RAB7 (RAB7A) (NM_004637) Human Tagged ORF Clone
RAB7A (Myc-DDK-tagged)-Human RAB7A, member RAS oncogene family (RAB7A)
|Type||Human Tagged ORF Clone|
|Synonyms||CMT2B; PRO2706; RAB7|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC201776 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC201776 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||621 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2240 bp|
|RefSeq ORF||624 bp|
|Protein Families||Druggable Genome|
|Gene Summary||RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RC201776L1||Lenti ORF clone of Human RAB7A, member RAS oncogene family (RAB7A), Myc-DDK-tagged||
|RC201776L2||Lenti ORF clone of Human RAB7A, member RAS oncogene family (RAB7A), mGFP tagged||
|RC201776L3||Lenti ORF clone of Human RAB7A, member RAS oncogene family (RAB7A), Myc-DDK-tagged||
|RC201776L4||Lenti ORF clone of Human RAB7A, member RAS oncogene family (RAB7A), mGFP tagged||
|RG201776||RAB7A (GFP-tagged) - Human RAB7A, member RAS oncogene family (RAB7A)||
|SC117240||RAB7A (untagged)-Human RAB7A, member RAS oncogene family (RAB7A)||