SUZ12 (NM_015355) Human Tagged ORF Clone

CAT#: RC202362

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SUZ12 (Myc-DDK-tagged)-Human suppressor of zeste 12 homolog (Drosophila) (SUZ12)



  "NM_015355" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 691.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SUZ12
Synonyms CHET9; IMMAS; JJAZ1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202362 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCTCAGAAGCACGGCGGTGGGGGAGGGGGCGGCTCGGGGCCCAGCGCGGGGTCCGGGGGAGGCG
GCTTCGGGGGTTCGGCGGCGGTGGCGGCGGCGACGGCTTCGGGCGGCAAATCCGGCGGCGGGAGCTGTGG
AGGGGGTGGCAGTTACTCGGCCTCCTCCTCCTCCTCCGCGGCGGCAGCGGCGGGGGCTGCGGTGTTACCG
GTGAAGAAGCCGAAAATGGAGCACGTCCAGGCTGACCACGAGCTTTTCCTCCAGGCCTTTGAGAAGCCAA
CACAGATCTATAGATTTCTTCGAACTCGGAATCTCATAGCACCAATATTTTTGCACAGAACTCTTACTTA
CATGTCTCATCGAAACTCCAGAACAAACATCAAAAGGAAAACATTTAAAGTTGATGATATGTTATCAAAA
GTAGAGAAAATGAAAGGAGAGCAAGAATCTCATAGCTTGTCAGCTCATTTGCAGCTTACGTTTACTGGTT
TCTTCCACAAAAATGATAAGCCATCACCAAACTCAGAAAATGAACAAAATTCTGTTACCCTGGAAGTCCT
GCTTGTGAAAGTTTGCCACAAAAAAAGAAAGGATGTAAGTTGTCCAATAAGGCAAGTTCCCACAGGTAAA
AAGCAGGTGCCTTTGAATCCTGACCTCAATCAAACAAAACCCGGAAATTTCCCGTCCCTTGCAGTTTCCA
GTAATGAATTTGAACCTAGTAACAGCCATATGGTGAAGTCTTACTCGTTGCTATTTAGAGTGACTCGTCC
AGGAAGAAGAGAGTTTAATGGAATGATTAATGGAGAAACCAATGAAAATATTGATGTCAATGAAGAGCTT
CCAGCCAGAAGAAAACGAAATCGTGAGGATGGGGAAAAGACATTTGTTGCACAAATGACAGTATTTGATA
AAAACAGGCGCTTACAGCTTTTAGATGGGGAATATGAAGTAGCCATGCAGGAAATGGAAGAATGTCCAAT
AAGCAAGAAAAGAGCAACATGGGAGACTATTCTTGATGGGAAGAGGCTGCCTCCATTCGAAACATTTTCT
CAGGGACCTACGTTGCAGTTCACTCTTCGTTGGACAGGAGAGACCAATGATAAATCTACGGCTCCTATTG
CCAAACCTCTTGCCACTAGAAATTCAGAGAGTCTCCATCAGGAAAACAAGCCTGGTTCAGTTAAACCTAC
TCAAACTATTGCTGTTAAAGAATCATTGACTACAGATCTACAAACAAGAAAAGAAAAGGATACTCCAAAT
GAAAACCGACAAAAATTAAGAATATTTTATCAGTTTCTCTATAACAACAATACAAGGCAACAAACTGAAG
CAAGAGATGACCTGCATTGCCCTTGGTGTACTCTGAACTGCCGCAAACTTTATAGTTTACTCAAGCATCT
TAAACTCTGCCATAGCAGATTTATCTTCAACTATGTTTATCATCCAAAAGGTGCTAGGATAGATGTTTCT
ATCAATGAGTGTTATGATGGCTCCTATGCAGGAAATCCTCAGGATATTCATCGCCAACCTGGATTTGCTT
TTAGTCGCAACGGACCAGTTAAGAGAACACCTATCACACATATTCTTGTGTGCAGGCCAAAACGAACAAA
AGCAAGCATGTCTGAATTTCTTGAATCTGAAGATGGGGAAGTAGAACAGCAAAGAACATATAGTAGTGGC
CACAATCGTCTGTATTTCCATAGTGATACCTGCTTACCTCTCCGTCCACAAGAAATGGAAGTAGATAGTG
AAGATGAAAAGGATCCTGAATGGCTAAGAGAAAAAACCATTACACAAATTGAAGAGTTTTCTGATGTTAA
TGAAGGAGAGAAAGAAGTGATGAAACTCTGGAATCTCCATGTCATGAAGCATGGGTTTATTGCTGACAAT
CAAATGAATCATGCCTGTATGCTGTTTGTAGAAAATTATGGACAGAAAATAATTAAGAAGAATTTATGTC
GAAACTTCATGCTTCATCTAGTCAGCATGCATGACTTTAATCTTATTAGCATAATGTCAATAGATAAAGC
TGTTACCAAGCTCCGTGAAATGCAGCAAAAATTAGAAAAGGGGGAATCTGCTTCCCCTGCAAACGAAGAA
ATAACTGAAGAACAAAATGGGACAGCAAATGGATTTAGTGAAATTAACTCAAAAGAGAAAGCTTTGGAAA
CAGATAGTGTCTCAGGGGTTTCAAAACAGAGCAAAAAACAAAAACTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202362 protein sequence
Red=Cloning site Green=Tags(s)

MAPQKHGGGGGGGSGPSAGSGGGGFGGSAAVAAATASGGKSGGGSCGGGGSYSASSSSSAAAAAGAAVLP
VKKPKMEHVQADHELFLQAFEKPTQIYRFLRTRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFKVDDMLSK
VEKMKGEQESHSLSAHLQLTFTGFFHKNDKPSPNSENEQNSVTLEVLLVKVCHKKRKDVSCPIRQVPTGK
KQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLLFRVTRPGRREFNGMINGETNENIDVNEEL
PARRKRNREDGEKTFVAQMTVFDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFS
QGPTLQFTLRWTGETNDKSTAPIAKPLATRNSESLHQENKPGSVKPTQTIAVKESLTTDLQTRKEKDTPN
ENRQKLRIFYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVS
INECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSG
HNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADN
QMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEE
ITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015355
ORF Size 2217 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015355.4
RefSeq Size 4477 bp
RefSeq ORF 2220 bp
Locus ID 23512
UniProt ID Q15022
Cytogenetics 17q11.2
Protein Families Transcription Factors
MW 83.1 kDa
Gene Summary This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq, Jul 2009]

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