ATPAF2 (NM_145691) Human Tagged ORF Clone

CAT#: RC202610

ATPAF2 (Myc-DDK-tagged)-Human ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC202610

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  Western blot validation of overexpression lysate (Cat# LY407896) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202610 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATPAF2
• Synonyms: ATP12; ATP12p; LP3663; MC5DN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202610 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGGAGCTGCCTCCGGCTGCGGGACGGGGGACGCCGTCTCCTGAATCGGCCGGCGGGTGGCCCCA
  GCGCTTCTATGAGTCCGGGGCCAACCATCCCGTCTCCAGCCCGGGCTTACGCCCCGCCGACAGAAAGGAA
  GAGGTTTTATCAGAATGTCAGCATCACACAGGGTGAAGGTGGCTTTGAGATAAACCTGGACCACAGGAAG
  CTGAAAACTCCCCAAGCCAAGCTCTTTACCGTCCCCAGCGAGGCCCTGGCCATTGCAGTGGCTACTGAGT
  GGGATTCCCAGCAGGATACCATCAAGTACTACACCATGCACCTGACCACATTGTGCAACACATCATTGGA
  CAACCCAACCCAGAGAAACAAGGATCAGCTGATCCGGGCAGCCGTGAAGTTTCTGGACACCGACACCATC
  TGCTACAGGGTGGAGGAGCCCGAGACATTAGTGGAACTTCAAAGGAATGAGTGGGATCCAATCATCGAAT
  GGGCTGAGAAAAGATACGGCGTGGAGATCAGCTCCTCCACCAGCATAATGGGACCCAGCATCCCTGCCAA
  AACTCGGGAGGTGCTCGTCAGCCACCTGGCATCTTACAACACATGGGCTTTACAAGGGATTGAGTTTGTA
  GCTGCCCAGCTCAAGTCCATGGTGCTAACCTTGGGCCTGATTGACCTGCGCCTGACAGTGGAGCAGGCCG
  TGCTGCTGTCACGCCTGGAGGAGGAGTACCAGATCCAGAAGTGGGGCAACATTGAGTGGGCCCATGACTA
  TGAGCTGCAGGAGCTGCGGGCCCGCACCGCCGCCGGCACCCTCTTCATCCATCTCTGCTCCGAGAGCACC
  ACAGTCAAGCACAAGCTCCTGAAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202610 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWRSCLRLRDGGRRLLNRPAGGPSASMSPGPTIPSPARAYAPPTERKRFYQNVSITQGEGGFEINLDHRK
  LKTPQAKLFTVPSEALAIAVATEWDSQQDTIKYYTMHLTTLCNTSLDNPTQRNKDQLIRAAVKFLDTDTI
  CYRVEEPETLVELQRNEWDPIIEWAEKRYGVEISSSTSIMGPSIPAKTREVLVSHLASYNTWALQGIEFV
  AAQLKSMVLTLGLIDLRLTVEQAVLLSRLEEEYQIQKWGNIEWAHDYELQELRARTAAGTLFIHLCSEST
  TVKHKLLKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145691
• ORF Size: 867 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145691.4
• RefSeq Size: 1552 bp
• RefSeq ORF: 870 bp
• Locus ID: 91647
• UniProt ID: Q8N5M1
• Cytogenetics: 17p11.2
• MW: 32.8 kDa
• Gene Summary: This gene encodes an assembly factor for the F(1) component of the mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. This gene is located within the Smith-Magenis syndrome region on chromosome 17. An alternatively spliced transcript variant has been described, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]