TRIM31 (NM_007028) Human Tagged ORF Clone

CAT#: RC201930

TRIM31 (Myc-DDK-tagged)-Human tripartite motif containing 31 (TRIM31)

Product Images

Circular map for RC201930

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  Western blot validation of overexpression lysate (Cat# LY416247) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201930 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TRIM31 protein (Cat# TP301930). The protein was produced from HEK293T cells transfected with TRIM31 cDNA clone (Cat# RC201930) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIM31
• Synonyms: C6orf13; HCG1; HCGI; RNF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201930 representing NM_007028
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGTGGGCAGTTTGTGAACAAACTGCAAGAGGAAGTGATCTGCCCCATCTGCCTGGACATTCTGC
  AGAAACCTGTCACCATCGACTGTGGGCACAATTTCTGCCTCAAATGCATCACTCAGATTGGGGAAACATC
  ATGTGGATTTTTCAAATGTCCCCTCTGCAAAACTTCCGTAAGGAAGAACGCAATCAGGTTCAACTCGCTG
  TTGCGGAATCTGGTGGAGAAAATCCAAGCTCTACAAGCCTCTGAGGTGCAGTCCAAAAGGAAAGAGGCTA
  CATGCCCGAGGCACCAGGAGATGTTCCACTATTTCTGCGAGGATGATGGGAAGTTCCTCTGTTTTGTGTG
  TCGTGAATCCAAGGACCACAAATCCCATAATGTCAGCTTGATCGAAGAAGCTGCCCAGAATTATCAGGGG
  CAGATTCAAGAGCAGATCCAAGTCTTGCAGCAAAAGGAGAAGGAGACAGTACAAGTGAAGGCACAAGGTG
  TACACAGGGTCGATGTCTTCACGGACCAGGTAGAACATGAGAAGCAAAGGATCCTCACAGAATTTGAACT
  CCTGCATCAAGTCCTAGAGGAGGAGAAGAATTTCCTGCTATCACGGATTTACTGGCTGGGTCATGAGGGA
  ACGGAAGCGGGGAAACACTATGTTGCCTCCACTGAGCCACAGTTGAACGATCTCAAGAAGCTCGTTGATT
  CCCTGAAGACCAAGCAGAACATGCCACCCAGGCAGCTGCTGGAGGATATCAAAGTCGTCTTGTGCAGAAG
  TGAAGAGTTTCAGTTTCTCAACCCAACCCCTGTTCCTCTGGAACTGGAGAAAAAACTCAGTGAAGCAAAA
  TCAAGACACGACTCCATCACAGGGAGCCTGAAAAAATTCAAAGACCAACTCCAGGCTGATAGGAAAAAAG
  ATGAAAACAGATTCTTCAAAAGCATGAATAAAAATGACATGAAGAGCTGGGGCTTGTTACAGAAAAATAA
  TCATAAAATGAACAAAACCTCAGAGCCCGGGTCATCTTCTGCAGGCGGCAGAACTACATCGGGGCCACCA
  AATCACCACTCTTCAGCCCCATCCCACTCCCTGTTTCGGGCCTCGTCTGCTGGGAAAGTCACTTTTCCAG
  TATGTCTCCTGGCCTCTTATGATGAGATTTCTGGTCAAGGAGCGAGCTCTCAGGATACGAAGACATTTGA
  CGTTGCGCTGTCCGAGGAGCTCCATGCGGCACTGAGTGAGTGGCTGACAGCGATCCGGGCTTGGTTTTGT
  AAGGTTCCTTCAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201930 representing NM_007028
  Red=Cloning site Green=Tags(s)
  
  MASGQFVNKLQEEVICPICLDILQKPVTIDCGHNFCLKCITQIGETSCGFFKCPLCKTSVRKNAIRFNSL
  LRNLVEKIQALQASEVQSKRKEATCPRHQEMFHYFCEDDGKFLCFVCRESKDHKSHNVSLIEEAAQNYQG
  QIQEQIQVLQQKEKETVQVKAQGVHRVDVFTDQVEHEKQRILTEFELLHQVLEEEKNFLLSRIYWLGHEG
  TEAGKHYVASTEPQLNDLKKLVDSLKTKQNMPPRQLLEDIKVVLCRSEEFQFLNPTPVPLELEKKLSEAK
  SRHDSITGSLKKFKDQLQADRKKDENRFFKSMNKNDMKSWGLLQKNNHKMNKTSEPGSSSAGGRTTSGPP
  NHHSSAPSHSLFRASSAGKVTFPVCLLASYDEISGQGASSQDTKTFDVALSEELHAALSEWLTAIRAWFC
  KVPSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007028
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007028.5
• RefSeq Size: 2049 bp
• RefSeq ORF: 1278 bp
• Locus ID: 11074
• UniProt ID: Q9BZY9
• Cytogenetics: 6p22.1
• MW: 48.1 kDa
• Gene Summary: This gene encodes a protein that functions as an E3 ubiquitin-protein ligase. This gene shows altered expression in certain tumors and may be a negative regulator of cell growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]