H2AW (NM_033445) Human Tagged ORF Clone

CAT#: RC200984

HIST3H2A (Myc-DDK-tagged)-Human histone cluster 3, H2a (HIST3H2A)

Product Images

Circular map for RC200984

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  Western blot validation of overexpression lysate (Cat# LY409510) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200984 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HIST3H2A protein (Cat# TP300984). The protein was produced from HEK293T cells transfected with HIST3H2A cDNA clone (Cat# RC200984) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: H2AW
• Synonyms: HIST3H2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200984 representing NM_033445
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGTCGTGGTAAGCAGGGTGGCAAGGCGCGCGCCAAGGCTAAGTCGCGCTCGTCGCGCGCGGGGC
  TGCAGTTCCCCGTGGGCCGCGTGCACCGGTTGCTCCGCAAGGGCAACTATTCGGAGCGCGTGGGCGCCGG
  CGCCCCGGTCTATCTGGCCGCGGTGCTCGAGTACTTGACTGCCGAGATCCTGGAGCTTGCCGGCAACGCG
  GCGCGCGACAACAAGAAGACGCGCATCATCCCGCGCCACCTGCAGCTGGCCATCCGCAACGACGAGGAGC
  TCAACAAGCTGCTGGGCCGCGTGACCATCGCGCAGGGTGGCGTCCTGCCCAACATCCAGGCCGTACTGCT
  GCCCAAGAAGACGGAGAGCCACCACAAGGCCAAGGGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200984 representing NM_033445
  Red=Cloning site Green=Tags(s)
  
  MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNA
  ARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033445
• ORF Size: 390 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033445.3
• RefSeq Size: 496 bp
• RefSeq ORF: 393 bp
• Locus ID: 92815
• UniProt ID: Q7L7L0
• Cytogenetics: 1q42.13
• Domains: H2A, histone
• Protein Pathways: Systemic lupus erythematosus
• MW: 13.9 kDa
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015]