TRBP (TARBP2) (NM_004178) Human Tagged ORF Clone

CAT#: RC201043

TARBP2 (Myc-DDK-tagged)-Human TAR (HIV-1) RNA binding protein 2 (TARBP2), transcript variant 3

Product Images

Circular map for RC201043

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TARBP2 (Cat# RC201043, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TARBP2 (Cat# TA808481)(1:2000). Positive lysates LY418167 (100ug) and LC418167 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY408744) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213967 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRBP
• Synonyms: LOQS; TRBP; TRBP1; TRBP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201043 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAAGAGGAGCAAGGCTCCGGCACTACCACGGGCTGCGGGCTGCCTAGTATAGAGCAAATGCTGG
  CCGCCAACCCAGGCAAGACCCCGATCAGCCTTCTGCAGGAGTATGGGACCAGAATAGGGAAGACGCCTGT
  GTACGACCTTCTCAAAGCCGAGGGCCAAGCCCACCAGCCTAATTTCACCTTCCGGGTCACCGTTGGCGAC
  ACCAGCTGCACTGGTCAGGGCCCCAGCAAGAAGGCAGCCAAGCACAAGGCAGCTGAGGTGGCCCTCAAAC
  ACCTCAAAGGGGGGAGCATGCTGGAGCCGGCCCTGGAGGACAGCAGTTCTTTTTCTCCCCTAGACTCTTC
  ACTGCCTGAGGACATTCCGGTTTTTACTGCTGCAGCAGCTGCTACCCCAGTTCCATCTGTAGTCCTAACC
  AGGAGCCCCCCCATGGAACTGCAGCCCCCTGTCTCCCCTCAGCAGTCTGAGTGCAACCCCGTTGGTGCTC
  TGCAGGAGCTGGTGGTGCAGAAAGGCTGGCGGTTGCCGGAGTACACAGTGACCCAGGAGTCTGGGCCAGC
  CCACCGCAAAGAATTCACCATGACCTGTCGAGTGGAGCGTTTCATTGAGATTGGGAGTGGCACTTCCAAA
  AAATTGGCAAAGCGGAATGCGGCGGCCAAAATGCTGCTTCGAGTGCACACGGTGCCTCTGGATGCCCGGG
  ATGGCAATGAGGTGGAGCCTGATGATGACCACTTCTCCATTGGTGTGGGCTCCCGCCTGGATGGTCTTCG
  AAACCGGGGCCCAGGTTGCACCTGGGATTCTCTACGAAATTCAGTAGGAGAGAAGATCCTGTCCCTCCGC
  AGTTGCTCCCTGGGCTCCCTGGGTGCCCTGGGCCCTGCCTGCTGCCGTGTCCTCAGTGAGCTCTCTGAGG
  AGCAGGCCTTTCACGTCAGCTACCTGGATATTGAGGAGCTGAGCCTGAGTGGACTCTGCCAGTGCCTGGT
  GGAACTGTCCACCCAGCCGGCCACTGTGTGTCATGGCTCTGCAACCACCAGGGAGGCAGCCCGTGGTGAG
  GCTGCCCGCCGTGCCCTGCAGTACCTCAAGATCATGGCAGGCAGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201043 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSEEEQGSGTTTGCGLPSIEQMLAANPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQPNFTFRVTVGD
  TSCTGQGPSKKAAKHKAAEVALKHLKGGSMLEPALEDSSSFSPLDSSLPEDIPVFTAAAAATPVPSVVLT
  RSPPMELQPPVSPQQSECNPVGALQELVVQKGWRLPEYTVTQESGPAHRKEFTMTCRVERFIEIGSGTSK
  KLAKRNAAAKMLLRVHTVPLDARDGNEVEPDDDHFSIGVGSRLDGLRNRGPGCTWDSLRNSVGEKILSLR
  SCSLGSLGALGPACCRVLSELSEEQAFHVSYLDIEELSLSGLCQCLVELSTQPATVCHGSATTREAARGE
  AARRALQYLKIMAGSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004178
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004178.2
• RefSeq Size: 1448 bp
• RefSeq ORF: 1038 bp
• Locus ID: 6895
• UniProt ID: Q15633
• Cytogenetics: 12q13.13
• Domains: DSRM
• Protein Families: Stem cell - Pluripotency
• MW: 39 kDa
• Gene Summary: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene. [provided by RefSeq, Jul 2008]