MAGEA10 (NM_001011543) Human Tagged ORF Clone

CAT#: RC202501

MAGEA10 (Myc-DDK-tagged)-Human melanoma antigen family A, 10 (MAGEA10), transcript variant 1

Product Images

Circular map for RC202501

• 

  Western blot validation of overexpression lysate (Cat# LY412120) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223638 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAGEA10
• Synonyms: CT1.10; MAGE10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202501 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCGAGCTCCAAAGCGTCAGCGCTGCATGCCTGAAGAAGATCTTCAATCCCAAAGTGAGACACAGG
  GCCTCGAGGGTGCACAGGCTCCCCTGGCTGTGGAGGAGGATGCTTCATCATCCACTTCCACCAGCTCCTC
  TTTTCCATCCTCTTTTCCCTCCTCCTCCTCTTCCTCCTCCTCCTCCTGCTATCCTCTAATACCAAGCACC
  CCAGAGGAGGTTTCTGCTGATGATGAGACACCAAATCCTCCCCAGAGTGCTCAGATAGCCTGCTCCTCCC
  CCTCGGTCGTTGCTTCCCTTCCATTAGATCAATCTGATGAGGGCTCCAGCAGCCAAAAGGAGGAGAGTCC
  AAGCACCCTACAGGTCCTGCCAGACAGTGAGTCTTTACCCAGAAGTGAGATAGATGAAAAGGTGACTGAT
  TTGGTGCAGTTTCTGCTCTTCAAGTATCAAATGAAGGAGCCGATCACAAAGGCAGAAATACTGGAGAGTG
  TCATAAAAAATTATGAAGACCACTTCCCTTTGTTGTTTAGTGAAGCCTCCGAGTGCATGCTGCTGGTCTT
  TGGCATTGATGTAAAGGAAGTGGATCCCACTGGCCACTCCTTTGTCCTTGTCACCTCCCTGGGCCTCACC
  TATGATGGGATGCTGAGTGATGTCCAGAGCATGCCCAAGACTGGCATTCTCATACTTATCCTAAGCATAA
  TCTTCATAGAGGGCTACTGCACCCCTGAGGAGGTCATCTGGGAAGCACTGAATATGATGGGGCTGTATGA
  TGGGATGGAGCACCTCATTTATGGGGAGCCCAGGAAGCTGCTCACCCAAGATTGGGTGCAGGAAAACTAC
  CTGGAGTACCGGCAGGTGCCTGGCAGTGATCCTGCACGGTATGAGTTTCTGTGGGGTCCAAGGGCTCATG
  CTGAAATTAGGAAGATGAGTCTCCTGAAATTTTTGGCCAAGGTAAATGGGAGTGATCCAAGATCCTTCCC
  ACTGTGGTATGAGGAGGCTTTGAAAGATGAGGAAGAGAGAGCCCAGGACAGAATTGCCACCACAGATGAT
  ACTACTGCCATGGCCAGTGCAAGTTCTAGCGCTACAGGTAGCTTCTCCTACCCTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202501 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPRAPKRQRCMPEEDLQSQSETQGLEGAQAPLAVEEDASSSTSTSSSFPSSFPSSSSSSSSSCYPLIPST
  PEEVSADDETPNPPQSAQIACSSPSVVASLPLDQSDEGSSSQKEESPSTLQVLPDSESLPRSEIDEKVTD
  LVQFLLFKYQMKEPITKAEILESVIKNYEDHFPLLFSEASECMLLVFGIDVKEVDPTGHSFVLVTSLGLT
  YDGMLSDVQSMPKTGILILILSIIFIEGYCTPEEVIWEALNMMGLYDGMEHLIYGEPRKLLTQDWVQENY
  LEYRQVPGSDPARYEFLWGPRAHAEIRKMSLLKFLAKVNGSDPRSFPLWYEEALKDEEERAQDRIATTDD
  TTAMASASSSATGSFSYPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011543
• ORF Size: 1107 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011543.1, NP_001011543.1
• RefSeq Size: 2759 bp
• RefSeq ORF: 1110 bp
• Locus ID: 4109
• UniProt ID: P43363
• Cytogenetics: Xq28
• MW: 40.8 kDa
• Gene Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream melanoma antigen family A, 5 (MAGEA5) gene.[provided by RefSeq, Oct 2011]