MEST (NM_002402) Human Tagged ORF Clone

CAT#: RC200615

MEST (Myc-DDK-tagged)-Human mesoderm specific transcript homolog (mouse) (MEST), transcript variant 1

Product Images

Circular map for RC200615

• 

  Western blot validation of overexpression lysate (Cat# LY419353) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200615 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MEST
• Synonyms: PEG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200615 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCGCCGAGATCGCCTCCGCAGGATGAGGGAGTGGTGGGTCCAGGTGGGGCTGCTGGCCGTGCCCC
  TGCTTGCTGCGTACCTGCACATCCCACCCCCTCAGCTCTCCCCTGCCCTTCACTCATGGAAGTCTTCAGG
  CAAGTTTTTCACTTACAAGGGACTGCGTATCTTCTACCAAGACTCTGTGGGTGTGGTTGGAAGTCCAGAG
  ATAGTTGTGCTTTTACACGGTTTTCCAACATCCAGCTACGACTGGTACAAGATTTGGGAAGGTCTGACCT
  TGAGGTTTCATCGGGTGATTGCCCTTGATTTCTTAGGCTTTGGCTTCAGTGACAAACCGAGACCACATCA
  CTATTCCATATTTGAGCAGGCCAGCATCGTGGAAGCGCTTTTGCGGCATCTGGGGCTCCAGAACCGCAGG
  ATCAACCTTCTTTCTCATGACTATGGAGATATTGTTGCTCAGGAGCTTCTCTACAGGTACAAGCAGAATC
  GATCTGGTCGGCTTACCATAAAGAGTCTCTGTCTGTCAAATGGAGGTATCTTTCCTGAGACTCACCGTCC
  ACTCCTTCTCCAAAAGCTACTCAAAGATGGAGGTGTGCTGTCACCCATCCTCACACGACTGATGAACTTC
  TTTGTATTCTCTCGAGGTCTCACCCCAGTCTTTGGGCCGTATACTCGGCCCTCTGAGAGTGAGCTGTGGG
  ACATGTGGGCAGGGATCCGCAACAATGACGGGAACTTAGTCATTGACAGTCTCTTACAGTACATCAATCA
  GAGGAAGAAGTTCAGAAGGCGCTGGGTGGGAGCTCTTGCCTCTGTAACTATCCCCATTCATTTTATCTAT
  GGGCCATTGGATCCTGTAAATCCCTATCCAGAGTTTTTGGAGCTGTACAGGAAAACGCTGCCGCGGTCCA
  CAGTGTCGATTCTGGATGACCACATTAGCCACTATCCACAGCTAGAGGATCCCATGGGCTTCTTGAATGC
  ATATATGGGCTTCATCAACTCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200615 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVRRDRLRRMREWWVQVGLLAVPLLAAYLHIPPPQLSPALHSWKSSGKFFTYKGLRIFYQDSVGVVGSPE
  IVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRPHHYSIFEQASIVEALLRHLGLQNRR
  INLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPLLLQKLLKDGGVLSPILTRLMNF
  FVFSRGLTPVFGPYTRPSESELWDMWAGIRNNDGNLVIDSLLQYINQRKKFRRRWVGALASVTIPIHFIY
  GPLDPVNPYPEFLELYRKTLPRSTVSILDDHISHYPQLEDPMGFLNAYMGFINSF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002402
• ORF Size: 1005 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002402.4
• RefSeq Size: 2513 bp
• RefSeq ORF: 1008 bp
• Locus ID: 4232
• UniProt ID: Q5EB52
• Cytogenetics: 7q32.2
• Domains: abhydrolase
• Protein Families: Protease, Transmembrane
• MW: 38.8 kDa
• Gene Summary: This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011]