AFG1L (NM_145315) Human Tagged ORF Clone

CAT#: RC202313

LACE1 (Myc-DDK-tagged)-Human lactation elevated 1 (LACE1)

Product Images

Circular map for RC202313

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  Western blot validation of overexpression lysate (Cat# LY407962) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202313 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AFG1L protein (Cat# TP302313). The protein was produced from HEK293T cells transfected with AFG1L cDNA clone (Cat# RC202313) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AFG1L
• Synonyms: AFG1; c222389; LACE1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202313 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTCCTGGTCGCTCTTGGTTACCCTGCGCCCCTTAGCACAGAGCCCGCTGAGAGGGAGATGTG
  TTGGGTGCGGGGCCTGGGCCGCCGCTCTCGCTCCTCTGGCCACCGCCCCTGGGAAGCCCTTTTGGAAAGC
  CTATACGGTTCAGACATCCGAGAGCATGACCCCAACTGCCACTTCAGAGACTTATTTGAAAGCTTTGGCC
  GTTTGCCATGGACCTCTGGACCACTATGATTTTCTGATCAAAGCTCATGAGCTAAAGGATGATGAACATC
  AAAGAAGAGTCATACAGTGTTTGCAGAAATTACACGAGGACCTTAAAGGATACAATATAGAGGCAGAAGG
  CCTTTTTTCAAAGCTTTTTTCAAGGAGCAAACCTCCAAGGGGCCTGTATGTTTATGGAGATGTTGGTACA
  GGAAAAACAATGGTGATGGACATGTTTTATGCTTATGTGGAAATGAAGAGGAAAAAACGGGTTCATTTTC
  ATGGTTTCATGCTAGATGTGCACAAAAGAATACATCGCCTTAAACAGAGTTTGCCAAAAAGGAAACCAGG
  ATTCATGGCTAAATCATATGACCCAATAGCTCCCATAGCCGAAGAAATCAGCGAAGAAGCATGTCTCCTA
  TGTTTTGATGAATTTCAGGTCACTGACATTGCTGATGCCATGATTCTGAAACAGCTTTTTGAAAATCTGT
  TCAAAAACGGGGTCGTCGTTGTGGCAACATCCAACAGGCCACCGGAAGATCTCTATAAAAATGGACTCCA
  AAGAGCTAACTTTGTACCATTCATAGCAGTCTTGAAGGAATATTGTAATACAGTCCAGCTAGATTCTGGG
  ATAGATTACCGGAAAAGGGAACTTCCTGCTGCAGGAAAACTCTACTACCTCACAAGTGAAGCTGATGTGG
  AGGCTGTCATGGATAAGTTGTTTGATGAGCTGGCTCAGAAACAAAATGATTTAACTAGACCAAGGATTCT
  AAAAGTGCAAGGCAGAGAGCTGCGCCTGAATAAAGCCTGTGGAACCGTTGCCGACTGCACATTTGAAGAG
  CTGTGTGAGAGACCACTTGGAGCCAGTGACTATTTGGAACTATCAAAGAATTTTGATACAATATTTTTAC
  GAAACATTCCGCAATTTACTCTGGCAAACAGGACTCAAGGTCGAAGATTCATAACTCTCATCGATAACTT
  TTATGATCTCAAGGTGCGTATAATTTGCTCTGCGTCGACTCCTATATCAAGCTTATTTTTGCATCAACAT
  CATGACAGTGAGTTGGAGCAAAGCAGAATACTGATGGATGATTTGGGGCTGAGCCAGGATTCAGCAGAAG
  GACTCTCCATGTTTACCGGAGAAGAGGAAATCTTTGCATTTCAGCGCACAATTTCCCGACTCACGGAAAT
  GCAGACTGAACAGTACTGGAATGAAGGAGACAGAACCAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202313 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAASWSLLVTLRPLAQSPLRGRCVGCGAWAAALAPLATAPGKPFWKAYTVQTSESMTPTATSETYLKALA
  VCHGPLDHYDFLIKAHELKDDEHQRRVIQCLQKLHEDLKGYNIEAEGLFSKLFSRSKPPRGLYVYGDVGT
  GKTMVMDMFYAYVEMKRKKRVHFHGFMLDVHKRIHRLKQSLPKRKPGFMAKSYDPIAPIAEEISEEACLL
  CFDEFQVTDIADAMILKQLFENLFKNGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCNTVQLDSG
  IDYRKRELPAAGKLYYLTSEADVEAVMDKLFDELAQKQNDLTRPRILKVQGRELRLNKACGTVADCTFEE
  LCERPLGASDYLELSKNFDTIFLRNIPQFTLANRTQGRRFITLIDNFYDLKVRIICSASTPISSLFLHQH
  HDSELEQSRILMDDLGLSQDSAEGLSMFTGEEEIFAFQRTISRLTEMQTEQYWNEGDRTKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145315
• ORF Size: 1443 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145315.5
• RefSeq Size: 2262 bp
• RefSeq ORF: 1446 bp
• Locus ID: 246269
• UniProt ID: Q8WV93
• Cytogenetics: 6q21
• MW: 54.8 kDa
• Gene Summary: This gene encodes a mitochondrial integral membrane protein that plays a role in mitochondrial protein homeostasis. The protein contains a P-loop motif and a five-domain structure that is conserved in fly, yeast, and bacteria. It functions to mediate the degradation of nuclear-encoded complex IV subunits. Two conserved estrogen receptor binding sites are located within 2.5 kb of this gene. Polymorphisms in this gene have been associated with bipolar disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2016]