POLR2C (NM_032940) Human Tagged ORF Clone
CAT#: RC201317
POLR2C (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide C, 33kDa (POLR2C)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_032940" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | POLR2C |
Synonyms | hRPB33; hsRPB3; RPB3; RPB31 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201317 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTACGCCAACCAGCCTACCGTGCGGATCACGGAGCTCACTGACGAGAATGTCAAGTTCATCATCG AGAACACCGACCTGGCGGTGGCCAATTCGATTCGGAGGGTCTTCATCGCTGAGGTTCCCATAATAGCCAT TGACTGGGTTCAGATTGATGCCAATTCCTCAGTTCTTCATGATGAATTCATTGCTCACAGGCTTGGATTA ATTCCCCTCATTAGTGATGACATTGTGGACAAGCTGCAGTACTCTCGGGACTGCACATGTGAGGAGTTCT GCCCCGAGTGCTCGGTGGAGTTCACCCTCGATGTGCGGTGCAATGAAGACCAGACGCGACATGTCACGTC TCGAGACCTCATCTCCAACAGCCCCCGGGTCATTCCGGTGACATCCCGGAACCGAGATAATGACCCCAAT GACTACGTGGAGCAGGATGACATCCTCATCGTCAAGTTGAGAAAGGGCCAGGAGCTGAGACTTCGAGCCT ATGCCAAAAAGGGCTTTGGCAAGGAGCATGCCAAGTGGAACCCTACTGCAGGGGTGGCTTTTGAATACGA TCCAGACAATGCCCTGAGGCACACAGTGTACCCCAAGCCCGAGGAATGGCCAAAGAGTGAGTACTCGGAG CTGGATGAGGATGAGTCGCAGGCTCCCTATGACCCCAACGGCAAGCCAGAAAGGTTTTACTACAATGTGG AGTCCTGTGGCTCTCTGCGTCCTGAAACCATTGTCCTGTCAGCCCTCTCAGGATTGAAGAAGAAACTGAG TGATTTACAAACTCAATTAAGCCACGAGATCCAGAGTGATGTGCTAACCATAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201317 protein sequence
Red=Cloning site Green=Tags(s) MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGL IPLISDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPN DYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTAGVAFEYDPDNALRHTVYPKPEEWPKSEYSE LDEDESQAPYDPNGKPERFYYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_032940 |
ORF Size | 825 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032940.3 |
RefSeq Size | 1822 bp |
RefSeq ORF | 828 bp |
Locus ID | 5432 |
UniProt ID | P19387 |
Cytogenetics | 16q21 |
Domains | RNA_pol_A_bac |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
MW | 31.4 kDa |
Gene Summary | This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201317L3 | Lenti ORF clone of Human polymerase (RNA) II (DNA directed) polypeptide C, 33kDa (POLR2C), Myc-DDK-tagged |
USD 600.00 |
|
RC201317L4 | Lenti ORF clone of Human polymerase (RNA) II (DNA directed) polypeptide C, 33kDa (POLR2C), mGFP tagged |
USD 600.00 |
|
RG201317 | POLR2C (tGFP-tagged) - Human polymerase (RNA) II (DNA directed) polypeptide C, 33kDa (POLR2C) |
USD 500.00 |
|
SC321402 | POLR2C (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide C, 33kDa (POLR2C) |
USD 300.00 |
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