MEPCE (NM_019606) Human Tagged ORF Clone

CAT#: RC200948

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MEPCE (Myc-DDK-tagged)-Human methylphosphate capping enzyme (MEPCE), transcript variant 1



  "NM_019606" in other vectors (7)

Reconstitution Protocol

USD 631.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Other products for "MEPCE"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MEPCE
Synonyms BCDIN3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200948 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCGAGATGGCGGCGGAGAAGGAGCCGTTTCTGGTGCCGGCCCCGCCGCCGCCGCTCAAAGATGAGT
CGGGCGGAGGGGGCGGCCCCACGGTGCCACCGCACCAAGAGGCCGCCTCTGGGGAGCTCCGCGGCGGGAC
GGAGCGTGGTCCGGGTCGTTGCGCGCCATCTGCGGGGTCCCCAGCCGCTGCGGTCGGTCGGGAAAGCCCC
GGGGCCGCGGCCACCTCCTCCAGTGGTCCCCAGGCGCAGCAGCACCGAGGGGGCGGCCCCCAGGCGCAGT
CGCATGGGGAGGCCCGCCTGTCGGATCCCCCGGGGCGAGCCGCTCCCCCGGACGTGGGGGAGGAGCGCCG
GGGAGGGGGCGGGACAGAGCTGGGTCCCCCTGCTCCTCCTCGACCCCGCAATGGCTATCAGCCCCACCGG
CCACCTGGGGGGGGCGGGGGCAAGAGGAGAAATAGCTGTAATGTAGGGGGAGGCGGGGGAGGCTTCAAAC
ATCCGGCCTTCAAGAGGCGCAGGCGGGTGAATTCGGACTGTGACTCTGTGTTACCCTCCAACTTCCTCCT
GGGGGGCAATATCTTTGATCCCCTGAACCTGAATAGCCTCCTGGATGAGGAAGTGAGCCGCACTCTCAAC
GCGGAGACCCCTAAGTCATCCCCCCTTCCGGCCAAAGGGCGAGATCCGGTGGAGATCCTCATCCCCAAAG
ATATTACTGACCCGCTCAGTCTCAATACTTGCACTGATGAGGGCCATGTAGTTCTTGCTTCGCCACTCAA
GACTGGTCGGAAGCGGCATAGACACCGGGGACAGCACCACCAGCAGCAGCAGGCAGCCGGAGGGAGTGAG
AGTCACCCCGTGCCGCCCACAGCCCCTCTCACCCCCTTACTCCACGGGGAGGGCGCCTCACAGCAGCCGC
GGCACAGGGGCCAGAACCGGGATGCCCCCCAACCCTATGAACTCAACACAGCCATCAACTGCAGGGATGA
AGTGGTGTCTCCCCTTCCATCTGCTCTGCAGGGTCCCTCAGGCTCCCTATCAGCCCCTCCAGCTGCCTCA
GTTATCTCTGCACCCCCATCTTCCTCCTCCCGACATCGCAAACGTCGCAGGACTTCCAGCAAGTCGGAGG
CAGGGGCTAGGGGTGGAGGCCAGGGTTCCAAGGAAAAGGGCCGAGGGAGTTGGGGAGGCCGCCACCACCA
CCACCACCCACTGCCTGCAGCAGGCTTCAAAAAGCAACAGCGCAAGTTCCAGTATGGGAATTATTGCAAA
TACTATGGGTACCGCAATCCTTCCTGTGAGGATGGGCGCCTTCGGGTGTTGAAGCCTGAGTGGTTTCGGG
GCCGGGACGTCCTAGATCTGGGCTGCAATGTGGGCCATCTGACCCTGAGCATTGCCTGCAAGTGGGGCCC
GTCCCGCATGGTGGGCCTGGATATCGATTCCCGGCTCATCCATTCTGCCCGCCAAAACATCCGACACTAC
CTTTCCGAGGAGCTGCGTCTCCCACCCCAGACTTTGGAAGGGGACCCGGGGGCAGAGGGTGAGGAAGGGA
CCACCACCGTTCGAAAGAGGAGCTGCTTCCCAGCCTCGCTGACTGCCAGCCGGGGTCCCATCGCTGCCCC
CCAAGTGCCCTTGGATGGAGCGGACACATCAGTCTTCCCCAACAATGTTGTCTTCGTCACGGGTAATTAT
GTGCTGGATCGAGATGACCTGGTGGAGGCCCAAACACCTGAGTATGATGTGGTGCTCTGCCTCAGCCTCA
CCAAGTGGGTGCATCTGAACTGGGGAGACGAGGGCCTGAAGCGCATGTTTCGCCGGATCTACCGGCACCT
ACGCCCTGGGGGCATCCTGGTCCTAGAGCCCCAACCCTGGTCGTCGTATGGCAAGAGAAAGACTCTTACA
GAAACGATCTACAAGAACTACTACCGAATCCAATTGAAGCCAGAGCAGTTCAGTTCCTACCTGACATCCC
CAGACGTGGGCTTCTCCAGCTATGAGCTTGTGGCCACACCCCACAACACCTCTAAAGGCTTCCAGCGTCC
TGTGTACCTGTTCCACAAGGCCCGATCCCCCAGCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200948 protein sequence
Red=Cloning site Green=Tags(s)

MIEMAAEKEPFLVPAPPPPLKDESGGGGGPTVPPHQEAASGELRGGTERGPGRCAPSAGSPAAAVGRESP
GAAATSSSGPQAQQHRGGGPQAQSHGEARLSDPPGRAAPPDVGEERRGGGGTELGPPAPPRPRNGYQPHR
PPGGGGGKRRNSCNVGGGGGGFKHPAFKRRRRVNSDCDSVLPSNFLLGGNIFDPLNLNSLLDEEVSRTLN
AETPKSSPLPAKGRDPVEILIPKDITDPLSLNTCTDEGHVVLASPLKTGRKRHRHRGQHHQQQQAAGGSE
SHPVPPTAPLTPLLHGEGASQQPRHRGQNRDAPQPYELNTAINCRDEVVSPLPSALQGPSGSLSAPPAAS
VISAPPSSSSRHRKRRRTSSKSEAGARGGGQGSKEKGRGSWGGRHHHHHPLPAAGFKKQQRKFQYGNYCK
YYGYRNPSCEDGRLRVLKPEWFRGRDVLDLGCNVGHLTLSIACKWGPSRMVGLDIDSRLIHSARQNIRHY
LSEELRLPPQTLEGDPGAEGEEGTTTVRKRSCFPASLTASRGPIAAPQVPLDGADTSVFPNNVVFVTGNY
VLDRDDLVEAQTPEYDVVLCLSLTKWVHLNWGDEGLKRMFRRIYRHLRPGGILVLEPQPWSSYGKRKTLT
ETIYKNYYRIQLKPEQFSSYLTSPDVGFSSYELVATPHNTSKGFQRPVYLFHKARSPSH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019606
ORF Size 2067 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019606.6
RefSeq Size 3047 bp
RefSeq ORF 2070 bp
Locus ID 56257
UniProt ID Q7L2J0
Cytogenetics 7q22.1
Protein Families Druggable Genome
MW 74.4 kDa
Gene Summary S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it.[UniProtKB/Swiss-Prot Function]

Other Versions

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