PLK2 (NM_006622) Human Tagged ORF Clone

CAT#: RC202499

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PLK2 (Myc-DDK-tagged)-Human polo-like kinase 2 (PLK2)



  "NM_006622" in other vectors (7)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 941.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PLK2
Synonyms hPlk2; hSNK; SNK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202499 representing NM_006622
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTTTTGCGGACTATCACCTACCAGCCAGCCGCCAGCACCAAAATGTGCGAGCAGGCGCTGGGCA
AGGGTTGCGGAGCAGACTCGAAGAAGAAGCGGCCGCCGCAGCCCCCCGAGGAATCGCAGCCACCTCAGTC
CCAGGCGCAAGTGCCCCCGGCGGCCCCTCACCACCATCACCACCATTCGCACTCGGGGCCGGAGATCTCG
CGGATTATCGTCGACCCCACGACTGGGAAGCGCTACTGCCGGGGCAAAGTGCTGGGAAAGGGTGGCTTTG
CAAAATGTTACGAGATGACAGATTTGACAAATAACAAAGTCTACGCCGCAAAAATTATTCCTCACAGCAG
AGTAGCTAAACCTCATCAAAGGGAAAAGATTGACAAAGAAATAGAGCTTCACAGAATTCTTCATCATAAG
CATGTAGTGCAGTTTTACCACTACTTCGAGGACAAAGAAAACATTTACATTCTCTTGGAATACTGCAGTA
GAAGGTCAATGGCTCATATTTTGAAAGCAAGAAAGGTGTTGACAGAGCCAGAAGTTCGATACTACCTCAG
GCAGATTGTGTCTGGACTGAAATACCTTCATGAACAAGAAATCTTGCACAGAGATCTCAAACTAGGGAAC
TTTTTTATTAATGAAGCCATGGAACTAAAAGTTGGGGACTTCGGTCTGGCAGCCAGGCTAGAACCCTTGG
AACACAGAAGGAGAACGATATGTGGTACCCCAAATTATCTCTCTCCTGAAGTCCTCAACAAACAAGGACA
TGGCTGTGAATCAGACATTTGGGCCCTGGGCTGTGTAATGTATACAATGTTACTAGGGAGGCCCCCATTT
GAAACTACAAATCTCAAAGAAACTTATAGGTGCATAAGGGAAGCAAGGTATACAATGCCGTCCTCATTGC
TGGCTCCTGCCAAGCACTTAATTGCTAGTATGTTGTCCAAAAACCCAGAGGATCGTCCCAGTTTGGATGA
CATCATTCGACATGACTTTTTTTTGCAGGGCTTCACTCCGGACAGACTGTCTTCTAGCTGTTGTCATACA
GTTCCAGATTTCCACTTATCAAGCCCAGCTAAGAATTTCTTTAAGAAAGCAGCTGCTGCTCTTTTTGGTG
GCAAAAAAGACAAAGCAAGATATATTGACACACATAATAGAGTGTCTAAAGAAGATGAAGACATCTACAA
GCTTAGGCATGATTTGAAAAAGACTTCAATAACTCAGCAACCCAGCAAACACAGGACAGATGAGGAGCTC
CAGCCACCTACCACCACAGTTGCCAGGTCTGGAACACCCGCAGTAGAAAACAAGCAGCAGATTGGGGATG
CTATTCGGATGATAGTCAGAGGGACTCTTGGCAGCTGTAGCAGCAGCAGTGAATGCCTTGAAGACAGTAC
CATGGGAAGTGTTGCAGACACAGTGGCAAGGGTTCTTCGGGGATGTCTGGAAAACATGCCGGAAGCTGAT
TGCATTCCCAAAGAGCAGCTGAGCACATCATTTCAGTGGGTCACCAAATGGGTTGATTACTCTAACAAAT
ATGGCTTTGGGTACCAGCTCTCAGACCACACCGTCGGTGTCCTTTTCAACAATGGTGCTCACATGAGCCT
CCTTCCAGACAAAAAAACAGTTCACTATTACGCAGAGCTTGGCCAATGCTCAGTTTTCCCAGCAACAGAT
GCTCCTGAGCAATTTATTAGTCAAGTGACGGTGCTGAAATACTTTTCTCATTACATGGAGGAGAACCTCA
TGGATGGTGGAGATCTGCCTAGTGTTACTGATATTCGAAGACCTCGGCTCTACCTCCTTCAGTGGCTAAA
ATCTGATAAGGCCCTAATGATGCTCTTTAATGATGGCACCTTTCAGGTGAATTTCTACCATGATCATACA
AAAATCATCATCTGTAGCCAAAATGAAGAATACCTTCTCACCTACATCAATGAGGATAGGATATCTACAA
CTTTCAGGCTGACAACTCTGCTGATGTCTGGCTGTTCATCAGAATTAAAAAATCGAATGGAATATGCCCT
GAACATGCTCTTACAAAGATGTAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202499 representing NM_006622
Red=Cloning site Green=Tags(s)

MELLRTITYQPAASTKMCEQALGKGCGADSKKKRPPQPPEESQPPQSQAQVPPAAPHHHHHHSHSGPEIS
RIIVDPTTGKRYCRGKVLGKGGFAKCYEMTDLTNNKVYAAKIIPHSRVAKPHQREKIDKEIELHRILHHK
HVVQFYHYFEDKENIYILLEYCSRRSMAHILKARKVLTEPEVRYYLRQIVSGLKYLHEQEILHRDLKLGN
FFINEAMELKVGDFGLAARLEPLEHRRRTICGTPNYLSPEVLNKQGHGCESDIWALGCVMYTMLLGRPPF
ETTNLKETYRCIREARYTMPSSLLAPAKHLIASMLSKNPEDRPSLDDIIRHDFFLQGFTPDRLSSSCCHT
VPDFHLSSPAKNFFKKAAAALFGGKKDKARYIDTHNRVSKEDEDIYKLRHDLKKTSITQQPSKHRTDEEL
QPPTTTVARSGTPAVENKQQIGDAIRMIVRGTLGSCSSSSECLEDSTMGSVADTVARVLRGCLENMPEAD
CIPKEQLSTSFQWVTKWVDYSNKYGFGYQLSDHTVGVLFNNGAHMSLLPDKKTVHYYAELGQCSVFPATD
APEQFISQVTVLKYFSHYMEENLMDGGDLPSVTDIRRPRLYLLQWLKSDKALMMLFNDGTFQVNFYHDHT
KIIICSQNEEYLLTYINEDRISTTFRLTTLLMSGCSSELKNRMEYALNMLLQRCN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006622
ORF Size 2055 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006622.4
RefSeq Size 2972 bp
RefSeq ORF 2058 bp
Locus ID 10769
UniProt ID Q9NYY3
Cytogenetics 5q11.2
Domains pkinase, POLO_box, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
MW 78.1 kDa
Gene Summary The protein encoded by this gene is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. This gene is most abundantly expressed in testis, spleen and fetal tissues, and its expression is inducible by serum, suggesting that it may also play an important role in cells undergoing rapid cell division. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

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