CDC42EP3 (NM_006449) Human Tagged ORF Clone

CAT#: RC201069

CDC42EP3 (Myc-DDK-tagged)-Human CDC42 effector protein (Rho GTPase binding) 3 (CDC42EP3)

Product Images

Circular map for RC201069

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  Western blot validation of overexpression lysate (Cat# LY416634) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201069 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CDC42EP3 protein (Cat# TP301069). The protein was produced from HEK293T cells transfected with CDC42EP3 cDNA clone (Cat# RC201069) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDC42EP3
• Synonyms: BORG2; CEP3; UB1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201069 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCCAAGACCCCAATTTACCTGAAAGCAGCCAATAACAAGAAAGGAAAGAAATTTAAACTGAGGG
  ACATTCTGTCTCCTGATATGATCAGTCCCCCGCTTGGAGACTTTCGCCACACCATCCACATTGGCAAAGA
  GGGCCAGCACGATGTCTTTGGAGATATTTCCTTTCTTCAAGGGAACTACGAGCTTTTACCTGGAAACCAG
  GAGAAAGCACACCTGGGCCAGTTCCCTGGGCATAATGAGTTCTTCCGGGCCAACAGCACCTCGGACTCTG
  TGTTCACAGAAACGCCCTCCCCGGTGCTCAAAAATGCCATCTCCCTCCCGACCATTGGAGGATCCCAAGC
  TCTCATGTTGCCCTTATTGTCACCAGTGACATTTAATTCCAAACAGGAGTCCTTCGGGCCAGCAAAGCTG
  CCCAGGCTTAGCTGCGAGCCCGTCATGGAGGAAAAAGCTCAGGAGAAAAGCAGTCTGTTGGAGAATGGGA
  CAGTCCACCAGGGAGACACCTCGTGGGGCTCCAGCGGTTCTGCATCTCAGTCCAGCCAAGGCAGAGACAG
  CCACTCCTCCAGCCTGTCCGAACAGTACCCCGACTGGCCAGCCGAGGACATGTTTGACCATCCCACCCCA
  TGCGAGCTCATCAAGGGAAAGACTAAGTCAGAGGAGTCCCTCTCTGACCTTACAGGTTCCCTCCTCTCCC
  TGCAGCTTGATCTTGGGCCCTCACTTTTGGATGAGGTGCTGAATGTAATGGATAAAAATAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201069 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQGNYELLPGNQ
  EKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQALMLPLLSPVTFNSKQESFGPAKL
  PRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSASQSSQGRDSHSSSLSEQYPDWPAEDMFDHPTP
  CELIKGKTKSEESLSDLTGSLLSLQLDLGPSLLDEVLNVMDKNK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006449
• ORF Size: 762 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006449.5
• RefSeq Size: 5715 bp
• RefSeq ORF: 765 bp
• Locus ID: 10602
• UniProt ID: Q9UKI2
• Cytogenetics: 2p22.2
• Domains: PBD
• MW: 27.7 kDa
• Gene Summary: This gene encodes a member of a small family of guanosine triphosphate (GTP) metabolizing proteins that contain a CRIB (Cdc42, Rac interactive binding) domain. Members of this family of proteins act as effectors of CDC42 function. The encoded protein is involved in actin cytoskeleton re-organization during cell shape changes, including pseudopodia formation. A pseudogene of this gene is found on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]