AFG1L (NM_145315) Human Tagged ORF Clone

CAT#: RC202313

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LACE1 (Myc-DDK-tagged)-Human lactation elevated 1 (LACE1)



  "NM_145315" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol AFG1L
Synonyms AFG1; c222389; LACE1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202313 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCTCCTGGTCGCTCTTGGTTACCCTGCGCCCCTTAGCACAGAGCCCGCTGAGAGGGAGATGTG
TTGGGTGCGGGGCCTGGGCCGCCGCTCTCGCTCCTCTGGCCACCGCCCCTGGGAAGCCCTTTTGGAAAGC
CTATACGGTTCAGACATCCGAGAGCATGACCCCAACTGCCACTTCAGAGACTTATTTGAAAGCTTTGGCC
GTTTGCCATGGACCTCTGGACCACTATGATTTTCTGATCAAAGCTCATGAGCTAAAGGATGATGAACATC
AAAGAAGAGTCATACAGTGTTTGCAGAAATTACACGAGGACCTTAAAGGATACAATATAGAGGCAGAAGG
CCTTTTTTCAAAGCTTTTTTCAAGGAGCAAACCTCCAAGGGGCCTGTATGTTTATGGAGATGTTGGTACA
GGAAAAACAATGGTGATGGACATGTTTTATGCTTATGTGGAAATGAAGAGGAAAAAACGGGTTCATTTTC
ATGGTTTCATGCTAGATGTGCACAAAAGAATACATCGCCTTAAACAGAGTTTGCCAAAAAGGAAACCAGG
ATTCATGGCTAAATCATATGACCCAATAGCTCCCATAGCCGAAGAAATCAGCGAAGAAGCATGTCTCCTA
TGTTTTGATGAATTTCAGGTCACTGACATTGCTGATGCCATGATTCTGAAACAGCTTTTTGAAAATCTGT
TCAAAAACGGGGTCGTCGTTGTGGCAACATCCAACAGGCCACCGGAAGATCTCTATAAAAATGGACTCCA
AAGAGCTAACTTTGTACCATTCATAGCAGTCTTGAAGGAATATTGTAATACAGTCCAGCTAGATTCTGGG
ATAGATTACCGGAAAAGGGAACTTCCTGCTGCAGGAAAACTCTACTACCTCACAAGTGAAGCTGATGTGG
AGGCTGTCATGGATAAGTTGTTTGATGAGCTGGCTCAGAAACAAAATGATTTAACTAGACCAAGGATTCT
AAAAGTGCAAGGCAGAGAGCTGCGCCTGAATAAAGCCTGTGGAACCGTTGCCGACTGCACATTTGAAGAG
CTGTGTGAGAGACCACTTGGAGCCAGTGACTATTTGGAACTATCAAAGAATTTTGATACAATATTTTTAC
GAAACATTCCGCAATTTACTCTGGCAAACAGGACTCAAGGTCGAAGATTCATAACTCTCATCGATAACTT
TTATGATCTCAAGGTGCGTATAATTTGCTCTGCGTCGACTCCTATATCAAGCTTATTTTTGCATCAACAT
CATGACAGTGAGTTGGAGCAAAGCAGAATACTGATGGATGATTTGGGGCTGAGCCAGGATTCAGCAGAAG
GACTCTCCATGTTTACCGGAGAAGAGGAAATCTTTGCATTTCAGCGCACAATTTCCCGACTCACGGAAAT
GCAGACTGAACAGTACTGGAATGAAGGAGACAGAACCAAGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202313 protein sequence
Red=Cloning site Green=Tags(s)

MAASWSLLVTLRPLAQSPLRGRCVGCGAWAAALAPLATAPGKPFWKAYTVQTSESMTPTATSETYLKALA
VCHGPLDHYDFLIKAHELKDDEHQRRVIQCLQKLHEDLKGYNIEAEGLFSKLFSRSKPPRGLYVYGDVGT
GKTMVMDMFYAYVEMKRKKRVHFHGFMLDVHKRIHRLKQSLPKRKPGFMAKSYDPIAPIAEEISEEACLL
CFDEFQVTDIADAMILKQLFENLFKNGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCNTVQLDSG
IDYRKRELPAAGKLYYLTSEADVEAVMDKLFDELAQKQNDLTRPRILKVQGRELRLNKACGTVADCTFEE
LCERPLGASDYLELSKNFDTIFLRNIPQFTLANRTQGRRFITLIDNFYDLKVRIICSASTPISSLFLHQH
HDSELEQSRILMDDLGLSQDSAEGLSMFTGEEEIFAFQRTISRLTEMQTEQYWNEGDRTKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_145315
ORF Size 1443 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_145315.5
RefSeq Size 2262 bp
RefSeq ORF 1446 bp
Locus ID 246269
UniProt ID Q8WV93
Cytogenetics 6q21
MW 54.8 kDa
Gene Summary This gene encodes a mitochondrial integral membrane protein that plays a role in mitochondrial protein homeostasis. The protein contains a P-loop motif and a five-domain structure that is conserved in fly, yeast, and bacteria. It functions to mediate the degradation of nuclear-encoded complex IV subunits. Two conserved estrogen receptor binding sites are located within 2.5 kb of this gene. Polymorphisms in this gene have been associated with bipolar disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2016]

Other Versions

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