Apoptosis enhancing nuclease (AEN) (NM_022767) Human Tagged ORF Clone

CAT#: RC202285

AEN (Myc-DDK-tagged)-Human apoptosis enhancing nuclease (AEN)

Product Images

Circular map for RC202285

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  Western blot validation of overexpression lysate (Cat# LY402942) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202285 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AEN protein (Cat# TP302285). The protein was produced from HEK293T cells transfected with AEN cDNA clone (Cat# RC202285) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apoptosis enhancing nuclease
• Synonyms: ISG20L1; pp12744
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202285 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTACCCCGGGAGGCCCCTGAGTCTGCTCAGTGCCTGTGCCCTTCCCTCACCATCCCAAATGCCAAGG
  ATGTGCTTCGGAAGAGGCACAAGAGAAGGAGCCGACAGCACCAGCGGTTCATGGCCCGGAAGGCCTTGCT
  GCAGGAGCAGGGGCTGCTGAGCATGCCTCCAGAACCAGGGTCCTCCCCACTGCCCACCCCTTTCGGGGCA
  GCGACAGCAACTGAAGCTGCCAGCAGTGGGAAGCAGTGTCTGAGGGCTGGATCTGGCAGTGCCCCATGCA
  GCAGAAGGCCTGCTCCCGGGAAAGCCTCAGGGCCCTTGCCCAGCAAGTGTGTGGCTATCGACTGTGAGAT
  GGTGGGCACGGGACCCCGAGGGCGGGTAAGCGAGCTGGCCCGCTGTTCCATTGTGAGCTACCATGGCGAT
  GTCCTCTATGACAAGTACATCAGGCCTGAGATGCCCATCGCTGACTACCGTACCCGCTGGAGTGGCATCA
  CTCGGCAGCACATGCGCAAGGCTGTCCCCTTCCAGGTGGCCCAGAAAGAGATCCTTAAGCTCCTGAAGGG
  CAAGGTGGTGGTGGGGCACGCGCTGCACAACGACTTCCAGGCGCTCAAGTATGTCCACCCTCGGAGCCAG
  ACCCGGGATACCACCTATGTCCCAAACTTCCTCAGCGAGCCCGGCCTCCACACCCGGGCCCGGGTCTCTC
  TAAAGGACCTGGCCCTGCAGCTGCTGCACAAGAAGATCCAGGTGGGCCAGCACGGGCACTCATCAGTAGA
  AGATGCCACGACAGCCATGGAGCTCTACCGGCTGGTGGAGGTGCAGTGGGAACAGCAGGAGGCCCGCAGC
  CTCTGGACCTGCCCCGAGGACAGAGAACCTGACAGCAGCACAGACATGGAACAGTACATGGAGGACCAGT
  ACTGGCCCGATGACCTGGCCCACGGCAGCAGAGGAGGAGCCAGGGAGGCACAGGACAGAAGGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202285 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVPREAPESAQCLCPSLTIPNAKDVLRKRHKRRSRQHQRFMARKALLQEQGLLSMPPEPGSSPLPTPFGA
  ATATEAASSGKQCLRAGSGSAPCSRRPAPGKASGPLPSKCVAIDCEMVGTGPRGRVSELARCSIVSYHGD
  VLYDKYIRPEMPIADYRTRWSGITRQHMRKAVPFQVAQKEILKLLKGKVVVGHALHNDFQALKYVHPRSQ
  TRDTTYVPNFLSEPGLHTRARVSLKDLALQLLHKKIQVGQHGHSSVEDATTAMELYRLVEVQWEQQEARS
  LWTCPEDREPDSSTDMEQYMEDQYWPDDLAHGSRGGAREAQDRRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022767
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022767.2, NP_073604.2
• RefSeq Size: 3134 bp
• RefSeq ORF: 978 bp
• Locus ID: 64782
• UniProt ID: Q8WTP8
• Cytogenetics: 15q26.1
• Domains: EXOIII
• MW: 36.4 kDa
• Gene Summary: Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis. When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNA damage signals, leading to enhancement of apoptosis.[UniProtKB/Swiss-Prot Function]