GTF2H4 (NM_001517) Human Tagged ORF Clone

CAT#: RC202555

GTF2H4 (Myc-DDK-tagged)-Human general transcription factor IIH, polypeptide 4, 52kDa (GTF2H4)

Product Images

Circular map for RC202555

• 

  Western blot validation of overexpression lysate (Cat# LY419887) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202555 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GTF2H4
• Synonyms: P52; TFB2; TFIIH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202555 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCACCCCTTCAAGGGGACTGAACCGAGTACACCTACAATGCAGGAATCTGCAGGAATTCTTAG
  GGGGCCTGAGCCCTGGGGTATTGGACCGATTGTATGGGCACCCTGCCACATGTCTGGCTGTCTTCAGGGA
  GCTCCCATCCTTGGCTAAGAACTGGGTGATGCGGATGCTCTTTCTGGAGCAGCCTTTGCCACAGGCTGCT
  GTAGCTCTGTGGGTAAAGAAGGAATTCAGCAAGGCTCAGGAGGAAAGTACAGGGCTGCTGAGCGGCCTCC
  GGATCTGGCACACACAGCTGCTCCCAGGCGGGCTCCAGGGCCTCATCCTCAACCCCATTTTCCGCCAGAA
  CCTCCGCATTGCCCTTCTGGGTGGGGGGAAGGCCTGGTCTGATGACACAAGTCAGCTGGGACCAGACAAG
  CATGCCCGGGACGTTCCCTCCCTTGACAAGTACGCCGAGGAGCGATGGGAGGTGGTCTTGCACTTCATGG
  TGGGCTCCCCCAGTGCAGCTGTCAGCCAGGACTTGGCTCAGCTCCTCAGCCAGGCTGGGCTCATGAAGAG
  TACTGAACCTGGAGAGCCGCCCTGCATTACTTCCGCTGGCTTCCAGTTCCTGTTGCTGGACACCCCGGCT
  CAGCTCTGGTACTTTATGTTGCAGTATTTGCAGACAGCCCAGAGCCGGGGCATGGACCTGGTAGAGATTC
  TCTCCTTCCTCTTCCAGCTCAGCTTCTCTACTCTGGGCAAGGATTACTCTGTGGAAGGTATGAGTGATTC
  TCTGTTGAACTTCCTGCAACATCTGCGTGAGTTTGGGCTTGTTTTCCAGAGGAAGAGGAAATCTCGGCGT
  TACTACCCCACACGCCTGGCCATCAATCTCTCATCAGGTGTCTCTGGAGCTGGGGGCACTGTGCATCAGC
  CAGGTTTCATTGTCGTGGAAACCAATTACCGACTGTATGCCTACACGGAGTCGGAGCTGCAGATTGCCCT
  CATTGCCCTCTTCTCTGAGATGCTCTATCGGTTCCCCAACATGGTGGTGGCGCAGGTGACCCGGGAGAGT
  GTGCAGCAGGCAATCGCCAGTGGCATCACAGCCCAGCAGATAATCCATTTCCTAAGGACAAGAGCCCACC
  CAGTGATGCTCAAACAGACACCTGTGCTGCCCCCCACCATCACCGACCAGATCCGGCTCTGGGAGCTGGA
  AAGGGACAGACTCCGGTTCACTGAGGGTGTCCTGTATAACCAGTTCCTGTCGCAAGTGGACTTTGAGCTG
  CTGCTGGCCCACGCGCGGGAGCTGGGCGTGCTCGTGTTCGAGAACTCGGCCAAGCGGCTCATGGTGGTGA
  CCCCGGCCGGGCACAGCGACGTCAAGCGCTTTTGGAAGCGGCAGAAACATAGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202555 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESTPSRGLNRVHLQCRNLQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAA
  VALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQGLILNPIFRQNLRIALLGGGKAWSDDTSQLGPDK
  HARDVPSLDKYAEERWEVVLHFMVGSPSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPA
  QLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRR
  YYPTRLAINLSSGVSGAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRES
  VQQAIASGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFEL
  LLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001517
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001517.5
• RefSeq Size: 1736 bp
• RefSeq ORF: 1389 bp
• Locus ID: 2968
• UniProt ID: Q92759
• Cytogenetics: 6p21.33
• Domains: Tfb2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Basal transcription factors, Nucleotide excision repair
• MW: 52.2 kDa
• Gene Summary: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.[UniProtKB/Swiss-Prot Function]