TFIIF (GTF2F1) (NM_002096) Human Tagged ORF Clone

CAT#: RC201294

GTF2F1 (Myc-DDK-tagged)-Human general transcription factor IIF, polypeptide 1, 74kDa (GTF2F1)

Product Images

Circular map for RC201294

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GTF2F1 (Cat# RC201294, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2F1(Cat# TA503604). Positive lysates LY400767 (100ug) and LC400767 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400767) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201294 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GTF2F1 protein (Cat# TP301294). The protein was produced from HEK293T cells transfected with GTF2F1 cDNA clone (Cat# RC201294) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TFIIF
• Synonyms: BTF4; RAP74; TF2F1; TFIIF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201294 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCTAGGCCCTAGCAGCCAGAATGTCACTGAATACGTCGTTCGAGTTCCTAAGAATACAACCA
  AAAAATATAACATCATGGCTTTTAATGCAGCCGACAAAGTCAACTTTGCTACGTGGAATCAGGCTCGGCT
  GGAGCGGGACTTGAGCAACAAGAAAATCTACCAAGAGGAGGAGATGCCCGAATCGGGCGCGGGCAGTGAG
  TTCAACCGCAAGCTTCGGGAGGAGGCTCGGAGGAAGAAGTACGGCATCGTCCTCAAGGAGTTCCGGCCCG
  AGGACCAGCCCTGGCTGCTCCGGGTCAACGGCAAATCAGGCAGGAAGTTCAAGGGCATCAAGAAGGGAGG
  CGTAACAGAGAACACGTCCTACTACATCTTCACCCAGTGCCCCGACGGGGCCTTCGAGGCCTTCCCCGTG
  CACAACTGGTACAATTTCACACCGCTGGCCCGGCATCGCACGCTCACTGCCGAGGAGGCCGAGGAGGAGT
  GGGAGAGGAGGAACAAGGTGCTGAACCACTTCAGCATCATGCAGCAGCGGCGGCTCAAGGATCAGGACCA
  GGACGAGGATGAGGAGGAGAAGGAGAAACGTGGCCGCAGGAAGGCGAGCGAGCTGCGCATCCACGACCTG
  GAGGACGACCTGGAGATGTCGTCCGATGCCAGTGATGCCAGTGGTGAGGAGGGGGGCAGAGTCCCCAAGG
  CCAAGAAGAAGGCGCCGCTGGCCAAGGGCGGCAGGAAAAAGAAGAAGAAGAAGGGTTCAGACGACGAGGC
  CTTCGAGGACAGCGATGATGGGGACTTCGAGGGCCAAGAGGTAGACTACATGTCAGACGGCTCCAGTAGC
  TCCCAAGAAGAGCCTGAGAGCAAGGCCAAGGCGCCGCAGCAGGAGGAGGGGCCCAAGGGTGTCGATGAGC
  AGAGCGACAGTAGTGAGGAGAGTGAGGAGGAGAAGCCGCCTGAGGAGGACAAGGAGGAGGAGGAGGAGAA
  GAAGGCACCCACCCCGCAGGAGAAGAAGCGCAGGAAAGACAGCAGCGAGGAGTCGGACAGCTCAGAGGAG
  AGCGACATTGACAGCGAGGCCTCCTCAGCCCTCTTCATGGCGAAGAAGAAGACGCCACCCAAGAGAGAGC
  GGAAGCCGTCGGGAGGGAGCTCAAGGGGCAACAGCCGCCCAGGCACGCCCAGCGCAGAGGGTGGCAGCAC
  CTCCTCCACCCTGCGGGCGGCTGCCAGCAAACTCGAGCAAGGGAAGCGGGTGAGCGAGATGCCTGCAGCC
  AAGCGGTTGCGGCTGGACACGGGACCCCAGAGCCTGTCTGGGAAGTCGACACCCCAGCCACCATCAGGCA
  AGACAACACCCAACAGCGGCGACGTGCAGGTGACTGAGGATGCCGTGCGCCGCTACCTGACACGGAAGCC
  CATGACCACTAAGGACCTGCTGAAAAAGTTCCAGACCAAGAAGACAGGGCTGAGCAGCGAGCAGACAGTG
  AACGTGTTGGCCCAGATCCTCAAGCGACTCAACCCCGAGCGCAAGATGATCAACGACAAAATGCACTTCT
  CCCTCAAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201294 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALGPSSQNVTEYVVRVPKNTTKKYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEMPESGAGSE
  FNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPV
  HNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQRRLKDQDQDEDEEEKEKRGRRKASELRIHDL
  EDDLEMSSDASDASGEEGGRVPKAKKKAPLAKGGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGSSS
  SQEEPESKAKAPQQEEGPKGVDEQSDSSEESEEEKPPEEDKEEEEEKKAPTPQEKKRRKDSSEESDSSEE
  SDIDSEASSALFMAKKKTPPKRERKPSGGSSRGNSRPGTPSAEGGSTSSTLRAAASKLEQGKRVSEMPAA
  KRLRLDTGPQSLSGKSTPQPPSGKTTPNSGDVQVTEDAVRRYLTRKPMTTKDLLKKFQTKKTGLSSEQTV
  NVLAQILKRLNPERKMINDKMHFSLKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002096
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002096.3
• RefSeq Size: 2551 bp
• RefSeq ORF: 1554 bp
• Locus ID: 2962
• UniProt ID: P35269
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 58.2 kDa
• Gene Summary: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.[UniProtKB/Swiss-Prot Function]