NALP2 (NLRP2) (NM_017852) Human Tagged ORF Clone

CAT#: RC201357

NLRP2 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 2 (NLRP2), transcript variant 1

Product Images

Circular map for RC201357

• 

  Western blot validation of overexpression lysate (Cat# LY413416) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201357 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NLRP2 protein (Cat# TP301357). The protein was produced from HEK293T cells transfected with NLRP2 cDNA clone (Cat# RC201357) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NALP2
• Synonyms: CLR19.9; NALP2; NBS1; PAN1; PYPAF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201357 representing NM_017852
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCTTCGGCGCAGATGGGCTTCAACCTGCAGGCTCTCCTGGAGCAGCTCAGCCAGGATGAGTTGA
  GCAAGTTCAAGTATCTGATCACGACCTTCTCCCTGGCACACGAGCTCCAGAAGATCCCCCACAAGGAGGT
  AGACAAGGCTGATGGGAAGCAACTGGTAGAAATCCTCACCACCCATTGTGACAGCTACTGGGTGGAGATG
  GCGAGCCTCCAGGTCTTTGAAAAGATGCACCGAATGGATCTGTCTGAGAGAGCAAAGGATGAAGTCAGAG
  AAGCAGCTTTGAAATCCTTTAATAAAAGGAAACCTCTATCATTAGGGATAACACGGAAAGAACGACCACC
  TCTAGACGTGGACGAAATGCTGGAGCGCTTCAAAACAGAAGCACAAGCGTTTACAGAAACGAAAGGAAAT
  GTCATCTGCCTGGGTAAAGAAGTCTTTAAAGGAAAAAAGCCAGACAAAGACAATAGGTGCAGGTATATAT
  TGAAGACGAAGTTCCGGGAGATGTGGAAGAGCTGGCCTGGAGATAGCAAAGAGGTCCAGGTTATGGCTGA
  GAGATACAAGATGCTGATCCCATTCAGCAACCCCAGGGTGCTTCCCGGGCCCTTCTCATACACGGTGGTG
  CTGTATGGTCCTGCAGGCCTTGGGAAAACCACGCTGGCCCAGAAACTAATGCTAGACTGGGCAGAGGACA
  ACCTCATCCACAAATTCAAATATGCGTTCTACCTCAGCTGCAGGGAGCTCAGCCGCCTGGGCCCGTGCAG
  TTTTGCAGAGCTGGTCTTCAGGGACTGGCCTGAATTGCAGGATGACATTCCACACATCCTAGCCCAAGCA
  CGGAAAATCTTGTTCGTGATTGACGGCTTTGATGAGCTGGGAGCCGCACCTGGGGCGCTGATCGAGGACA
  TCTGCGGGGACTGGGAGAAGAAGAAGCCGGTGCCCGTCCTCCTGGGGAGTTTGCTGAACAGGGTGATGTT
  ACCCAAGGCCGCCCTGCTGGTCACCACGCGGCCCAGGGCCCTGAGGGACCTCCGGATCCTGGCGGAGGAG
  CCGATCTACATAAGGGTGGAGGGCTTCCTGGAGGAGGACAGGAGGGCCTATTTCCTGAGACACTTTGGAG
  ACGAGGACCAAGCCATGCGTGCCTTTGAGCTAATGAGGAGCAACGCGGCCCTGTTCCAGCTGGGCTCGGC
  CCCCGCGGTGTGCTGGATCGTGTGCACGACTCTGAAGCTGCAGATGGAGAAGGGGGAGGACCCGGTCCCC
  ACCTGCCTCACCCGCACGGGGCTGTTCCTGCGTTTCCTCTGCAGCCGGTTCCCGCAGGGCGCACAGCTGC
  GGGGCGCGCTGCGGACGCTGAGCCTCCTGGCCGCGCAGGGCCTGTGGGCGCAGACGTCCGTGCTTCACCG
  AGAGGATCTGGAAAGGCTCGGGGTGCAGGAGTCCGACCTCCGTCTGTTCCTGGACGGAGACATCCTCCGC
  CAGGACAGAGTCTCCAAAGGCTGCTACTCCTTCATCCACCTCAGCTTCCAGCAGTTTCTCACTGCCCTGT
  TCTACACCCTGGAGAAGGAGGAGGAAGAGGATAGGGACGGCCACACCTGGGACATTGGGGACGTACAGAA
  GCTGCTTTCCGGAGTAGAAAGACTCAGGAACCCCGACCTGATCCAAGCAGGCTACTACTCCTTTGGCCTC
  GCTAACGAGAAGAGAGCCAAGGAGTTGGAGGCCACTTTTGGCTGCCGGATGTCACCGGACATCAAACAGG
  AATTGCTGCGATGCGACATAAGTTGTAAGGGTGGACATTCAACGGTGACAGACCTGCAGGAGCTCCTCGG
  CTGTCTGTACGAGTCTCAGGAGGAGGAGCTGGTGAAGGAGGTGATGGCTCAGTTCAAAGAAATATCCCTG
  CACTTAAATGCAGTAGACGTTGTGCCATCTTCATTCTGCGTCAAGCACTGTCGAAACCTGCAGAAAATGT
  CACTGCAGGTAATAAAGGAGAATCTCCCGGAGAATGTCACTGCGTCTGAATCAGACGCCGAGGTTGAGAG
  ATCCCAGGATGATCAGCACATGCTTCCTTTCTGGACGGACCTTTGTTCCATATTTGGATCAAATAAGGAT
  CTGATGGGTCTAGCAATCAATGATAGCTTTCTCAGTGCCTCCCTAGTAAGGATCCTGTGTGAACAAATAG
  CCTCTGACACCTGTCATCTCCAGAGAGTGGTGTTCAAAAACATTTCCCCAGCTGATGCTCATCGGAACCT
  CTGCCTAGCTCTTCGAGGTCACAAGACTGTAACGTATCTGACCCTTCAAGGCAATGACCAGGATGATATG
  TTTCCCGCATTGTGTGAGGTCTTGAGACATCCAGAATGTAACCTGCGATATCTCGGGTTGGTGTCTTGTT
  CCGCTACCACTCAGCAGTGGGCTGATCTCTCCTTGGCCCTTGAAGTCAACCAGTCCCTGACGTGCGTAAA
  CCTCTCCGACAATGAGCTTCTGGATGAGGGTGCTAAGTTGCTGTACACAACTTTGAGACACCCCAAGTGC
  TTTCTGCAGAGGTTGTCGTTGGAAAACTGTCACCTTACAGAAGCCAATTGCAAGGACCTTGCTGCTGTGT
  TGGTTGTCAGCCGGGAGCTGACACACCTGTGCTTGGCCAAGAACCCCATTGGGAATACAGGGGTGAAGTT
  TCTGTGTGAGGGCTTGAGGTACCCCGAGTGTAAACTGCAGACCTTGGTGCTTTGGAACTGCGACATAACT
  AGCGATGGCTGCTGCGATCTCACAAAGCTTCTCCAAGAAAAATCAAGCCTGTTGTGTTTGGATCTGGGGC
  TGAATCACATAGGAGTTAAGGGAATGAAGTTCCTGTGTGAGGCTTTGAGGAAACCACTGTGCAACTTGAG
  ATGTCTGTGGTTGTGGGGATGTTCCATCCCTCCGTTCAGTTGTGAAGACCTCTGCTCTGCCCTCAGCTGC
  AACCAGAGCCTCGTCACTCTGGACCTGGGTCAGAATCCCTTGGGGTCTAGTGGAGTGAAGATGCTGTTTG
  AAACCTTGACATGTTCCAGTGGCACCCTCCGGACACTCAGGTTGAAAATAGATGACTTTAATGATGAACT
  CAATAAGCTGCTGGAAGAAATAGAAGAAAAAAACCCACAACTGATTATTGATACTGAGAAACATCATCCC
  TGGGCAGAAAGGCCTTCTTCTCATGACTTCATGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201357 representing NM_017852
  Red=Cloning site Green=Tags(s)
  
  MVSSAQMGFNLQALLEQLSQDELSKFKYLITTFSLAHELQKIPHKEVDKADGKQLVEILTTHCDSYWVEM
  ASLQVFEKMHRMDLSERAKDEVREAALKSFNKRKPLSLGITRKERPPLDVDEMLERFKTEAQAFTETKGN
  VICLGKEVFKGKKPDKDNRCRYILKTKFREMWKSWPGDSKEVQVMAERYKMLIPFSNPRVLPGPFSYTVV
  LYGPAGLGKTTLAQKLMLDWAEDNLIHKFKYAFYLSCRELSRLGPCSFAELVFRDWPELQDDIPHILAQA
  RKILFVIDGFDELGAAPGALIEDICGDWEKKKPVPVLLGSLLNRVMLPKAALLVTTRPRALRDLRILAEE
  PIYIRVEGFLEEDRRAYFLRHFGDEDQAMRAFELMRSNAALFQLGSAPAVCWIVCTTLKLQMEKGEDPVP
  TCLTRTGLFLRFLCSRFPQGAQLRGALRTLSLLAAQGLWAQTSVLHREDLERLGVQESDLRLFLDGDILR
  QDRVSKGCYSFIHLSFQQFLTALFYTLEKEEEEDRDGHTWDIGDVQKLLSGVERLRNPDLIQAGYYSFGL
  ANEKRAKELEATFGCRMSPDIKQELLRCDISCKGGHSTVTDLQELLGCLYESQEEELVKEVMAQFKEISL
  HLNAVDVVPSSFCVKHCRNLQKMSLQVIKENLPENVTASESDAEVERSQDDQHMLPFWTDLCSIFGSNKD
  LMGLAINDSFLSASLVRILCEQIASDTCHLQRVVFKNISPADAHRNLCLALRGHKTVTYLTLQGNDQDDM
  FPALCEVLRHPECNLRYLGLVSCSATTQQWADLSLALEVNQSLTCVNLSDNELLDEGAKLLYTTLRHPKC
  FLQRLSLENCHLTEANCKDLAAVLVVSRELTHLCLAKNPIGNTGVKFLCEGLRYPECKLQTLVLWNCDIT
  SDGCCDLTKLLQEKSSLLCLDLGLNHIGVKGMKFLCEALRKPLCNLRCLWLWGCSIPPFSCEDLCSALSC
  NQSLVTLDLGQNPLGSSGVKMLFETLTCSSGTLRTLRLKIDDFNDELNKLLEEIEEKNPQLIIDTEKHHP
  WAERPSSHDFMI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017852
• ORF Size: 3186 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017852.2
• RefSeq Size: 3531 bp
• RefSeq ORF: 3189 bp
• Locus ID: 55655
• UniProt ID: Q9NX02
• Cytogenetics: 19q13.42
• Domains: LRR, LRR_RI
• MW: 120.3 kDa
• Gene Summary: This gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). Members of this gene family are thought to be important regulators of immune responses. This gene product interacts with components of the IkB kinase (IKK) complex, and can regulate both caspase-1 and NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) activity. The pyrin domain is necessary and sufficient for suppression of NF-kB activity. An allelic variant (rs147585490) has been found that is incapable of blocking the transcriptional activity of NF-kB. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]