GTF2H1 (NM_005316) Human Tagged ORF Clone

CAT#: RC201341

GTF2H1 (Myc-DDK-tagged)-Human general transcription factor IIH, polypeptide 1, 62kDa (GTF2H1), transcript variant 1

Product Images

Circular map for RC201341

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  Western blot validation of overexpression lysate (Cat# LY428028) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227747 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GTF2H1 protein (Cat# TP301341). The protein was produced from HEK293T cells transfected with GTF2H1 cDNA clone (Cat# RC201341) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GTF2H1
• Synonyms: BTF2; P62; TFB1; TFIIH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201341 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACCTCATCTGAAGAAGTTTTGCTGATTGTAAAGAAAGTGCGTCAAAAGAAGCAGGATGGAGCTC
  TGTACCTCATGGCAGAAAGAATTGCTTGGGCACCTGAAGGCAAAGATAGATTTACAATCAGCCATATGTA
  TGCAGATATTAAATGCCAGAAAATTAGTCCAGAAGGAAAAGCTAAAATTCAGCTTCAGCTGGTCCTACAT
  GCAGGGGACACAACTAACTTCCATTTTTCCAATGAAAGCACAGCAGTGAAAGAGCGAGATGCAGTAAAAG
  ACCTTCTTCAGCAGCTGCTGCCCAAATTCAAGAGGAAAGCAAATAAAGAACTGGAAGAGAAGAACAGAAT
  GCTGCAAGAAGATCCTGTTTTGTTTCAGCTTTATAAAGACCTTGTTGTGAGTCAAGTGATCAGTGCTGAG
  GAATTCTGGGCCAATCGTTTAAATGTGAATGCAACAGATAGTTCTTCCACATCCAATCATAAGCAGGATG
  TTGGCATTTCTGCTGCATTTCTGGCTGATGTCCGGCCCCAAACTGATGGCTGTAACGGTCTAAGATATAA
  TTTAACTTCTGATATCATTGAGTCCATATTTAGGACCTATCCAGCAGTAAAAATGAAATATGCAGAAAAT
  GTTCCCCACAACATGACAGAGAAGGAATTCTGGACACGTTTTTTCCAGTCCCATTATTTTCACAGGGATC
  GGCTGAATACAGGGTCAAAGGATCTCTTTGCAGAATGTGCCAAAATAGATGAAAAAGGCCTAAAAACAAT
  GGTTTCATTAGGAGTGAAAAACCCACTACTAGATTTAACAGCTTTGGAAGATAAACCATTAGATGAGGGC
  TATGGCATTTCCTCTGTGCCATCTGCTTCCAATTCTAAATCCATAAAAGAGAATAGTAATGCTGCCATCA
  TCAAGAGATTTAACCATCACAGTGCCATGGTCCTGGCAGCTGGACTCAGAAAACAAGAAGCACAAAATGA
  ACAAACTAGTGAGCCCAGCAACATGGATGGAAATTCCGGAGATGCAGACTGCTTTCAGCCAGCAGTCAAA
  AGGGCGAAATTACAAGAGTCCATTGAATATGAAGACTTGGGGAAAAATAATTCTGTAAAAACGATTGCAC
  TAAACCTCAAGAAGTCAGATAGGTATTATCATGGTCCAACTCCAATCCAGTCACTACAGTATGCAACAAG
  TCAGGACATTATTAATTCTTTTCAAAGTATTAGACAAGAAATGGAAGCTTATACACCCAAGTTAACTCAG
  GTTCTCTCAAGTAGTGCTGCCAGTAGTACCATCACAGCACTGTCACCTGGAGGGGCACTTATGCAGGGAG
  GAACACAGCAAGCCATAAACCAGATGGTGCCAAATGATATTCAATCTGAATTGAAACACTTATATGTAGC
  TGTTGGAGAACTTCTACGACATTTCTGGTCCTGCTTTCCTGTTAATACGCCATTCCTAGAAGAAAAGGTA
  GTGAAAATGAAAAGTAATTTGGAACGATTCCAAGTTACGAAGCTCTGTCCATTCCAAGAAAAGATTCGGA
  GACAGTATTTAAGCACAAATTTGGTAAGTCACATAGAAGAGATGCTCCAGACAGCCTACAACAAGCTCCA
  CACATGGCAGTCACGGCGTCTGATGAAGAAAACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201341 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLH
  AGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAE
  EFWANRLNVNATDSSSTSNHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAEN
  VPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPLLDLTALEDKPLDEG
  YGISSVPSASNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQEAQNEQTSEPSNMDGNSGDADCFQPAVK
  RAKLQESIEYEDLGKNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQ
  VLSSSAASSTITALSPGGALMQGGTQQAINQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKV
  VKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHTWQSRRLMKKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005316
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005316.4
• RefSeq Size: 3308 bp
• RefSeq ORF: 1647 bp
• Locus ID: 2965
• UniProt ID: P32780
• Cytogenetics: 11p15.1
• Domains: BSD
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Basal transcription factors, Nucleotide excision repair
• MW: 62 kDa
• Gene Summary: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.[UniProtKB/Swiss-Prot Function]