PPPDE1 (DESI2) (NM_016076) Human Tagged ORF Clone
CAT#: RC202524
DESI2 (Myc-DDK-tagged)-Human PPPDE peptidase domain containing 1 (PPPDE1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_016076" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PPPDE1 |
Synonyms | C1orf121; CGI-146; DESI; DeSI-2; DESI1; FAM152A; PNAS-4; PPPDE1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202524 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCTAACCAGTTAGTGGTGCTCAACGTGTACGACATGTATTGGATGAACGAATATACCTCATCCA TTGGAATTGGAGTTTTTCATTCAGGAATTGAAGTCTATGGCAGAGAATTTGCTTATGGTGGCCATCCTTA CCCCTTTTCTGGAATATTTGAAATTTCCCCAGGAAATGCTTCTGAACTAGGAGAAACATTTAAATTTAAA GAAGCTGTTGTTTTAGGGAGCACGGACTTCCTAGAAGATGATATAGAAAAAATTGTAGAAGAACTGGGAA AAGAATACAAAGGCAATGCTTATCATTTAATGCATAAAAACTGCAATCATTTTTCTTCAGCTTTATCAGA GATTCTTTGTGGGAAAGAGATTCCTCGCTGGATCAATCGACTTGCCTACTTCAGCTCCTGTATACCCTTT CTACAGAGTTGCCTCCCGAAGGAGTGGCTCACGCCCGCAGCCCTGCAGTCTAGTGTCAGCCAAGAACTCC AGGATGAACTGGAGGAAGCAGAGGATGCTGCCGCATCCGCTTCCGTGGCAAGCACTGCAGCAGGCTCCAG ACCCGGGCGCCACACTAAACTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202524 protein sequence
Red=Cloning site Green=Tags(s) MGANQLVVLNVYDMYWMNEYTSSIGIGVFHSGIEVYGREFAYGGHPYPFSGIFEISPGNASELGETFKFK EAVVLGSTDFLEDDIEKIVEELGKEYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLAYFSSCIPF LQSCLPKEWLTPAALQSSVSQELQDELEEAEDAAASASVASTAAGSRPGRHTKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_016076 |
ORF Size | 582 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016076.5 |
RefSeq Size | 4109 bp |
RefSeq ORF | 585 bp |
Locus ID | 51029 |
UniProt ID | Q9BSY9 |
Cytogenetics | 1q44 |
MW | 21.4 kDa |
Gene Summary | Has deubiquitinating activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Deubiquitinates 'Lys-48'-linked polyubiquitination of RPS7 leading to its stabilization (PubMed:28483520).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202524L3 | Lenti ORF clone of Human PPPDE peptidase domain containing 1 (PPPDE1), Myc-DDK-tagged |
USD 600.00 |
|
RC202524L4 | Lenti ORF clone of Human PPPDE peptidase domain containing 1 (PPPDE1), mGFP tagged |
USD 600.00 |
|
RG202524 | DESI2 (tGFP-tagged) - Human PPPDE peptidase domain containing 1 (PPPDE1) |
USD 500.00 |
|
SC319079 | DESI2 (untagged)-Human PPPDE peptidase domain containing 1 (PPPDE1) |
USD 300.00 |
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