GMCL1 (NM_178439) Human Tagged ORF Clone

CAT#: RC200928

GMCL1 (Myc-DDK-tagged)-Human germ cell-less homolog 1 (Drosophila) (GMCL1)

Product Images

Circular map for RC200928

• 

  Western blot validation of overexpression lysate (Cat# LY405961) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200928 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GMCL1
• Synonyms: BTBD13; GCL; GCL1; SPATA29
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200928 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATCGTTGAGCAGCCGGGTGCTGCGCCAGCCAAGACCAGCCCTTGCCCAGCAGGCGCAGGGTGCCA
  GGGCGGGGGGCTCGGCCCGGAGGCCGGACACTGGAGACGATGCGGCGGGCCACGGATTCTGTTACTGTGC
  GGGCAGCCACAAGCGCAAGCGGAGCAGCGGGTCCTTCTGCTACTGTCACCCTGACTCGGAGACGGACGAG
  GATGAGGAGGAGGGGGACGAGCAGCAGCGGCTCCTCAACACCCCTCGAAGGAAAAAATTAAAGAGTACAT
  CTAAATATATTTATCAAACATTATTTTTGAATGGTGAAAACAGTGACATTAAGATTTGTGCTCTAGGAGA
  AGAATGGAGCTTACACAAAATATATTTATGTCAATCTGGCTACTTTTCTAGTATGTTCAGTGGTTCTTGG
  AAAGAATCCAGCATGAATATTATTGAACTGGAGATTCCTGACCAGAACATTGATGTAGAAGCACTGCAGG
  TTGCATTTGGTTCACTGTATCGAGATGATGTCTTGATAAAGCCCAGTCGAGTTGTTGCCATTTTGGCAGC
  AGCTTGTTTGCTGCAGTTGGACGGTTTAATACAGCAGTGTGGTGAGACAATGAAGGAAACAGTTAATGTG
  AAAACTGTATGTGGCTATTACACATCAGCAGGGACCTATGGATTAGATTCTGTAAAGAAAAAGTGCCTTG
  AATGGCTTCTAAACAATTTGATGACTCACCAGAATGTTGAACTTTTTAAAGAACTCAGTATAAATGTCAT
  GAAACAGCTCATTGGTTCATCTAACTTATTTGTGATGCAAGTGGAGATGGATATATACACTGCTCTAAAA
  AAGTGGATGTTCCTTCAACTTGTGCCTTCTTGGAATGGATCTTTAAAACAGCTTTTGACAGAAACAGATG
  TCTGGTTTTCTAAACAGAGGAAAGATTTTGAAGGTATGGCCTTTCTTGAAACTGAACAAGGAAAACCATT
  TGTGTCAGTATTCAGACATTTAAGGTTACAATATATTATCAGTGATCTGGCTTCTGCAAGAATTATTGAA
  CAAGATGCTGTAGTACCTTCAGAATGGCTCTCTTCTGTGTATAAACAGCAGTGGTTTGCTATGCTGCGGG
  CAGAACAGGACAGTGAGGTGGGGCCTCAAGAAATCAATAAAGAAGAACTAGAGGGAAACAGCATGAGGTG
  TGGTAGAAAGCTTGCCAAAGATGGTGAATACTGCTGGCGTTGGACAGGTTTTAACTTCGGCTTCGACCTA
  CTTGTAACTTACACCAATCGATACATCATTTTCAAACGCAATACACTGAATCAGCCATGTAGCGGATCTG
  TCAGTTTACAGCCTCGAAGGAGCATAGCATTTAGATTACGTTTGGCTTCTTTTGATAGTAGTGGAAAACT
  AATATGTAGTAGAACAACTGGCTATCAAATACTTACACTTGAAAAGGATCAGGAACAAGTGGTGATGAAC
  TTGGACAGCAGGCTTCTGATCTTCCCTTTATATATCTGCTGTAACTTCTTGTATATATCACCAGAAAAAA
  AGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200928 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSLSSRVLRQPRPALAQQAQGARAGGSARRPDTGDDAAGHGFCYCAGSHKRKRSSGSFCYCHPDSETDE
  DEEEGDEQQRLLNTPRRKKLKSTSKYIYQTLFLNGENSDIKICALGEEWSLHKIYLCQSGYFSSMFSGSW
  KESSMNIIELEIPDQNIDVEALQVAFGSLYRDDVLIKPSRVVAILAAACLLQLDGLIQQCGETMKETVNV
  KTVCGYYTSAGTYGLDSVKKKCLEWLLNNLMTHQNVELFKELSINVMKQLIGSSNLFVMQVEMDIYTALK
  KWMFLQLVPSWNGSLKQLLTETDVWFSKQRKDFEGMAFLETEQGKPFVSVFRHLRLQYIISDLASARIIE
  QDAVVPSEWLSSVYKQQWFAMLRAEQDSEVGPQEINKEELEGNSMRCGRKLAKDGEYCWRWTGFNFGFDL
  LVTYTNRYIIFKRNTLNQPCSGSVSLQPRRSIAFRLRLASFDSSGKLICSRTTGYQILTLEKDQEQVVMN
  LDSRLLIFPLYICCNFLYISPEKKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178439
• ORF Size: 1545 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178439.5
• RefSeq Size: 2402 bp
• RefSeq ORF: 1548 bp
• Locus ID: 64395
• UniProt ID: Q96IK5
• Cytogenetics: 2p13.3
• MW: 58.7 kDa
• Gene Summary: This gene encodes a nuclear envelope protein that appears to be involved in spermatogenesis, either directly or by influencing genes that play a more direct role in the process. This multi-exon locus is the homolog of the mouse and drosophila germ cell-less gene but the human genome also contains a single-exon locus on chromosome 5 that contains an open reading frame capable of encoding a highly-related protein. [provided by RefSeq, Jul 2008]