GABA A Receptor beta 3 (GABRB3) (NM_000814) Human Tagged ORF Clone

CAT#: RC201993

GABRB3 (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, beta 3 (GABRB3), transcript variant 1

Product Images

Circular map for RC201993

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  Western blot validation of overexpression lysate (Cat# LY400288) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201993 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GABA A Receptor beta 3
• Synonyms: DEE43; ECA5; EIEE43
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201993 representing NM_000814.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGGGGCCTTGCGGGAGGAAGGCTTTTCGGCATCTTCTCGGCCCCGGTGCTGGTGGCTGTGGTGTGC
  TGCGCCCAGAGTGTGAACGATCCCGGGAACATGTCCTTTGTGAAGGAGACGGTGGACAAGCTGTTGAAA
  GGCTACGACATTCGCCTAAGACCCGACTTCGGGGGTCCCCCGGTCTGCGTGGGGATGAACATCGACATC
  GCCAGCATCGACATGGTTTCCGAAGTCAACATGGATTATACCTTAACCATGTATTTTCAACAATATTGG
  AGAGATAAAAGGCTCGCCTATTCTGGGATCCCTCTCAACCTCACGCTTGACAATCGAGTGGCTGACCAG
  CTATGGGTGCCCGACACATATTTCTTAAATGACAAAAAGTCATTTGTGCATGGAGTGACAGTGAAAAAC
  CGCATGATCCGTCTTCACCCTGATGGGACAGTGCTGTATGGGCTCAGAATCACCACGACAGCAGCATGC
  ATGATGGACCTCAGGAGATACCCCCTGGACGAGCAGAACTGCACTCTGGAAATTGAAAGCTATGGCTAC
  ACCACGGATGACATTGAGTTTTACTGGCGAGGCGGGGACAAGGCTGTTACCGGAGTGGAAAGGATTGAG
  CTCCCGCAGTTCTCCATCGTGGAGCACCGTCTGGTCTCGAGGAATGTTGTCTTCGCCACAGGTGCCTAT
  CCTCGACTGTCACTGAGCTTTCGGTTGAAGAGGAACATTGGATACTTCATTCTTCAGACTTATATGCCC
  TCTATACTGATAACGATTCTGTCGTGGGTGTCCTTCTGGATCAATTATGATGCATCTGCTGCTAGAGTT
  GCCCTCGGGATCACAACTGTGCTGACAATGACAACCATCAACACCCACCTTCGGGAGACCTTGCCCAAA
  ATCCCCTATGTCAAAGCCATTGACATGTACCTTATGGGCTGCTTCGTCTTTGTGTTCCTGGCCCTTCTG
  GAGTATGCCTTTGTCAACTACATTTTCTTTGGAAGAGGCCCTCAAAGGCAGAAGAAGCTTGCAGAAAAG
  ACAGCCAAGGCAAAGAATGACCGTTCAAAGAGCGAAAGCAACCGGGTGGATGCTCATGGAAATATTCTG
  TTGACATCGCTGGAAGTTCACAATGAAATGAATGAGGTCTCAGGCGGCATTGGCGATACCAGGAATTCA
  GCAATATCCTTTGACAACTCAGGAATCCAGTACAGGAAACAGAGCATGCCTCGAGAAGGGCATGGGCGA
  TTCCTGGGGGACAGAAGCCTCCCGCACAAGAAGACCCATCTACGGAGGAGGTCTTCACAGCTCAAAATT
  AAAATACCTGATCTAACCGATGTGAATGCCATAGACAGATGGTCCAGGATCGTGTTTCCATTCACTTTT
  TCTCTTTTCAACTTAGTTTACTGGCTGTACTATGTTAAC
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC201993
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MWGLAGGRLFGIFSAPVLVAVVCCAQSVNDPGNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDI
  ASIDMVSEVNMDYTLTMYFQQYWRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKN
  RMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVERIE
  LPQFSIVEHRLVSRNVVFATGAYPRLSLSFRLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARV
  ALGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFLALLEYAFVNYIFFGRGPQRQKKLAEK
  TAKAKNDRSKSESNRVDAHGNILLTSLEVHNEMNEVSGGIGDTRNSAISFDNSGIQYRKQSMPREGHGR
  FLGDRSLPHKKTHLRRRSSQLKIKIPDLTDVNAIDRWSRIVFPFTFSLFNLVYWLYYVN
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000814
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_000814.6
• RefSeq Size: 5811 bp
• RefSeq ORF: 1422 bp
• Locus ID: 2562
• UniProt ID: P28472
• Cytogenetics: 15q12
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 54.1 kDa
• Gene Summary: This gene encodes a member of the ligand-gated ionic channel family. The encoded protein is one the subunits of a multi-subunit chloride channel that serves as the receptor for gamma-aminobutyric acid, a major inhibitory neurotransmitter of the mammalian nervous system. This gene is located on the long arm of chromosome 15 in a cluster with two other genes encoding related subunits of the family. This gene may be associated with the pathogenesis of several disorders including Angelman syndrome, Prader-Willi syndrome, nonsyndromic orofacial clefts, epilepsy and autism. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013]