Transglutaminase 4 (TGM4) (NM_003241) Human Tagged ORF Clone

CAT#: RC202705

TGM4 (Myc-DDK-tagged)-Human transglutaminase 4 (prostate) (TGM4)

Product Images

Circular map for RC202705

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  Western blot validation of overexpression lysate (Cat# LY418817) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202705 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TGM4 protein (Cat# TP302705). The protein was produced from HEK293T cells transfected with TGM4 cDNA clone (Cat# RC202705) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Transglutaminase 4
• Synonyms: hTGP; TGP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202705 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGATGCATCAAAAGAGCTGCAAGTTCTCCACATTGACTTCTTGAATCAGGACAACGCCGTTTCTC
  ACCACACATGGGAGTTCCAAACGAGCAGTCCTGTGTTCCGGCGAGGACAGGTGTTTCACCTGCGGCTGGT
  GCTGAACCAGCCCCTACAATCCTACCACCAACTGAAACTGGAATTCAGCACAGGGCCGAATCCTAGCATC
  GCCAAACACACCCTGGTGGTGCTCGACCCGAGGACGCCCTCAGACCACTACAACTGGCAGGCAACCCTTC
  AAAATGAGTCTGGCAAAGAGGTCACAGTGGCTGTCACCAGTTCCCCCAATGCCATCCTGGGCAAGTACCA
  ACTAAACGTGAAAACTGGAAACCACATCCTTAAGTCTGAAGAAAACATCCTATACCTTCTCTTCAACCCA
  TGGTGTAAAGAGGACATGGTTTTCATGCCTGATGAGGACGAGCGCAAAGAGTACATCCTCAATGACACGG
  GCTGCCATTACGTGGGGGCTGCCAGAAGTATCAAATGCAAACCCTGGAACTTTGGTCAGTTTGAGAAAAA
  TGTCCTGGACTGCTGCATTTCCCTGCTGACTGAGAGCTCCCTCAAGCCCACAGATAGGAGGGACCCCGTG
  CTGGTGTGCAGGGCCATGTGTGCTATGATGAGCTTTGAGAAAGGCCAGGGCGTGCTCATTGGGAATTGGA
  CTGGGGACTACGAAGGTGGCACAGCCCCATACAAGTGGACAGGCAGTGCCCCGATCCTGCAGCAGTACTA
  CAACACGAAGCAGGCTGTGTGCTTTGGCCAGTGCTGGGTGTTTGCTGGGATCCTGACTACAGTGCTGAGA
  GCGTTGGGCATCCCAGCACGCAGTGTGACAGGCTTCGATTCAGCTCACGACACAGAAAGGAACCTCACGG
  TGGACACCTATGTGAATGAGAATGGCGAGAAAATCACCAGTATGACCCACGACTCTGTCTGGAATTTCCA
  TGTGTGGACGGATGCCTGGATGAAGCGACCGGATCTGCCCAAGGGCTACGACGGCTGGCAGGCTGTGGAC
  GCAACGTCGCAGGAGCGAAGCCAGGGTGTCTTCTGTTGTGGGCCATCACCACTGACCGCCATCCGCAAAG
  GTGACATCTTTATTGTCTATGACACCAGATTCGTCTTCTCAGAAGTGAATGGTGACAGGCTCATCTGGTT
  GGTGAAGATGGTGAATGGGCAGGAGGAGTTACACGTAATTTCAATGGAGACCACAAGCATCGGGAAAAAC
  ATCAGCACCAAGGCAGTGGGCCAAGACAGGCGGAGAGATATCACCTATGAGTACAAGTATCCAGAAGGCT
  CCTCTGAGGAGAGGCAGGTCATGGATCATGCCTTCCTCCTTCTCAGTTCTGAGAGGGAGCACAGACGACC
  TGTAAAAGAGAACTTTCTTCACATGTCGGTACAATCAGATGATGTGCTGCTGGGAAACTCTGTTAATTTC
  ACCGTGATTCTTAAAAGGAAGACCGCTGCCCTACAGAATGTCAACATCTTGGGTTCCTTTGAACTACAGT
  TGTACACTGGCAAGAAGATGGCAAAACTGTGTGACCTCAATAAGACCTCGCAGATCCAAGGTCAAGTATC
  AGAAGTGACTCTGACCTTGGACTCCAAGACCTACATCAACAGCCTGGCTATATTAGATGATGAGCCAGTT
  ATCAGAGGTTTCATCATTGCGGAAATTGTGGAGTCTAAGGAAATCATGGCCTCTGAAGTATTCACGTCTT
  TCCAGTACCCTGAGTTCTCTATAGAGTTGCCTAACACAGGCAGAATTGGCCAGCTACTTGTCTGCAATTG
  TATCTTCAAGAATACCCTGGCCATCCCTTTGACTGACGTCAAGTTCTCTTTGGAAAGCCTGGGCATCTCC
  TCACTACAGACCTCTGACCATGGGACGGTGCAGCCTGGTGAGACCATCCAATCCCAAATAAAATGCACCC
  CAATAAAAACTGGACCCAAGAAATTTATCGTCAAGTTAAGTTCCAAACAAGTGAAAGAGATTAATGCTCA
  GAAGATTGTTCTCATCACCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202705 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMDASKELQVLHIDFLNQDNAVSHHTWEFQTSSPVFRRGQVFHLRLVLNQPLQSYHQLKLEFSTGPNPSI
  AKHTLVVLDPRTPSDHYNWQATLQNESGKEVTVAVTSSPNAILGKYQLNVKTGNHILKSEENILYLLFNP
  WCKEDMVFMPDEDERKEYILNDTGCHYVGAARSIKCKPWNFGQFEKNVLDCCISLLTESSLKPTDRRDPV
  LVCRAMCAMMSFEKGQGVLIGNWTGDYEGGTAPYKWTGSAPILQQYYNTKQAVCFGQCWVFAGILTTVLR
  ALGIPARSVTGFDSAHDTERNLTVDTYVNENGEKITSMTHDSVWNFHVWTDAWMKRPDLPKGYDGWQAVD
  ATSQERSQGVFCCGPSPLTAIRKGDIFIVYDTRFVFSEVNGDRLIWLVKMVNGQEELHVISMETTSIGKN
  ISTKAVGQDRRRDITYEYKYPEGSSEERQVMDHAFLLLSSEREHRRPVKENFLHMSVQSDDVLLGNSVNF
  TVILKRKTAALQNVNILGSFELQLYTGKKMAKLCDLNKTSQIQGQVSEVTLTLDSKTYINSLAILDDEPV
  IRGFIIAEIVESKEIMASEVFTSFQYPEFSIELPNTGRIGQLLVCNCIFKNTLAIPLTDVKFSLESLGIS
  SLQTSDHGTVQPGETIQSQIKCTPIKTGPKKFIVKLSSKQVKEINAQKIVLITK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003241
• ORF Size: 2052 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003241.4
• RefSeq Size: 3027 bp
• RefSeq ORF: 2055 bp
• Locus ID: 7047
• UniProt ID: P49221
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome
• MW: 77.1 kDa
• Gene Summary: Associated with the mammalian reproductive process. Catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract.[UniProtKB/Swiss-Prot Function]