RRM1 (NM_001033) Human Tagged ORF Clone

CAT#: RC200726

RRM1 (Myc-DDK-tagged)-Human ribonucleotide reductase M1 (RRM1)

Product Images

Circular map for RC200726

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RRM1 (Cat# RC200726, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RRM1 (Cat# TA804382)(1:500). Positive lysates LY422336 (100ug) and LC422336 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY422336) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200726 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RRM1 protein (Cat# TP300726). The protein was produced from HEK293T cells transfected with RRM1 cDNA clone (Cat# RC200726) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RRM1
• Synonyms: R1; RIR1; RR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200726 representing NM_001033.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCATGTGATCAAGCGAGATGGCCGCCAAGAACGAGTCATGTTTGACAAAATTACATCTCGAATCCAG
  AAGCTTTGTTATGGACTCAATATGGATTTTGTTGATCCTGCTCAGATCACCATGAAAGTAATCCAAGGC
  TTGTACAGTGGGGTCACCACAGTGGAACTAGATACTTTGGCTGCTGAAACAGCTGCAACCTTGACTACT
  AAGCACCCTGACTATGCTATCCTGGCAGCCAGGATCGCTGTCTCTAACTTGCACAAAGAAACAAAGAAA
  GTGTTCAGTGATGTGATGGAAGACCTCTATAACTACATAAATCCACATAATGGCAAACACTCTCCCATG
  GTGGCCAAGTCAACATTGGATATTGTTCTGGCCAATAAAGATCGCCTGAATTCTGCTATTATCTATGAC
  CGAGATTTCTCTTACAATTACTTCGGCTTTAAGACGCTAGAGCGGTCTTATTTGTTGAAGATCAATGGA
  AAAGTGGCTGAAAGACCACAACATATGTTGATGAGAGTATCTGTTGGGATCCACAAAGAAGACATTGAT
  GCAGCAATTGAAACATATAATCTTCTTTCTGAGAGGTGGTTTACTCATGCTTCGCCCACTCTCTTCAAT
  GCTGGTACCAACCGCCCACAACTTTCTAGCTGTTTTCTTCTGAGTATGAAAGATGACAGCATTGAAGGC
  ATTTATGACACTCTAAAGCAATGTGCATTGATTTCTAAGTCTGCTGGAGGAATTGGTGTTGCTGTGAGT
  TGTATTCGGGCTACTGGCAGCTACATTGCTGGGACTAATGGCAATTCCAATGGCCTTGTACCGATGCTG
  AGAGTATATAACAACACAGCTAGATATGTGGATCAAGGTGGGAACAAGCGTCCTGGGGCATTTGCTATT
  TACCTGGAGCCTTGGCATTTAGACATCTTTGAATTCCTTGATTTAAAGAAGAACACAGGAAAGGAAGAG
  CAGCGTGCCAGAGATCTTTTCTTTGCTCTTTGGATTCCGGATCTCTTCATGAAACGAGTGGAGACTAAT
  CAGGACTGGTCTTTGATGTGTCCAAATGAGTGTCCTGGTCTGGATGAGGTTTGGGGAGAGGAATTTGAG
  AAACTATATGCAAGTTATGAGAAACAAGGTCGTGTCCGCAAAGTTGTAAAAGCTCAGCAGCTTTGGTAT
  GCCATCATTGAGTCTCAGACGGAAACAGGCACCCCGTATATGCTCTACAAAGATTCCTGTAATCGAAAG
  AGCAACCAGCAGAACCTGGGAACCATCAAATGCAGCAACCTGTGCACAGAAATAGTGGAGTACACCAGC
  AAAGATGAGGTTGCTGTTTGTAATTTGGCTTCCCTGGCCCTGAATATGTATGTCACATCAGAACACACA
  TACGACTTTAAGAAGTTGGCTGAAGTCACTAAAGTCGTTGTCCGAAACTTGAATAAAATTATTGATATA
  AACTACTATCCTGTACCAGAGGCATGCCTATCAAATAAACGCCATCGCCCCATTGGAATTGGGGTACAA
  GGTCTGGCAGATGCTTTTATCCTGATGAGATACCCTTTTGAGAGTGCAGAAGCCCAGTTACTGAATAAG
  CAGATCTTTGAAACTATTTATTATGGTGCTCTGGAAGCCAGCTGTGACCTTGCCAAGGAGCAGGGCCCA
  TACGAAACCTATGAGGGCTCTCCAGTTAGCAAAGGAATTCTTCAGTATGATATGTGGAATGTTACTCCT
  ACAGACCTATGGGACTGGAAGGTTCTCAAGGAGAAGATTGCAAAGTATGGTATAAGAAACAGTTTACTT
  ATTGCCCCGATGCCTACAGCTTCCACTGCTCAGATCCTGGGGAATAATGAGTCCATTGAACCTTACACC
  AGCAACATCTATACTCGCAGAGTCTTGTCAGGAGAATTTCAGATTGTAAATCCTCACTTATTGAAAGAT
  CTTACCGAGCGGGGCCTATGGCATGAAGAGATGAAAAACCAGATTATTGCATGCAATGGCTCTATTCAG
  AGCATACCAGAAATTCCTGATGACCTGAAGCAACTTTATAAAACTGTGTGGGAAATCTCTCAGAAAACT
  GTTCTCAAGATGGCAGCTGAGAGAGGTGCTTTCATTGATCAAAGCCAATCTTTGAACATCCACATTGCT
  GAGCCTAACTATGGCAAACTCACTAGTATGCACTTCTACGGCTGGAAGCAGGGTTTGAAGACTGGGATG
  TATTATTTAAGGACGAGACCAGCAGCTAATCCAATCCAGTTCACTCTAAATAAGGAGAAGCTAAAAGAT
  AAAGAAAAGGTATCAAAAGAGGAAGAAGAGAAGGAGAGGAACACAGCAGCCATGGTGTGCTCTTTGGAG
  AATAGAGATGAATGTCTGATGTGTGGATCC
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC200726
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MHVIKRDGRQERVMFDKITSRIQKLCYGLNMDFVDPAQITMKVIQGLYSGVTTVELDTLAAETAATLTT
  KHPDYAILAARIAVSNLHKETKKVFSDVMEDLYNYINPHNGKHSPMVAKSTLDIVLANKDRLNSAIIYD
  RDFSYNYFGFKTLERSYLLKINGKVAERPQHMLMRVSVGIHKEDIDAAIETYNLLSERWFTHASPTLFN
  AGTNRPQLSSCFLLSMKDDSIEGIYDTLKQCALISKSAGGIGVAVSCIRATGSYIAGTNGNSNGLVPML
  RVYNNTARYVDQGGNKRPGAFAIYLEPWHLDIFEFLDLKKNTGKEEQRARDLFFALWIPDLFMKRVETN
  QDWSLMCPNECPGLDEVWGEEFEKLYASYEKQGRVRKVVKAQQLWYAIIESQTETGTPYMLYKDSCNRK
  SNQQNLGTIKCSNLCTEIVEYTSKDEVAVCNLASLALNMYVTSEHTYDFKKLAEVTKVVVRNLNKIIDI
  NYYPVPEACLSNKRHRPIGIGVQGLADAFILMRYPFESAEAQLLNKQIFETIYYGALEASCDLAKEQGP
  YETYEGSPVSKGILQYDMWNVTPTDLWDWKVLKEKIAKYGIRNSLLIAPMPTASTAQILGNNESIEPYT
  SNIYTRRVLSGEFQIVNPHLLKDLTERGLWHEEMKNQIIACNGSIQSIPEIPDDLKQLYKTVWEISQKT
  VLKMAAERGAFIDQSQSLNIHIAEPNYGKLTSMHFYGWKQGLKTGMYYLRTRPAANPIQFTLNKEKLKD
  KEKVSKEEEEKERNTAAMVCSLENRDECLMCGS
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC200726 also available, TP300726
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033
• ORF Size: 2376 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3117 bp
• RefSeq ORF: 2379 bp
• Locus ID: 6240
• UniProt ID: P23921
• Cytogenetics: 11p15.4
• Domains: ribonuc_red_lg, ATP-cone
• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways, Purine metabolism, Pyrimidine metabolism
• MW: 90.1 kDa
• Gene Summary: This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]