSDLQKDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000946
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000946.3
• RefSeq Size: 1471 bp
• RefSeq ORF: 1263 bp
• Locus ID: 5557
• UniProt ID: P49642
• Cytogenetics: 12q13.3
• Protein Families: Druggable Genome
• Protein Pathways: DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism
• MW: 49.9 kDa
• Gene Summary: The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008]