Importin 13 (IPO13) (NM_014652) Human Tagged ORF Clone

CAT#: RC201577

IPO13 (Myc-DDK-tagged)-Human importin 13 (IPO13)

Product Images

Circular map for RC201577

• 

  Western blot validation of overexpression lysate (Cat# LY402359) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201577 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Importin 13
• Synonyms: IMP13; KAP13; LGL2; RANBP13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201577 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCGGGAGGAGCAGCCGGGGGCTGCAGGGGCTGGAGCAGCACCAGCCTTGGACTTCACTGTGG
  AGAACGTGGAGAAGGCGCTGCACCAGCTCTACTATGATCCCAACATTGAGAATAAGAACCTGGCTCAGAA
  GTGGCTGATGCAGGCCCAGGTCTCCCCACAGGCCTGGCACTTCAGCTGGCAGCTACTGCAGCCCGACAAG
  GTACCAGAGATCCAGTACTTTGGGGCCAGTGCTCTTCACATCAAGATCTCTCGCTACTGGAGTGACATCC
  CCACTGACCAGTATGAAAGCCTAAAGGCACAGCTCTTCACCCAGATCACCCGCTTTGCCAGTGGCTCCAA
  GATTGTACTGACTCGGCTGTGCGTGGCACTGGCCTCACTGGCTCTCAGCATGATGCCTGATGCTTGGCCA
  TGTGCTGTGGCAGATATGGTACGACTCTTCCAGGCTGAGGACTCACCAGTGGATGGGCAGGGCCGCTGCC
  TAGCCCTGTTAGAGCTGCTGACAGTGCTGCCTGAGGAGTTCCAGACCAGTCGCCTACCCCAGTACCGCAA
  AGGCCTGGTGCGGACCAGCCTGGCGGTGGAATGTGGGGCTGTCTTCCCGCTGCTGGAGCAGCTGCTACAG
  CAGCCCAGCTCACCCAGCTGTGTGCGTCAGAAGGTGCTCAAGTGTTTCTCCAGCTGGGTGCAGCTGGAGG
  TGCCGCTGCAGGACTGTGAGGCGCTCATTCAGGCTGCCTTTGCTGCTCTGCAGGACTCGGAGCTCTTCGA
  CAGCAGTGTGGAGGCCATTGTGAATGCCATCTCACAGCCTGATGCCCAGAGGTACGTGAACACACTCCTG
  AAACTCATCCCGCTGGTGCTGGGTCTGCAGGAACAACTGCGGCAGGCAGTGCAGAATGGGGACATGGAGA
  CCTCCCATGGCATCTGTCGCATCGCTGTGGCCCTGGGCGAGAACCACTCCCGGGCCTTGCTGGACCAAGT
  AGAGCACTGGCAGAGTTTCCTGGCACTCGTCAACATGATTATGTTCTGCACAGGCATCCCTGGCCACTAT
  CCTGTCAATGAGACCACCAGCTCCCTAACCCTCACCTTCTGGTACACACTGCAGGATGATATTCTATCCT
  TTGAGGCAGAGAAGCAGGCTGTATACCAGCAGGTGTACCGGCCAGTCTACTTCCAGCTGGTGGATGTGCT
  TCTGCACAAGGCCCAGTTCCCTTCTGATGAGGAATATGGATTCTGGTCCTCAGACGAGAAGGAGCAGTTC
  CGAATTTACAGGGTGGACATCTCAGACACGCTCATGTATGTCTATGAGATGTTGGGGGCCGAGCTGCTCA
  GCAACCTCTATGACAAGCTGGGTCGTTTGCTCACCAGCTCAGAGGAGCCCTACTCCTGGCAGCACACAGA
  GGCCCTCCTCTACGGCTTCCAATCCATCGCAGAGACCATTGACGTCAACTATTCTGATGTGGTGCCTGGG
  CTCATTGGCCTCATCCCACGGATCAGCATCAGCAACGTGCAGCTGGCAGACACTGTCATGTTCACCATTG
  GAGCTCTGTCTGAATGGCTGGCTGACCACCCCGTCATGATCAACAGTGTTCTGCCCTTGGTACTGCATGC
  CCTAGGCAATCCTGAGCTGTCTGTCTCTTCTGTGTCCACCCTCAAGAAGATCTGCCGAGAGTGCAAGTAT
  GACCTGCCTCCCTATGCTGCCAACATTGTGGCTGTGTCCCAGGATGTGCTGATGAAACAGATCCACAAGA
  CAAGCCAGTGCATGTGGCTGATGCAGGCGCTGGGCTTCCTGCTGTCAGCTCTTCAAGTGGAGGAGATCCT
  TAAGAACCTGCACTCGCTTATCTCACCCTATATCCAGCAACTGGAGAAGCTGGCAGAGGAGATACCCAAT
  CCCTCCAACAAGCTGGCCATTGTTCACATCTTGGGGCTTCTCTCCAACCTCTTCACCACACTGGACATCA
  GTCATCATGAGGATGATCATGAAGGCCCTGAGCTTCGGAAGCTGCCAGTGCCACAGGGACCCAACCCCGT
  GGTGGTGGTGCTGCAGCAGGTCTTCCAGCTTATCCAGAAGGTGCTGAGCAAATGGTTGAATGATGCCCAG
  GTTGTGGAGGCGGTGTGCGCTATCTTTGAGAAGTCTGTTAAGACGCTGCTGGATGACTTTGCCCCCATGG
  TGCCACAGCTGTGTGAGATGCTGGGTCGGATGTACAGCACCATCCCCCAGGCCTCTGCTCTTGACCTCAC
  TCGACAGCTGGTCCACATCTTTGCTCATGAGCCTGCCCACTTTCCCCCAATTGAGGCCCTCTTCCTGCTC
  GTCACCTCCGTCACACTCACTCTCTTCCAGCAAGGGCCCAGGGATCATCCTGATATTGTTGATTCATTTA
  TGCAACTCCTGGCACAGGCTCTGAAGCGGAAGCCAGATTTGTTCCTGTGTGAACGATTGGATGTCAAATC
  TGTGTTCCAGTGTGCTGTGCTGGCCCTCAAGTTCCCTGAGGCACCTACTGTCAAGGCCTCCTGTGGCTTC
  TTTACAGAGCTGCTGCCTCGGTGTGGGGAAGTAGAGTCTGTGGGAAAGGTGGTACAGGAAGACGGTCGTA
  TGCTGCTCATAGCAGTGCTGGAGGCCATTGGGGGCCAGGCCTCCCGCAGCCTCATGGACTGCTTTGCCGA
  TATCCTGTTCGCCCTGAACAAGCACTGCTTCAGCCTCCTGAGCATGTGGATCAAGGAGGCCCTGCAGCCA
  CCTGGTTTCCCCTCTGCCCGCCTCAGCCCTGAACAGAAGGATACCTTCAGCCAGCAGATCCTTCGCGAGC
  GAGTGAACAAGAGGCGGGTGAAGGAGATGGTGAAGGAGTTCACACTGCTGTGCCGGGGTCTCCATGGCAC
  AGATTACACAGCTGACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201577 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERREEQPGAAGAGAAPALDFTVENVEKALHQLYYDPNIENKNLAQKWLMQAQVSPQAWHFSWQLLQPDK
  VPEIQYFGASALHIKISRYWSDIPTDQYESLKAQLFTQITRFASGSKIVLTRLCVALASLALSMMPDAWP
  CAVADMVRLFQAEDSPVDGQGRCLALLELLTVLPEEFQTSRLPQYRKGLVRTSLAVECGAVFPLLEQLLQ
  QPSSPSCVRQKVLKCFSSWVQLEVPLQDCEALIQAAFAALQDSELFDSSVEAIVNAISQPDAQRYVNTLL
  KLIPLVLGLQEQLRQAVQNGDMETSHGICRIAVALGENHSRALLDQVEHWQSFLALVNMIMFCTGIPGHY
  PVNETTSSLTLTFWYTLQDDILSFEAEKQAVYQQVYRPVYFQLVDVLLHKAQFPSDEEYGFWSSDEKEQF
  RIYRVDISDTLMYVYEMLGAELLSNLYDKLGRLLTSSEEPYSWQHTEALLYGFQSIAETIDVNYSDVVPG
  LIGLIPRISISNVQLADTVMFTIGALSEWLADHPVMINSVLPLVLHALGNPELSVSSVSTLKKICRECKY
  DLPPYAANIVAVSQDVLMKQIHKTSQCMWLMQALGFLLSALQVEEILKNLHSLISPYIQQLEKLAEEIPN
  PSNKLAIVHILGLLSNLFTTLDISHHEDDHEGPELRKLPVPQGPNPVVVVLQQVFQLIQKVLSKWLNDAQ
  VVEAVCAIFEKSVKTLLDDFAPMVPQLCEMLGRMYSTIPQASALDLTRQLVHIFAHEPAHFPPIEALFLL
  VTSVTLTLFQQGPRDHPDIVDSFMQLLAQALKRKPDLFLCERLDVKSVFQCAVLALKFPEAPTVKASCGF
  FTELLPRCGEVESVGKVVQEDGRMLLIAVLEAIGGQASRSLMDCFADILFALNKHCFSLLSMWIKEALQP
  PGFPSARLSPEQKDTFSQQILRERVNKRRVKEMVKEFTLLCRGLHGTDYTADY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014652
• ORF Size: 2889 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014652.2, NP_055467.2
• RefSeq Size: 4041 bp
• RefSeq ORF: 2892 bp
• Locus ID: 9670
• UniProt ID: O94829
• Cytogenetics: 1p34.1
• Domains: IBN_NT
• MW: 108.2 kDa
• Gene Summary: This gene encodes a member of the importin-beta family of nuclear transport proteins. The encoded protein mediates the import of specific cargo proteins from the cytoplasm to the nucleus and is dependent on the Ras-related nuclear protein-GTPase system. The encoded protein is also involved in nuclear export of the eukaryotic translation initiation factor 1A.[provided by RefSeq, Mar 2009]