GABA A Receptor beta 3 (GABRB3) (NM_000814) Human Untagged Clone

CAT#: SC125324

GABRB3 (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, beta 3 (GABRB3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GABA A Receptor beta 3
• Synonyms: DEE43; ECA5; EIEE43
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125324 sequence for NM_000814 edited (data generated by NextGen Sequencing)
  ATGTGGGGCCTTGCGGGAGGAAGGCTTTTCGGCATCTTCTCGGCCCCGGTGCTGGTGGCT
  GTGGTGTGCTGCGCCCAGAGTGTGAACGATCCCGGGAACATGTCCTTTGTGAAGGAGACG
  GTGGACAAGCTGTTGAAAGGCTACGACATTCGCCTAAGACCCGACTTCGGGGGTCCCCCG
  GTCTGCGTGGGGATGAACATCGACATCGCCAGCATCGACATGGTTTCCGAAGTCAACATG
  GATTATACCTTAACCATGTATTTTCAACAATATTGGAGAGATAAAAGGCTCGCCTATTCT
  GGGATCCCTCTCAACCTCACGCTTGACAATCGAGTGGCTGACCAGCTATGGGTGCCCGAC
  ACATATTTCTTAAATGACAAAAAGTCATTTGTGCATGGAGTGACAGTGAAAAACCGCATG
  ATCCGTCTTCACCCTGATGGGACAGTGCTGTATGGGCTCAGAATCACCACGACAGCAGCA
  TGCATGATGGACCTCAGGAGATACCCCCTGGACGAGCAGAACTGCACTCTGGAAATTGAA
  AGCTATGGCTACACCACGGATGACATTGAGTTTTACTGGCGAGGCGGGGACAAGGCTGTT
  ACCGGAGTGGAAAGGATTGAGCTCCCGCAGTTCTCCATCGTGGAGCACCGTCTGGTCTCG
  AGGAATGTTGTCTTCGCCACAGGTGCCTATCCTCGACTGTCACTGAGCTTTCGGTTGAAG
  AGGAACATTGGATACTTCATTCTTCAGACTTATATGCCCTCTATACTGATAACGATTCTG
  TCGTGGGTGTCCTTCTGGATCAATTATGATGCATCTGCTGCTAGAGTTGCCCTCGGGATC
  ACAACTGTGCTGACAATGACAACCATCAACACCCACCTTCGGGAGACCTTGCCCAAAATC
  CCCTATGTCAAAGCCATTGACATGTACCTTATGGGCTGCTTCGTCTTTGTGTTCCTGGCC
  CTTCTGGAGTATGCCTTTGTCAACTACATTTTCTTTGGAAGAGGCCCTCAAAGGCAGAAG
  AAGCTTGCAGAAAAGACAGCCAAGGCAAAGAATGACCGTTCAAAGAGCGAAAGCAACCGG
  GTGGATGCTCATGGAAATATTCTGTTGACATCGCTGGAAGTTCACAATGAAATGAATGAG
  GTCTCAGGCGGCATTGGCGATACCAGGAATTCAGCAATATCCTTTGACAACTCAGGAATC
  CAGTACAGGAAACAGAGCATGCCTCGAGAAGGGCATGGGCGATTCCTGGGGGACAGAAGC
  CTCCCGCACAAGAAGACCCATCTACGGAGGAGGTCTTCACAGCTCAAAATTAAAATACCT
  GATCTAACCGATGTGAATGCCATAGACAGATGGTCCAGGATCGTGTTTCCATTCACTTTT
  TCTCTTTTCAACTTAGTTTACTGGCTGTACTATGTTAACTGA
  
  Clone variation with respect to NM_000814.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000814 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCTGCGGGTCGCGACGGC
  GGCGGGGCGCCCCCTCCCCCGTGCCGGGGCGCGGCGGAGGGATGTGGGGCCTTGCGGGAG
  GAAGGCTTTTCGGCATCTTCTCGGCCCCGGTGCTGGTGGCTGTGGTGTGCTGCGCCCAGA
  GTGTGAACGATCCCGGGAACATGTCCTTTGTGAAGGAGACGGTGGACAAGCTGTTGAAAG
  GCTACGACATTCGCCTAAGACCCGACTTCGGGGGTCCCCCGGTCTGCGTGGGGATGAACA
  TCGACATCGCCAGCATCGACATGGTTTCCGAAGTCAACATGGATTATACCTTAACCATGT
  ATTTTCAACAATATTGGAGAGATAAAAGGCTCGCCTATTCTGGGATCCCTCTCAACCTCA
  CGCTTGACAATCGAGTGGCTGACCACTATGGGTGCCCGACACATATTTCTTAAATGACAA
  AAAGTCATTTGTGCATGGAGTGACAGTGAAAAACCGCATGATCCGTCTTACCCTGATGGG
  ACAGTGCTGTATGGGCTCAGAATCACCACGACAGCAGCATGCATGATGGACCTCAGGAGA
  TACCCCCTGGACGAGCAGAACTGCACTCTGGANATTGAAAGCTATGGCTACCCACGGATG
  ACATTTGAGTTTTACTGCGGAGGCGGNACNAAGGCTGTACCCGGAGTGGAAAAGGATTGA
  CTCCCCNGCAGTTTTCCATCGTGGACACCCGTCTGTCTCNAGGGAAATGTTGTCTTCGCC
  ACAGGTGCCATCCCTCACTGACTGNNNNTTTTTTGGGTGGAGAGGACATTGGATACTTCA
  TTCTTAGACTTATTGCCTCTTCTGTACATCTTGNGGGGGGGGGGTGCCCTTCGGAAACAT
  TATGAGCATTGTGCCAAAGTGCCCCTCGATACACACTGGCTGACATGCACATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000814 unedited
  ACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTAGAAAGATCTCATCTAAATAGTAA
  GTTAAGGTTGCGTCTATGAAACCGGTGCACCTTGTCTTGTTTCTACGAGCTTTAAAAAGT
  CCAAATACTGATGAGGCTGTGGATCAGAAGGAACCAACAGTGACTGTGTTTGCTGTCAGG
  TTGCCCTCCTTTGCCTCAGAGAACGGTCATGGGTCGTTGGGTGACGCGTGGTGTGTACGG
  GTCATGTTCCTGCAGAATCCTAGTCAGTCTAAGAGTAGTTGGATGTGCTTATGAAATATG
  TCAGATACAGAAATAAAGGCATGAGGATGATTCTTAGTTTCTGAAATTGAACAGTAGAAG
  TGTTTTAGCTTCAATCTTAATTTCTAGATAACAGAAAGTAGTTACATCACATAAAGGAAA
  ATATGACATCGCATGCTGCCATGATAGCAAGCGTAAGAAACAAAATCTTGGTAAATATAC
  ATATATATGCATATTATGCAAATATATTTTCTATTAACATACAATGTTATAAGAATGATA
  ACATAACTGCAAGGGGGAAAATCATCCTAGATGGTTTTACCTTAGAGTAACGAATAACCT
  GAGACGTCTATGCTTTCTGTTGGATATCANGCCTAGAAATCCATACGAGATGTAGAGCAC
  ATTTTCAGTGATTGAAATGTCACTGGCCAAACGTGTCACTGTGTTCGCACATGACAGTGA
  AGCTGGATCCACTCCNNAGTGACAACAGATACAAACTACACACTGTATATTAGAGTACTT
  ACAACGAGATGCCATTCACTCCCGATGAGTTTTCAAAAGAGGTCGTTCAGACTTCACGTG
  GGATCTATCTTGNTTCAGACACAGGTATCACACTGCCGTGGCTCACCCACCACTAACCAT
  GCAGAAACGTTGCCCGAGAAACAAACCCTCCCAGAAAGCCAGAAGCCTTGCTACTAACTG
  ACGAAGGAATTGATAAGGCCCCTTTAACAGCATACCTGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000814
• Insert Size: 1422 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000814.3, NP_000805.1
• RefSeq Size: 3053 bp
• RefSeq ORF: 1422 bp
• Locus ID: 2562
• UniProt ID: P28472
• Cytogenetics: 15q12
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a member of the ligand-gated ionic channel family. The encoded protein is one the subunits of a multi-subunit chloride channel that serves as the receptor for gamma-aminobutyric acid, a major inhibitory neurotransmitter of the mammalian nervous system. This gene is located on the long arm of chromosome 15 in a cluster with two other genes encoding related subunits of the family. This gene may be associated with the pathogenesis of several disorders including Angelman syndrome, Prader-Willi syndrome, nonsyndromic orofacial clefts, epilepsy and autism. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) encodes isoform 1, which is of the same length as isoform 2, but has a distinct N-terminus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.