TTC4 (NM_004623) Human Tagged ORF Clone

CAT#: RC201570

TTC4 (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 4 (TTC4)

Product Images

Circular map for RC201570

• 

  Western blot validation of overexpression lysate (Cat# LY417865) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201570 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TTC4
• Synonyms: CNS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201570 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACAACCTGGGCAGGATCCCACCTCAGACGACGTCATGGACTCGTTCCTGGAAAAGTTCCAGAGCC
  AGCCTTACCGTGGCGGCTTTCATGAGGACCAGTGGGAGAAGGAATTTGAAAAGGTCCCCCTATTTATGAC
  GAGAGCGCCATCAGAAATTGATCCCAGGGAGAATCCTGACTTGGCTTGTCTCCAGTCAATTATTTTTGAT
  GAGGAGCGTTCTCCAGAAGAACAGGCCAAGACCTATAAAGATGAGGGCAATGATTACTTTAAAGAAAAAG
  ACTACAAGAAAGCTGTAATTTCATACACTGAAGGCTTAAAGAAGAAATGTGCAGATCCTGATTTGAATGC
  TGTCCTTTATACCAACCGGGCAGCAGCACAGTACTATCTGGGCAATTTTCGTTCTGCTCTCAATGATGTG
  ACAGCTGCCAGAAAGCTAAAACCCTGCCACCTCAAAGCAATAATAAGAGGTGCCTTATGCCATCTGGAAC
  TGAAACACTTTGCCGAGGCCGTGAACTGGTGTGATGAGGGACTGCAAATAGATGCCAAAGAGAAGAAGCT
  TCTGGAAATGAGGGCTAAAGCAGACAAGCTGAAGCGAATTGAACAGAGGGATGTGAGGAAAGCCAACTTG
  AAAGAAAAGAAGGAGAGGAATCAGAATGAGGCTTTACTCCAGGCCATCAAGGCTAGGAATATCAGGCTCT
  CAGAAGCTGCCTGTGAGGATGAAGATTCAGCCTCAGAAGGTCTAGGTGAGCTTTTCCTGGATGGACTCAG
  CACTGAGAACCCCCATGGAGCCAGGCTGAGTCTAGATGGCCAGGGCAGGCTGAGCTGGCCTGTGCTCTTT
  CTGTACCCAGAGTATGCCCAGTCGGACTTCATCTCTGCTTTTCATGAGGACTCCAGGTTTATTGATCATC
  TAATGGTGATGTTTGGTGAAACACCCTCTTGGGACCTAGAGCAAAAATATTGCCCTGATAATTTGGAGGT
  CTACTTTGAGGATGAGGACAGGGCAGAACTATACCGGGTGCCTGCCAAGAGCACCTTGCTACAGGTTCTA
  CAGCACCAGAGGTACTTTGTAAAAGCCCTGACACCAGCATTTTTGGTCTGTGTAGGATCCTCTCCTTTTT
  GCAAGAATTTTCTCCGGGGGAGAAAGGTGTACCAGATACGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201570 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEQPGQDPTSDDVMDSFLEKFQSQPYRGGFHEDQWEKEFEKVPLFMTRAPSEIDPRENPDLACLQSIIFD
  EERSPEEQAKTYKDEGNDYFKEKDYKKAVISYTEGLKKKCADPDLNAVLYTNRAAAQYYLGNFRSALNDV
  TAARKLKPCHLKAIIRGALCHLELKHFAEAVNWCDEGLQIDAKEKKLLEMRAKADKLKRIEQRDVRKANL
  KEKKERNQNEALLQAIKARNIRLSEAACEDEDSASEGLGELFLDGLSTENPHGARLSLDGQGRLSWPVLF
  LYPEYAQSDFISAFHEDSRFIDHLMVMFGETPSWDLEQKYCPDNLEVYFEDEDRAELYRVPAKSTLLQVL
  QHQRYFVKALTPAFLVCVGSSPFCKNFLRGRKVYQIR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004623
• ORF Size: 1161 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004623.2, NP_004614.2
• RefSeq Size: 2395 bp
• RefSeq ORF: 1164 bp
• Locus ID: 7268
• UniProt ID: O95801
• Cytogenetics: 1p32.3
• Domains: TPR
• MW: 44.7 kDa
• Gene Summary: This gene encodes a protein that contains tetratricopeptide (TPR) repeats, which often mediate protein-protein interactions and chaperone activity. The encoded protein interacts with heat shock proteins 70 and 90. Alternative splicing results in multiple transcript variants. Naturally-occuring readthrough transcription occurs from upstream gene MROH (maestro heat-like repeat family member 7) to this gene. [provided by RefSeq, Apr 2014]