CRMP1 (NM_001313) Human Tagged ORF Clone

CAT#: RC200483

CRMP1 (Myc-DDK-tagged)-Human collapsin response mediator protein 1 (CRMP1), transcript variant 2

Product Images

Circular map for RC200483

• 

  Western blot validation of overexpression lysate (Cat# LY400522) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200483 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CRMP1 protein (Cat# TP300483). The protein was produced from HEK293T cells transfected with CRMP1 cDNA clone (Cat# RC200483) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRMP1
• Synonyms: CRMP-1; DPYSL1; DRP-1; DRP1; ULIP-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200483 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTACCAGGGCAAGAAGAGCATCCCGCACATCACGAGTGACCGACTCCTCATCAAAGGTGGACGGA
  TCATCAACGATGACCAATCCCTTTATGCTGACGTCTACCTGGAGGATGGACTTATCAAACAAATAGGAGA
  GAACTTAATCGTTCCTGGTGGAGTGAAGACCATTGAAGCCAACGGGCGGATGGTTATTCCCGGAGGTATT
  GATGTCAACACGTACCTGCAGAAGCCCTCCCAGGGGATGACTGCGGCTGATGACTTCTTCCAAGGGACCA
  GGGCGGCACTGGTGGGCGGGACCACGATGATCATTGACCATGTTGTTCCTGAACCTGGGTCCAGCCTACT
  GACCTCTTTCGAGAAGTGGCACGAAGCAGCTGACACCAAATCCTGCTGTGATTACTCCCTCCACGTGGAC
  ATCACAAGCTGGTACGATGGCGTTCGGGAGGAGCTGGAGGTGCTGGTGCAGGACAAAGGCGTCAATTCCT
  TCCAAGTCTACATGGCCTATAAGGATGTCTACCAAATGTCCGACAGCCAGCTCTATGAAGCCTTTACCTT
  CCTTAAGGGCCTGGGAGCTGTGATCTTGGTCCATGCAGAAAATGGAGATTTGATAGCTCAGGAACAAAAG
  CGGATCCTGGAGATGGGCATCACGGGTCCCGAGGGCCATGCCCTGAGCAGACCTGAAGAGCTGGAGGCCG
  AGGCGGTGTTCCGGGCCATCACCATTGCGGGCCGGATCAACTGCCCTGTGTACATCACCAAGGTCATGAG
  CAAGAGTGCAGCCGACATCATCGCTCTGGCCAGGAAGAAAGGGCCCCTAGTTTTTGGAGAGCCCATTGCC
  GCCAGCCTGGGGACCGATGGCACCCATTACTGGAGCAAGAACTGGGCCAAGGCTGCGGCGTTCGTGACTT
  CCCCTCCCCTGAGCCCGGACCCTACCACGCCCGACTACTTGACCTCCCTACTGGCCTGTGGGGACTTGCA
  GGTCACAGGCAGCGGCCACTGTCCCTACAGCACTGCCCAGAAGGCGGTGGGCAAGGACAACTTTACCCTG
  ATCCCCGAGGGTGTCAACGGGATAGAGGAGCGGATGACGGTCGTCTGGGACAAGGCGGTGGCTACTGGCA
  AAATGGATGAGAACCAGTTTGTCGCTGTCACCAGCACCAATGCAGCCAAGATCTTTAACCTGTACCCAAG
  GAAAGGGCGGATTGCCGTGGGCTCGGATGCCGACGTGGTCATCTGGGACCCCGACAAGTTGAAGACCATA
  ACAGCCAAAAGTCACAAGTCGGCGGTGGAGTACAACATCTTCGAGGGTATGGAGTGCCACGGCTCCCCAC
  TAGTGGTCATCAGCCAGGGCAAGATCGTCTTTGAAGACGGAAACATCAACGTCAACAAGGGCATGGGCCG
  CTTCATTCCGCGGAAGGCGTTCCCGGAGCACCTGTACCAGCGCGTCAAAATCAGGAATAAGGTTTTTGGA
  TTGCAAGGGGTTTCCAGGGGCATGTATGACGGTCCTGTGTACGAGGTACCAGCTACACCCAAATATGCAA
  CTCCCGCTCCTTCAGCCAAATCTTCGCCTTCTAAACACCAGCCCCCACCCATCAGAAACCTCCACCAGTC
  CAACTTCAGCTTATCAGGTGCCCAGATAGATGACAACAATCCCAGGCGCACCGGCCACCGCATCGTGGCG
  CCCCCTGGTGGCCGCTCCAACATCACCAGCCTCGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200483 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSYQGKKSIPHITSDRLLIKGGRIINDDQSLYADVYLEDGLIKQIGENLIVPGGVKTIEANGRMVIPGGI
  DVNTYLQKPSQGMTAADDFFQGTRAALVGGTTMIIDHVVPEPGSSLLTSFEKWHEAADTKSCCDYSLHVD
  ITSWYDGVREELEVLVQDKGVNSFQVYMAYKDVYQMSDSQLYEAFTFLKGLGAVILVHAENGDLIAQEQK
  RILEMGITGPEGHALSRPEELEAEAVFRAITIAGRINCPVYITKVMSKSAADIIALARKKGPLVFGEPIA
  ASLGTDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYLTSLLACGDLQVTGSGHCPYSTAQKAVGKDNFTL
  IPEGVNGIEERMTVVWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDADVVIWDPDKLKTI
  TAKSHKSAVEYNIFEGMECHGSPLVVISQGKIVFEDGNINVNKGMGRFIPRKAFPEHLYQRVKIRNKVFG
  LQGVSRGMYDGPVYEVPATPKYATPAPSAKSSPSKHQPPPIRNLHQSNFSLSGAQIDDNNPRRTGHRIVA
  PPGGRSNITSLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001313
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001313.5
• RefSeq Size: 3074 bp
• RefSeq ORF: 1719 bp
• Locus ID: 1400
• UniProt ID: Q14194
• Cytogenetics: 4p16.2
• Domains: Amidohydro_1
• MW: 62.2 kDa
• Gene Summary: This gene encodes a member of a family of cytosolic phosphoproteins expressed exclusively in the nervous system. The encoded protein is thought to be a part of the semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]