IFRD1 (NM_001007245) Human Tagged ORF Clone

CAT#: RC201560

IFRD1 (Myc-DDK-tagged)-Human interferon-related developmental regulator 1 (IFRD1), transcript variant 2

Product Images

Circular map for RC201560

• 

  Western blot validation of overexpression lysate (Cat# LY419866) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217538 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IFRD1
• Synonyms: PC4; TIS7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201560 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAAGAACAAGAAGCGGAACACTCCCCACCGCGGTAGCAGTGCTGGCGGCGGCGGGTCAGGAGCAG
  CCGCAGCGACGGCGGCGACAGCAGGTGGCCAGCATCGAAATGTTCAGCCTTTTAGTGATGAAGATGCATC
  AATTGAAACAATGAGCCATTGCAGTGGTTATAGCGATCCTTCCAGTTTTGCTGAAGATGGACCAGAAGTC
  CTTGATGAGGAAGGAACTCAAGAAGACCTAGAGTACAAGTTGAAGGGATTAATTGACCTAACCCTGGATA
  AGAGTGCGAAGACAAGGCAAGCAGCTCTTGAAGGTATTAAAAATGCACTGGCTTCAAAAATGCTGTATGA
  ATTTATTCTGGAAAGGAGAATGACTTTAACTGATAGCATTGAACGCTGCCTGAAAAAAGGTAAGAGTGAT
  GAGCAACGTGCAGCTGCAGCGTTAGCATCTGTTCTTTGTATTCAGCTGGGCCCTGGAATTGAAAGTGAAG
  AGATTTTGAAAACTCTTGGACCAATCCTAAAGAAAATCATTTGTGATGGGTCAGCTAGTATGCAGGCTAG
  GCAAACTTGTGCAACTTGCTTTGGTGTTTGCTGTTTTATTGCCACAGATGACATTACTGAACTATACTCA
  ACTCTGGAATGTTTGGAAAATATCTTCACTAAATCCTATCTCAAAGAGAAAGACACTACTGTTATTTGCA
  GCACTCCTAATACAGTGCTTCATATCAGCTCTCTTCTTGCATGGACACTACTGCTGACCATATGCCCAAT
  CAATGAAGTGAAGAAAAAGCTTGAGATGCATTTCCATAAGCTTCCAAGCCTCCTCTCTTGTGATGATGTA
  AACATGAGAATAGCTGCTGGTGAATCTTTGGCACTTCTCTTTGAATTGGCCAGAGGAATAGAGAGTGACT
  TTTTTTATGAAGACATGGAGTCCTTGACGCAGATGCTTAGGGCCTTGGCAACAGATGGAAATAAACACCG
  GGCCAAAGTGGACAAGAGAAAGCAGCGGTCAGTTTTCAGAGATGTCCTGAGGGCAGTGGAGGAACGGGAT
  TTTCCAACAGAAACCATTAAATTTGGTCCTGAACGCATGTATATTGATTGCTGGGTAAAAAAACACACCT
  ATGACACCTTTAAGGAGGTTCTTGGATCAGGGATGCAGTACCACTTGCAGTCAAATGAATTCCTTCGAAA
  TGTATTTGAACTTGGACCCCCAGTGATGCTTGATGCTGCAACGCTTAAAACGATGAAGATTTCTCGTTTC
  GAAAGGCATTTATATAACTCTGCAGCCTTCAAAGCTCGAACCAAAGCTAGAAGCAAATGTCGAGATAAGA
  GAGCAGATGTTGGAGAATTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201560 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPKNKKRNTPHRGSSAGGGGSGAAAATAATAGGQHRNVQPFSDEDASIETMSHCSGYSDPSSFAEDGPEV
  LDEEGTQEDLEYKLKGLIDLTLDKSAKTRQAALEGIKNALASKMLYEFILERRMTLTDSIERCLKKGKSD
  EQRAAAALASVLCIQLGPGIESEEILKTLGPILKKIICDGSASMQARQTCATCFGVCCFIATDDITELYS
  TLECLENIFTKSYLKEKDTTVICSTPNTVLHISSLLAWTLLLTICPINEVKKKLEMHFHKLPSLLSCDDV
  NMRIAAGESLALLFELARGIESDFFYEDMESLTQMLRALATDGNKHRAKVDKRKQRSVFRDVLRAVEERD
  FPTETIKFGPERMYIDCWVKKHTYDTFKEVLGSGMQYHLQSNEFLRNVFELGPPVMLDAATLKTMKISRF
  ERHLYNSAAFKARTKARSKCRDKRADVGEFF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007245
• ORF Size: 1353 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007245.2
• RefSeq Size: 3514 bp
• RefSeq ORF: 1356 bp
• Locus ID: 3475
• UniProt ID: O00458
• Cytogenetics: 7q31.1
• MW: 50.3 kDa
• Gene Summary: This gene is an immediate early gene that encodes a protein related to interferon-gamma. This protein may function as a transcriptional co-activator/repressor that controls the growth and differentiation of specific cell types during embryonic development and tissue regeneration. Mutations in this gene are associated with sensory/motor neuropathy with ataxia. This gene may also be involved in modulating the pathogenesis of cystic fibrosis lung disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2010]