NPDC1 (NM_015392) Human Tagged ORF Clone

CAT#: RC201415

NPDC1 (Myc-DDK-tagged)-Human neural proliferation, differentiation and control, 1 (NPDC1)

Product Images

Circular map for RC201415

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  Western blot validation of overexpression lysate (Cat# LY414555) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201415 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NPDC1 protein (Cat# TP301415). The protein was produced from HEK293T cells transfected with NPDC1 cDNA clone (Cat# RC201415) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NPDC1
• Synonyms: CAB; CAB-; CAB-1; CAB1; NPDC-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201415 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGCCGCTGCCTCCGCCCTCCCCGCGGCACCTGCGGCTGCTGCGGCTGCTGCTCTCCGGCCTCG
  TCCTCGGCGCCGCCCTGCGTGGAGCCGCCGCCGGCCACCCGGATGTAGCCGCCTGTCCCGGGAGCCTGGA
  CTGTGCCCTGAAGAGGCGGGCAAGGTGTCCTCCTGGTGCACATGCCTGTGGGCCCTGCCTTCAGCCCTTC
  CAGGAGGACCAGCAAGGGCTCTGTGTGCCCAGGATGCGCCGGCCTCCAGGCGGGGGCCGGCCCCAGCCCA
  GACTGGAAGATGAGATTGACTTCCTGGCCCAGGAGCTTGCCCGGAAGGAGTCTGGACACTCAACTCCGCC
  CCTACCCAAGGACCGACAGCGGCTCCCGGAGCCTGCCACCCTGGGCTTCTCGGCACGGGGGCAGGGGCTG
  GAGCTGGGCCTCCCCTCCACTCCAGGAACCCCCACGCCCACGCCCCACACCTCCCTGGGCTCCCCTGTGT
  CATCCGACCCGGTGCACATGTCGCCCCTGGAGCCCCGGGGAGGGCAAGGCGACGGCCTCGCCCTTGTGCT
  GATCCTGGCGTTCTGTGTGGCCGGTGCAGCCGCCCTCTCCGTAGCCTCCCTCTGCTGGTGCAGGCTGCAG
  CGTGAGATCCGCCTGACTCAGAAGGCCGACTACGCCACTGCGAAGGCCCCTGGCTCACCTGCAGCTCCCC
  GGATCTCGCCTGGGGACCAGCGGCTGGCACAGAGCGCGGAGATGTACCACTACCAGCACCAACGGCAACA
  GATGCTGTGCCTGGAGCGGCATAAAGAGCCACCCAAGGAGCTGGACACGGCCTCCTCGGATGAGGAGAAT
  GAGGACGGAGACTTCACGGTGTACGAGTGCCCGGGCCTGGCCCCGACCGGGGAAATGGAGGTGCGCAACC
  CTCTGTTCGACCACGCCGCACTGTCCGCGCCCCTGCCGGCCCCCAGCTCACCGCCTGCACTGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201415 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATPLPPPSPRHLRLLRLLLSGLVLGAALRGAAAGHPDVAACPGSLDCALKRRARCPPGAHACGPCLQPF
  QEDQQGLCVPRMRRPPGGGRPQPRLEDEIDFLAQELARKESGHSTPPLPKDRQRLPEPATLGFSARGQGL
  ELGLPSTPGTPTPTPHTSLGSPVSSDPVHMSPLEPRGGQGDGLALVLILAFCVAGAAALSVASLCWCRLQ
  REIRLTQKADYATAKAPGSPAAPRISPGDQRLAQSAEMYHYQHQRQQMLCLERHKEPPKELDTASSDEEN
  EDGDFTVYECPGLAPTGEMEVRNPLFDHAALSAPLPAPSSPPALP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015392
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015392.4
• RefSeq Size: 1548 bp
• RefSeq ORF: 978 bp
• Locus ID: 56654
• UniProt ID: Q9NQX5
• Cytogenetics: 9q34.3
• Protein Families: Transmembrane
• MW: 34.5 kDa
• Gene Summary: Suppresses oncogenic transformation in neural and non-neural cells and down-regulates neural cell proliferation. Might be involved in transcriptional regulation (By similarity).[UniProtKB/Swiss-Prot Function]