C14orf133 (VIPAS39) (NM_022067) Human Tagged ORF Clone

CAT#: RC202264

VIPAS39 (Myc-DDK-tagged)-Human VPS33B interacting protein, apical-basolateral polarity regulator (VIPAR), transcript variant 2

Product Images

Circular map for RC202264

• 

  Western blot validation of overexpression lysate (Cat# LY402902) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202264 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C14orf133
• Synonyms: C14orf133; hSPE-39; SPE-39; SPE39; VIPAR; VPS16B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202264 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCGGACAAAGGGTGATGAGGAGGAGTATTGGAACAGCTCCAAGTTCAAGGCTTTTACCTTTGACG
  ATGAAGACGATGAGCTTTCACAGTTAAAGGAGTCCAAGCGGGCGGTGAACAGCCTCCGAGACTTCGTGGA
  TGATGATGACGATGATGACCTGGAGCGAGTCAGCTGGAGTGGGGAACCTGTGGGAAGTATCTCATGGTCC
  ATCAGAGAGACTGCTGGTAATAGCGGCTCAACCCACGAGGGGCGTGAACAGCTAAAGAGCCGAAACAGCT
  TCTCCTCCTATGCACAACTACCCAAGCCTACTTCTACCTACTCCCTGAGCAGCTTTTTTAGAGGTAGAAC
  TAGACCTGGAAGTTTCCAGTCCCTTTCTGATGCTCTGTCAGACACACCTGCCAAAAGCTATGCTCCAGAG
  CTGGGGAGACCCAAAGGGGAGTATAGGGATTACAGCAATGACTGGAGCCCCAGTGATACAGTGCGACGTC
  TCCGGAAGGGCAAGGTTTGCTCACTAGAGAGATTCCGCTCCTTACAGGACAAACTACAACTCCTAGAAGA
  GGCAGTAAGCATGCATGATGGAAACGTCATTACTGCAGTTCTGATTTTCCTGAAGAGGACACTGAGCAAA
  GAGATCCTCTTCCGAGAGCTGGAGGTGCGACAGGTTGCCCTGAGACATCTTATTCACTTCCTTAAGGAAA
  TAGGGGATCAAAAGTTGCTTTTAGACCTCTTCAGGTTCCTAGATAGAACAGAAGAGCTTGCGCTATCTCA
  TTATCGAGAGCATTTGAACATTCAGGACCCTGACAAACGAAAAGAATTTCTTAAAACCTGTGTTGGTTTG
  CCATTTTCAGCAGAAGATTCCGCACACATACAGGACCATTACACGCTCCTGGAACGTCAGATCATTATTG
  AGGCAAATGATCGCCATCTAGAATCAGCAGGACAGACTGAGATCTTCCGAAAGCACCCCCGCAAAGCCTC
  CATCCTCAACATGCCACTAGTGACAACACTTTTCTACTCCTGCTTCTATCACTACACAGAGGCTGAGGGG
  ACATTCAGCAGTCCCGTCAACCTGAAGAAGACATTTAAGATCCCAGATAAACAGTATGTGCTGACAGCCC
  TGGCTGCTCGTGCCAAGCTTCGAGCCTGGAATGATGTAGATGCCCTATTCACCACAAAGAACTGGCTGGG
  CTATACCAAGAAGAGAGCACCCATTGGCTTCCATCGGGTTGTCGAAATTTTGCACAAGAACAATGCCCCT
  GTGCAGATATTACAGGAGTATGTCAATCTGGTGGAAGATGTGGACACGAAGTTGAACTTAGCCACTAAGT
  TCAAGTGCCATGATGTCGTCATTGATACCTACCGGGACCTGAAGGATCGTCAACAGTTGCTAGCATACAG
  GAGTAAGGTAGATAAAGGATCAGCAGAGGAGGAGAAGATTGATGCTCTTCTCAGCAGCTCGCAAATTCGA
  TGGAAGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202264 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNRTKGDEEEYWNSSKFKAFTFDDEDDELSQLKESKRAVNSLRDFVDDDDDDDLERVSWSGEPVGSISWS
  IRETAGNSGSTHEGREQLKSRNSFSSYAQLPKPTSTYSLSSFFRGRTRPGSFQSLSDALSDTPAKSYAPE
  LGRPKGEYRDYSNDWSPSDTVRRLRKGKVCSLERFRSLQDKLQLLEEAVSMHDGNVITAVLIFLKRTLSK
  EILFRELEVRQVALRHLIHFLKEIGDQKLLLDLFRFLDRTEELALSHYREHLNIQDPDKRKEFLKTCVGL
  PFSAEDSAHIQDHYTLLERQIIIEANDRHLESAGQTEIFRKHPRKASILNMPLVTTLFYSCFYHYTEAEG
  TFSSPVNLKKTFKIPDKQYVLTALAARAKLRAWNDVDALFTTKNWLGYTKKRAPIGFHRVVEILHKNNAP
  VQILQEYVNLVEDVDTKLNLATKFKCHDVVIDTYRDLKDRQQLLAYRSKVDKGSAEEEKIDALLSSSQIR
  WKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022067
• ORF Size: 1479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022067.4
• RefSeq Size: 2889 bp
• RefSeq ORF: 1482 bp
• Locus ID: 63894
• UniProt ID: Q9H9C1
• Cytogenetics: 14q24.3
• MW: 57 kDa
• Gene Summary: This gene encodes a protein involved in the sorting of lysosomal proteins. Mutations in this gene are associated with ARCS2 (arthrogryposis, renal dysfunction, and cholestasis-2). Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010]