PEX11A (NM_003847) Human Tagged ORF Clone

CAT#: RC202096

PEX11A (Myc-DDK-tagged)-Human peroxisomal biogenesis factor 11 alpha (PEX11A)

Product Images

Circular map for RC202096

• 

  Western blot validation of overexpression lysate (Cat# LY401264) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202096 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PEX11A
• Synonyms: hsPEX11p; PEX11-ALPHA; PMP28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202096 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCCTTCACCCGCTTCACCAACCAGACCCAGGGCCGGGACCGACTCTTCAGAGCCACTCAGTACA
  CATGCATGTTGCTTAGATATTTGTTAGAGCCCAAAGCTGGCAAAGAGAAGGTGGTAATGAAGCTCAAGAA
  ACTGGAGTCCAGTGTGAGCACTGGTCGTAAATGGTTCAGACTAGGCAATGTGGTACATGCTATACAGGCA
  ACTGAGCAGAGCATTCATGCCACTGACCTGGTACCTCGCTTATGCTTAACATTAGCCAACCTGAACCGTG
  TGATTTATTTCATCTGTGACACCATCCTCTGGGTGAGGAGCGTAGGTCTCACCTCTGGCATCAACAAAGA
  GAAATGGCGAACGAGGGCTGCTCACCACTACTACTATTCTCTTCTGCTGAGCCTTGTCAGGGATCTGTAT
  GAAATCTCCCTGCAGATGAAACGAGTTACATGTGACAGGGCAAAGAAAGAGAAATCAGCATCCCAGGATC
  CTCTTTGGTTCAGCGTGGCTGAGGAGGAAACAGAATGGCTCCAATCCTTTCTACTTCTTTTATTCCGATC
  TCTGAAGCAGCATCCTCCCTTGCTCCTGGACACAGTGAAGAACCTTTGTGATATCCTGAACCCTTTGGAC
  CAGCTGGGGATCTATAAATCCAATCCTGGCATCATTGGACTTGGAGGTCTTGTGTCCTCTATAGCAGGCA
  TGATCACTGTGGCATATCCTCAGATGAAGCTGAAGACCCGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202096 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDAFTRFTNQTQGRDRLFRATQYTCMLLRYLLEPKAGKEKVVMKLKKLESSVSTGRKWFRLGNVVHAIQA
  TEQSIHATDLVPRLCLTLANLNRVIYFICDTILWVRSVGLTSGINKEKWRTRAAHHYYYSLLLSLVRDLY
  EISLQMKRVTCDRAKKEKSASQDPLWFSVAEEETEWLQSFLLLLFRSLKQHPPLLLDTVKNLCDILNPLD
  QLGIYKSNPGIIGLGGLVSSIAGMITVAYPQMKLKTR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003847
• ORF Size: 741 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003847.1, NP_003838.1
• RefSeq Size: 2742 bp
• RefSeq ORF: 744 bp
• Locus ID: 8800
• UniProt ID: O75192
• Cytogenetics: 15q26.1
• Protein Families: Transmembrane
• MW: 28.4 kDa
• Gene Summary: This gene is a member of the PEX11 family, which is composed of membrane elongation factors involved in regulation of peroxisome maintenance and proliferation. This gene product interacts with peroxisomal membrane protein 19 and may respond to outside stimuli to increase peroxisome abundance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012]