TRIM9 (NM_052978) Human Tagged ORF Clone

CAT#: RC201423

TRIM9 (Myc-DDK-tagged)-Human tripartite motif containing 9 (TRIM9), transcript variant 2

Product Images

Circular map for RC201423

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TRIM9 (Cat# RC201423, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM9(Cat# TA800044). Positive lysates LY409342 (100ug) and LC409342 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY409342) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201423 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIM9
• Synonyms: RNF91; SPRING
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201423 representing NM_052978
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGATGGAAGAGGAGTTGAAATGCCCCGTGTGCGGCTCCTTCTATCGGGAGCCCATCATCCTGC
  CCTGCTCTCACAATTTGTGTCAGGCGTGCGCCCGCAACATCCTGGTGCAGACCCCAGAGTCTGAATCCCC
  CCAGAGCCATCGGGCCGCGGGCTCCGGGGTCTCCGACTATGACTATCTGGACCTGGACAAGATGAGCCTA
  TACAGCGAGGCGGACAGCGGCTATGGCTCCTACGGGGGGTTCGCCAGCGCCCCCACTACCCCGTGCCAGA
  AGTCCCCCAACGGCGTCCGCGTGTTTCCCCCGGCTATGCCGCCACCGGCCACCCACTTGTCACCGGCCCT
  GGCCCCGGTGCCCCGCAACTCCTGTATCACCTGCCCCCAGTGTCACCGCAGCCTCATCCTGGATGACCGG
  GGGCTCCGCGGCTTCCCCAAGAATCGCGTACTGGAAGGGGTAATTGACCGCTACCAGCAGAGCAAAGCCG
  CGGCCCTCAAGTGCCAGCTCTGCGAGAAGGCGCCCAAGGAAGCCACCGTCATGTGCGAACAGTGCGATGT
  CTTCTACTGCGATCCGTGCCGCCTGCGCTGCCACCCGCCCCGGGGGCCCCTAGCCAAGCACCGCCTGGTG
  CCCCCGGCCCAGGGTCGTGTGAGCCGGAGGCTGAGCCCACGCAAGGTCTCCACCTGCACAGACCACGAGC
  TGGAGAACCACAGCATGTACTGCGTGCAATGCAAGATGCCCGTGTGCTACCAGTGCTTGGAGGAGGGCAA
  ACACTCCAGCCACGAAGTCAAGGCTCTGGGGGCCATGTGGAAACTACATAAGAGCCAGCTCTCCCAGGCG
  CTGAACGGACTGTCAGACAGGGCCAAAGAAGCCAAGGAGTTTCTGGTACAGCTGCGCAACATGGTCCAGC
  AGATCCAGGAGAACAGTGTGGAGTTTGAAGCCTGTCTGGTGGCCCAATGTGATGCCCTCATCGATGCCCT
  CAACAGAAGAAAAGCCCAGCTGCTGGCCCGCGTCAACAAGGAGCATGAGCACAAGCTGAAGGTGGTTCGA
  GATCAGATCTCTCACTGCACAGTGAAATTGCGCCAGACCACAGGTCTCATGGAGTACTGCTTGGAGGTGA
  TTAAGGAAAATGATCCTAGTGGTTTTTTGCAGATTTCTGACGCCCTCATAAGAAGAGTGCACCTGACTGA
  GGATCAGTGGGGTAAAGGCACACTCACTCCAAGGATGACCACGGACTTTGACTTGAGTCTGGACAACAGC
  CCTCTGCTGCAATCCATCCACCAGCTGGATTTCGTGCAAGTGAAAGCTTCCTCTCCAGTCCCAGCAACCC
  CTATCCTACAGCTGGAGGAATGTTGTACCCACAACAACAGCGCTACGTTGTCCTGGAAACAGCCACCTCT
  GTCCACGGTGCCCGCCGATGGATACATTCTGGAGCTGGATGATGGCAACGGTGGTCAATTCCGGGAGGTG
  TATGTGGGGAAGGAGACAATGTGCACTGTGGATGGTCTTCACTTCAACAGCACATACAACGCTCGGGTCA
  AGGCCTTCAACAAAACAGGAGTCAGCCCGTACAGCAAGACCCTGGTCCTCCAAACGTCTGAGGGTAAGGC
  CCTTCAGCAGTATCCCTCAGAGCGAGAACTGCGTGGCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201423 representing NM_052978
  Red=Cloning site Green=Tags(s)
  
  MEEMEEELKCPVCGSFYREPIILPCSHNLCQACARNILVQTPESESPQSHRAAGSGVSDYDYLDLDKMSL
  YSEADSGYGSYGGFASAPTTPCQKSPNGVRVFPPAMPPPATHLSPALAPVPRNSCITCPQCHRSLILDDR
  GLRGFPKNRVLEGVIDRYQQSKAAALKCQLCEKAPKEATVMCEQCDVFYCDPCRLRCHPPRGPLAKHRLV
  PPAQGRVSRRLSPRKVSTCTDHELENHSMYCVQCKMPVCYQCLEEGKHSSHEVKALGAMWKLHKSQLSQA
  LNGLSDRAKEAKEFLVQLRNMVQQIQENSVEFEACLVAQCDALIDALNRRKAQLLARVNKEHEHKLKVVR
  DQISHCTVKLRQTTGLMEYCLEVIKENDPSGFLQISDALIRRVHLTEDQWGKGTLTPRMTTDFDLSLDNS
  PLLQSIHQLDFVQVKASSPVPATPILQLEECCTHNNSATLSWKQPPLSTVPADGYILELDDGNGGQFREV
  YVGKETMCTVDGLHFNSTYNARVKAFNKTGVSPYSKTLVLQTSEGKALQQYPSERELRGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052978
• ORF Size: 1650 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052978.4
• RefSeq Size: 4017 bp
• RefSeq ORF: 1653 bp
• Locus ID: 114088
• UniProt ID: Q9C026
• Cytogenetics: 14q22.1
• Domains: zf-B_box, BBC, FN3
• Protein Families: Druggable Genome
• MW: 61.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]